ヒト口腔扁平上皮がん細胞において低分子干渉RNAを用いたヒートショック転写因子1の抑制はマイルドハイパーサーミアとハイパーサーミアの感受性を増感する

様々ながんに対して, 抗がん効果が期待できるハイパーサーミア (HT) が適用されている. しかしながら, ヒートショックタンパク質 (HSP) の誘導によりがん細胞が温熱耐性能を獲得し, HT効果を減弱させることが知られている. 近年, ヒートショック転写因子1 (HSF1) の抑制が温熱感受性を上昇させることがいくつかの種類のがん細胞で示された. 本研究では, ヒト口腔扁平上皮がん (OSCC) 細胞においてマイルドハイパーサーミア (MHT) とHTの感受性に対する低分子干渉RNA (siRNA) を用いたHSF1の抑制の効果を検討した. ヒトOSCCであるHSC-3細胞へのsiHSF1...

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Published inThermal Medicine Vol. 27; no. 4; pp. 99 - 108
Main Authors 大塚, 健三, 和田, 重人, 古澤, 之裕, 田渕, 圭章, 近藤, 隆
Format Journal Article
LanguageJapanese
Published 日本ハイパーサーミア学会 2011
Online AccessGet full text
ISSN1882-2576
1882-3750
DOI10.3191/thermalmed.27.99

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Abstract 様々ながんに対して, 抗がん効果が期待できるハイパーサーミア (HT) が適用されている. しかしながら, ヒートショックタンパク質 (HSP) の誘導によりがん細胞が温熱耐性能を獲得し, HT効果を減弱させることが知られている. 近年, ヒートショック転写因子1 (HSF1) の抑制が温熱感受性を上昇させることがいくつかの種類のがん細胞で示された. 本研究では, ヒト口腔扁平上皮がん (OSCC) 細胞においてマイルドハイパーサーミア (MHT) とHTの感受性に対する低分子干渉RNA (siRNA) を用いたHSF1の抑制の効果を検討した. ヒトOSCCであるHSC-3細胞へのsiHSF1-2 (HSF1に対するsiRNA) の添加は, 37°Cにおいて時間依存的にHSF1タンパク質レベルを抑制し, その添加48時間後でほぼ完全なHSF1のノックダウン効果を観察できた. HSC-3 細胞へのMHT (42°C, 90分) とHT (44°C, 90分) 負荷によりHSPであるHsp70, Hsp40とHsp27の顕著な発現誘導が観察された. 一方, これらの発現は, 正常と温熱負荷条件においてHSF1ノックダウン細胞で有意に減少した. HSC-3 細胞においてHSF1ノックダウンは, 37°Cにおける生存細胞数を抑制したので, HSF1は通常の細胞増殖に必要であると考えられる. また, MHTとHTの感受性は, HSF1ノックダウンHSC-3 細胞において顕著に増感された. さらに, HSF1ノックダウンによるこれらのHT感受性の増感効果は, 他のヒトOSCC であるHO-1-N-1, HO-1-u-1とSAS細胞で観察された. 以上より, HSF1の抑制はヒトOSCC細胞のMHT とHTに対する感受性を増感すること, さらに, HSF1の抑制とHTとの併用は, OSCCの有効な治療法になる可能性があることが示された.
AbstractList 様々ながんに対して, 抗がん効果が期待できるハイパーサーミア (HT) が適用されている. しかしながら, ヒートショックタンパク質 (HSP) の誘導によりがん細胞が温熱耐性能を獲得し, HT効果を減弱させることが知られている. 近年, ヒートショック転写因子1 (HSF1) の抑制が温熱感受性を上昇させることがいくつかの種類のがん細胞で示された. 本研究では, ヒト口腔扁平上皮がん (OSCC) 細胞においてマイルドハイパーサーミア (MHT) とHTの感受性に対する低分子干渉RNA (siRNA) を用いたHSF1の抑制の効果を検討した. ヒトOSCCであるHSC-3細胞へのsiHSF1-2 (HSF1に対するsiRNA) の添加は, 37°Cにおいて時間依存的にHSF1タンパク質レベルを抑制し, その添加48時間後でほぼ完全なHSF1のノックダウン効果を観察できた. HSC-3 細胞へのMHT (42°C, 90分) とHT (44°C, 90分) 負荷によりHSPであるHsp70, Hsp40とHsp27の顕著な発現誘導が観察された. 一方, これらの発現は, 正常と温熱負荷条件においてHSF1ノックダウン細胞で有意に減少した. HSC-3 細胞においてHSF1ノックダウンは, 37°Cにおける生存細胞数を抑制したので, HSF1は通常の細胞増殖に必要であると考えられる. また, MHTとHTの感受性は, HSF1ノックダウンHSC-3 細胞において顕著に増感された. さらに, HSF1ノックダウンによるこれらのHT感受性の増感効果は, 他のヒトOSCC であるHO-1-N-1, HO-1-u-1とSAS細胞で観察された. 以上より, HSF1の抑制はヒトOSCC細胞のMHT とHTに対する感受性を増感すること, さらに, HSF1の抑制とHTとの併用は, OSCCの有効な治療法になる可能性があることが示された.
