SHRSPにおけるカノーラ油毒性の標的とその毒性に関与する機序

• Published in: 日本薬理学会年会要旨集 p. 1-P-114
• Main Authors: 立松, 憲次郎, 宮澤, 大介, 大原, 直樹, 内藤, 由紀子, 西川, 真衣, 奥山, 治美
• Format: Journal Article
• Language: Japanese
• Published: 公益社団法人 日本薬理学会 2020
• Subjects: renin-angiotensin system; steroid hormone; toxicity
• ISSN: 2435-4953
• DOI: 10.1254/jpssuppl.93.0_1-P-114

[Aim of the study] Canola oil (CAN) ingestion is known to shorten the life-span of male SHRSP and the life-shortening is preceded by a decrease in plasma testosterone and an increase in aldosterone. The present study was conducted to clarify the target of the toxicity. [Methods] Male SHRSP were given an AIN-93G diet containing 10w/w% soybean oil (Control) or CAN and tap water ad libitum for 8 weeks. At the 8th week the animals were sacrificed and plasma concentrations of testosterone, aldosterone, LH and FSH were measured using ELISA kits. Testes were isolated and histopathologically examined. Gene expressions for ACE and ACE2 in the lung, kidney and testis were measured. [Results and discussion] CAN-induced decrease in plasma testosterone and increase in aldosterone were confirmed. Both, plasma LH and FSH were similar between the Control and CAN groups. Leydig cell counts in the testis were comparable between the two groups. The ratios of gene expression for ACEs to that for ACE (ACE2/ACE) in both, the testis and the kidney of the CAN group were significantly lower than those in the Control group, while ACE2/ACE in the lung of the two groups were similar.These results demonstrate that CAN ingestion does not affect gonadotropin secretion from the pituitary while suppresses the testicular function without pathomorphological changes nor Leydig cell count. The decreased ACE2/ACE in the testis and the kidney of the CAN group may affect the local renin-angiotensin-aldosterone system (RAAS). In this presentation the relationship among testosterone, aldosterone and RAAS in the CAN toxicity is discussed.