Establishment of in vivo metastasis model of human adenoid cystic carcinoma: detection of metastasis by PCR with human beta-globin gene

• Published in: Kobe journal of the medical sciences Vol. 48; no. 5-6; pp. 145 - 152
• Main Authors: Komatsubara, Hideki, Umeda, Masahiro, Oku, Naohisa, Komori, Takahide
• Format: Journal Article
• Language: English
• Published: Japan 01.12.2002
• Subjects: Animals; Base Sequence; Biopsy, Needle; Carcinoma, Adenoid Cystic - pathology; Carcinoma, Adenoid Cystic - secondary; Disease Models, Animal; Globins - genetics; Humans; Immunohistochemistry; Lung Neoplasms - pathology; Lung Neoplasms - secondary; Male; Mice; Mice, Inbred BALB C; Molecular Sequence Data; Neoplasm Invasiveness - pathology; Neoplasm Transplantation; Polymerase Chain Reaction - methods; Salivary Gland Neoplasms - pathology; Sensitivity and Specificity; Transplantation, Heterologous
• ISSN: 0023-2513

Cells from the human adenoid cystic carcinoma (ACC) line, KOA-1, originating from the minor salivary gland and showing histological features of a dominantly solid pattern of ACC, were transplanted into the subcutaneous tissue of nude mice. The occurrence of lung metastasis was then examined both histologically and by PCR with a human-specific base arrangement of the beta-globin gene. Histologic examinations revealed lung metastasis in 5 of 30 mice. On the other hand, PCR showed a higher positive rate: 26 of 67 mice (0/10 at 1 month, 5/15 at 2 months, and 21/42 at 3 months after the transplantation). When some lungs which showed positive results by PCR analysis were transplanted into other mice, KOA-1 tumors developed. The results suggested that this experimental model could be a standard in vivo model of metastasis of human ACC.