Author 大塚, 健三
古澤, 之裕
田渕, 圭章
和田, 重人
近藤, 隆
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  fullname: 近藤, 隆
  organization: 富山大学大学院医薬学研究部・放射線基礎医学
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References 23) Nakamura Y., Fujimoto M., Hayashida N., Takii R., Nakai A., Muto M. : Silencing HSF1 by short hairpin RNA decreases cell proliferation and enhances sensitivity to hyperthermia in human melanoma cell lines. J Dermatol Sci, 60 : 187-192, 2010.
22) Rossi A., Ciafrè S., Balsamo M., Pierimarchi P., Santoro M.G. : Targeting the heat shock factor 1 by RNA interference : A potent tool to enhance hyperthermochemotherapy efficacy in cervical cancer. Cancer Res, 66 : 7678-7685, 2006.
32) Stanhill A., Levin V., Hendel A., Shachar I., Kazanov D., Arber N., Kaminski N., Engelberg D. : Ha-rasval12 induces HSP70b transcription via the HSE/HSF1 system, but HSP70b expression is suppressed in Ha-rasval12-transformed cells. Oncogene, 25 : 1485-1495, 2006.
17) Hoang A.T., Huang J., Rudra-Ganguly N., Zheng J., Powell W.C., Rabindran S.K., Wu C., Roy-Burman P. : A novel association between the human heat shock transcription factor 1 (HSF1) and prostate adenocarcinoma. Am J Pathol, 156 : 857-864, 2000.
33) Lee S.B., Huang K., Palmer R., Truong V.B., Herzlinger D., Kolquist K.A., Wong J., Paulding C., Yoon S.K., Gerald W., Oliner J.D., Haber D.A. : The Wilms tumor suppressor WT1 encodes a transcriptional activator of amphiregulin. Cell, 98 : 663-673, 1999.
34) Xiao N., DeFranco D.B. : Overexpression of unliganded steroid receptors activates endogenous heat shock factor. Mol Endocrinol, 11 : 1365-1374, 1997.
27) Towbin H., Staehelin T., Gordon J. : Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets : Procedure and some applications. Proc Natl Acad Sci USA, 76 : 4350-4354, 1979.
16) Tavassol F., Starke O.F., Kokemüller H., Wegener G., Müller-Tavassol C.C., Gellrich N.C., Eckardt A. : Prognostic significance of heat shock protein 70 (HSP70) in patients with oral cancer. Head Neck Oncol, 3 : 10, 2011.
18) Khaleque M.A., Bharti A., Gong J., Gray P.J., Sachdev V., Ciocca D.R., Stati A., Fanelli M., Calderwood S.K. : Heat shock factor 1 represses estrogen-dependent transcription through association with MTA1. Oncogene, 27 : 1886-1893, 2008.
29) Kameda T., Ikegami K., Liu Y., Terada K., Sugiyama T. : A hypothermic-temperature-sensitive gene silencing by the mammalian RNAi. Biochem Biophys Res Commun, 315 : 599-602, 2004.
31) Cai L., Zhu J.D. : The tumor-selective over-expression of the human Hsp70 gene is attributed to the aberrant controls at both initiation and elongation levels of transcription. Cell Res, 13 : 93-109, 2003.
28) Fortier E., Belote J.M. : Temperature-dependent gene silencing by an expressed inverted repeat in Drosophila . Genesis, 26 : 240-244, 2000.
1) Scully C., Bagan J.V. : Recent advances in oral oncology 2008 ; squamous cell carcinoma imaging, treatment, prognostication and treatment outcomes. Oral Oncol, 45 : e25-e30, 2009.
