発達期の低酸素状態は脳内の発達障害関連部位においてアストロサイトを低下させる

The fetal hypoxia such as threatened abortion is one of the risk factors for neurodevelopmental disorder. To clarify the pathophysiological mechanism in onset of these disease caused by hypoxic stress, we generated a rat model of fetal hypoxic stress and analyzed the expression level of astrocytes i...

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Published in日本薬理学会年会要旨集 p. 1-O-C2-1
Main Authors 冨田, 修平, 徳留, 健太郎, 山口, 雄大, 松永, 慎司, 植木, 正明
Format Journal Article
LanguageJapanese
Published 公益社団法人 日本薬理学会 2021
Subjects
astrocyte
brain
hypoxia
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ISSN2435-4953
DOI10.1254/jpssuppl.94.0_1-O-C2-1

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Abstract The fetal hypoxia such as threatened abortion is one of the risk factors for neurodevelopmental disorder. To clarify the pathophysiological mechanism in onset of these disease caused by hypoxic stress, we generated a rat model of fetal hypoxic stress and analyzed the expression level of astrocytes in developmental disorder-related region. Pregnant F344 rats were treated with subchronic hypoxia stress. After birth, rats were reared in normal home cage and behavioral tests were performed. After behavioral tests, rat brains were removed and used for immunohistochemical staining with glial fibrillary acidic protein (GFAP), which is a marker protein of astrocytes. In behavioral tests, the time of social behavior was significantly shortened in fetal hypoxia stress-loaded rats (hypoxia rats) compared to that of control rats. In addition, hypoxia rats showed disruption of learning and memory function. Furthermore, immunohistochemical staining revealed that the number of GFAP positive cells was significantly decrease in the neurodevelopmental disease-related regions in hypoxia rats. These results indicated that decrease of astrocytes in such brain area might cause disruption of neural informational transmission, which showed neurodevelopmental disorder-like behavioral phenotypes.
AbstractList The fetal hypoxia such as threatened abortion is one of the risk factors for neurodevelopmental disorder. To clarify the pathophysiological mechanism in onset of these disease caused by hypoxic stress, we generated a rat model of fetal hypoxic stress and analyzed the expression level of astrocytes in developmental disorder-related region. Pregnant F344 rats were treated with subchronic hypoxia stress. After birth, rats were reared in normal home cage and behavioral tests were performed. After behavioral tests, rat brains were removed and used for immunohistochemical staining with glial fibrillary acidic protein (GFAP), which is a marker protein of astrocytes. In behavioral tests, the time of social behavior was significantly shortened in fetal hypoxia stress-loaded rats (hypoxia rats) compared to that of control rats. In addition, hypoxia rats showed disruption of learning and memory function. Furthermore, immunohistochemical staining revealed that the number of GFAP positive cells was significantly decrease in the neurodevelopmental disease-related regions in hypoxia rats. These results indicated that decrease of astrocytes in such brain area might cause disruption of neural informational transmission, which showed neurodevelopmental disorder-like behavioral phenotypes.
Author 植木, 正明
山口, 雄大
冨田, 修平
松永, 慎司
徳留, 健太郎
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  fullname: 松永, 慎司
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  fullname: 植木, 正明
  organization: 西脇市立病院・麻酔
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SubjectTerms astrocyte
brain
hypoxia
Title 発達期の低酸素状態は脳内の発達障害関連部位においてアストロサイトを低下させる
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