妊娠期低酸素曝露ラットの大脳皮質内における微小構造の解析

• Published in: 日本薬理学会年会要旨集 p. 2-B-P-028
• Main Authors: 徳留, 健太郎, 本間, 拓二郎, 松永, 慎司, 植木, 正明, 大野, 行弘, 冨田, 修平
• Format: Journal Article
• Language: Japanese
• Published: 公益社団法人 日本薬理学会 2023
• Subjects: glutamic acid; hypoxia; pregnancy; synapse
• ISSN: 2435-4953
• DOI: 10.1254/jpssuppl.97.0_2-B-P-028

Maternal hypoxic stress such as threatened abortion at early pregnancy is thought to be a risk factor for children’s neurodevelopmental disorder onset. We previously reported that maternal hypoxia caused neurodevelopmental disorder-like behavior in rat and both NeuN-positive cells and glial fibrillary acidic protein-positive cells were decreased in rat anterior cingulate cortex (ACC) by treating with maternal hypoxic stress, which indicated that ACC might be involved in the onset of hypoxia-related neurodevelopmental disorder. Here, we conducted the microstructural analysis in ACC by using immunohistochemical studies. First, to check which neural subtypes decrease in ACC, immunofluorescent double staining was conducted. As results, glutamatergic pyramidal neurons were decreased by maternal hypoxia. In addition, glutamatergic neural presynaptic marker VGLUT1 density was decrease around pyramidal neuron in hypoxia rats’ ACC. Furthermore, microstructural analysis by using transmission electron microscopy revealed that the number of presynaptic vesicles was decrease in hypoxia rat ACC. These results suggested that maternal hypoxia might cause abnormal behaviors by hypoactivity of glutamatergic neural networks.