24) Zhang Y., Huang L., Zhang J., Moskophidis D., Mivechi N.F. : Targeted disruption of hsf1 leads to lack of thermotolerance and defines tissue-specific regulation for stress-inducible Hsp molecular chaperones. J Cell Biochem, 86 : 376-393, 2002.
7) Li G.C., Mivechi N.F., Weitzel G. : Heat shock proteins, thermotolerance, and their relevance to clinical hyperthermia. Int J Hyperthermia, 11 : 459-488, 1995.
6) Tohnai I., Hayashi Y., Mitsudo K., Shigetomi T., Ueda M., Ishigaki T. : Prognostic evaluation of preoperative thermochemoradiotherapy for N3 cervical lymph node metastases of oral cancer. Oncology, 62 : 234-240, 2002.
2) Cruz J.J., Ocaña A., Navarro M., Barco E.D., Fonseca E. : New options in the treatment of locally advanced head and neck cancer : Role for induction chemotherapy. Cancer Treat Rev, 34 : 268-274, 2008.
3) Wust P., Hildebrandt B., Sreenivasa G., Rau B., Gellermann J., Riess H., Felix R., Schlag P.M. : Hyperthermia in combined treatment of cancer. Lancet Oncol, 3 : 487-497, 2002.
15) Tang D., Khaleque M.A., Jones E.L., Theriault J.R., Li C., Wong W.H., Stevenson M.A., Calderwood S.K. : Expression of heat shock proteins and heat shock protein messenger ribonucleic acid in human prostate carcinoma in vitro and in tumors in vivo . Cell Stress Chaperones, 10 : 46-58, 2005.
21) Wang J.H., Yao M.Z., Gu J.F., Sun L.Y., Shen Y.F., Liu X.Y. : Blocking HSF1 by dominant-negative mutant to sensitize tumor cells to hyperthermia. Biochem Biophys Res Commun, 290 : 1454-1461, 2002.
35) Lu S., Tan Z., Wortman M., Lu S., Dong Z. : Regulation of heat shock protein 70-1 expression by androgen receptor and its signaling in human prostate cancer cells. Int J Oncol, 36 : 459-467, 2010.
13) Akerfelt M., Morimoto R.I., Sistonen L. : Heat shock factors : Integrators of cell stress, development and lifespan. Nat Rev Mol Cell Biol, 11 : 545-555, 2010.
10) Georgopoulos C., Welch W.J. : Role of the major heat shock proteins as molecular chaperones. Annu Rev Cell Biol, 9 : 601-634, 1993.
14) Nakajima M., Kuwano H., Miyazaki T., Masuda N., Kato H. : Significant correlation between expression of heat shock proteins 27, 70 and lymphocyte infiltration in esophageal squamous cell carcinoma. Cancer Lett, 178 : 99-106, 2002.
30) Dai C., Whitesell L., Rogers A.B., Lindquist S. : Heat shock factor 1 is a powerful multifaceted modifier of carcinogenesis. Cell, 130 : 1005-1018, 2007.
12) Morimoto R.I. : Regulation of the heat shock transcriptional response : Cross talk between a family of heat shock factors, molecular chaperones, and negative regulators. Genes Dev, 12 : 3788-3796, 1998.
26) Laemmli U.K. : Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, 227 : 680-685, 1970.
11) Beere H.M. : "The stress of dying" : The role of heat shock proteins in the regulation of apoptosis. J Cell Sci, 117 : 2641-2651, 2004.
5) Wust P., Stahl H., Dieckmann K., Scheller S., Löffel J., Riess H., Bier J., Jahnke V., Felix R. : Local hyperthermia of N2/N3 cervical lymph node metastases : Correlation of technical/thermal parameters and response. Int J Radiat Oncol Biol Phys, 34 : 635-646, 1996.
19) Ishiwata J., Kasamatsu A., Sakuma K., Iyoda M., Yamatoji M., Usukura K., Ishige S., Shimizu T., Yamano Y., Ogawara K., Shiiba M., Tanzawa H., Uzawa K. : State of heat shock factor 1 expression as a putative diagnostic marker for oral squamous cell carcinoma. Int J Oncol, 40 : 47-52, 2012.
20) Santagata S., Hu R., Lin N.U., Mendillo M.L., Collins L.C., Hankinson S.E., Schnitt S.J., Whitesell L., Tamimi R.M., Lindquist S., Ince T.A. : High levels of nuclear heat-shock factor 1 (HSF1) are associated with poor prognosis in breast cancer. Proc Natl Acad Sci USA, 108 : 18378-18383, 2011.
9) Lindquist S., Craig E.A. : The heat-shock proteins. Annu Rev Genet, 22 : 631-677, 1988.
4) Huilgol N.G., Gupta S., Dixit R. : Chemoradiation with hyperthermia in the treatment of head and neck cancer. Int J Hyperthermia, 26 : 21-25, 2010.
25) Kameda K., Kondo T., Tanabe K., Zhao Q.L., Seto H. : The role of intracellular Ca2+ in apoptosis induced by hyperthermia and its enhancement by verapamil in U937 cells. Int J Radiat Oncol Biol Phys, 49 : 1369-1379, 2001.
8) Mosser D.D., Morimoto R.I. : Molecular chaperones and the stress of oncogenesis. Oncogene, 23 : 2907-2918, 2004.
References_xml – reference: 27) Towbin H., Staehelin T., Gordon J. : Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets : Procedure and some applications. Proc Natl Acad Sci USA, 76 : 4350-4354, 1979.
– reference: 2) Cruz J.J., Ocaña A., Navarro M., Barco E.D., Fonseca E. : New options in the treatment of locally advanced head and neck cancer : Role for induction chemotherapy. Cancer Treat Rev, 34 : 268-274, 2008.
– reference: 5) Wust P., Stahl H., Dieckmann K., Scheller S., Löffel J., Riess H., Bier J., Jahnke V., Felix R. : Local hyperthermia of N2/N3 cervical lymph node metastases : Correlation of technical/thermal parameters and response. Int J Radiat Oncol Biol Phys, 34 : 635-646, 1996.
– reference: 31) Cai L., Zhu J.D. : The tumor-selective over-expression of the human Hsp70 gene is attributed to the aberrant controls at both initiation and elongation levels of transcription. Cell Res, 13 : 93-109, 2003.
– reference: 26) Laemmli U.K. : Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, 227 : 680-685, 1970.
– reference: 6) Tohnai I., Hayashi Y., Mitsudo K., Shigetomi T., Ueda M., Ishigaki T. : Prognostic evaluation of preoperative thermochemoradiotherapy for N3 cervical lymph node metastases of oral cancer. Oncology, 62 : 234-240, 2002.
– reference: 28) Fortier E., Belote J.M. : Temperature-dependent gene silencing by an expressed inverted repeat in Drosophila . Genesis, 26 : 240-244, 2000.
– reference: 32) Stanhill A., Levin V., Hendel A., Shachar I., Kazanov D., Arber N., Kaminski N., Engelberg D. : Ha-rasval12 induces HSP70b transcription via the HSE/HSF1 system, but HSP70b expression is suppressed in Ha-rasval12-transformed cells. Oncogene, 25 : 1485-1495, 2006.
– reference: 18) Khaleque M.A., Bharti A., Gong J., Gray P.J., Sachdev V., Ciocca D.R., Stati A., Fanelli M., Calderwood S.K. : Heat shock factor 1 represses estrogen-dependent transcription through association with MTA1. Oncogene, 27 : 1886-1893, 2008.
– reference: 8) Mosser D.D., Morimoto R.I. : Molecular chaperones and the stress of oncogenesis. Oncogene, 23 : 2907-2918, 2004.
– reference: 23) Nakamura Y., Fujimoto M., Hayashida N., Takii R., Nakai A., Muto M. : Silencing HSF1 by short hairpin RNA decreases cell proliferation and enhances sensitivity to hyperthermia in human melanoma cell lines. J Dermatol Sci, 60 : 187-192, 2010.
– reference: 13) Akerfelt M., Morimoto R.I., Sistonen L. : Heat shock factors : Integrators of cell stress, development and lifespan. Nat Rev Mol Cell Biol, 11 : 545-555, 2010.
– reference: 16) Tavassol F., Starke O.F., Kokemüller H., Wegener G., Müller-Tavassol C.C., Gellrich N.C., Eckardt A. : Prognostic significance of heat shock protein 70 (HSP70) in patients with oral cancer. Head Neck Oncol, 3 : 10, 2011.
– reference: 12) Morimoto R.I. : Regulation of the heat shock transcriptional response : Cross talk between a family of heat shock factors, molecular chaperones, and negative regulators. Genes Dev, 12 : 3788-3796, 1998.
– reference: 19) Ishiwata J., Kasamatsu A., Sakuma K., Iyoda M., Yamatoji M., Usukura K., Ishige S., Shimizu T., Yamano Y., Ogawara K., Shiiba M., Tanzawa H., Uzawa K. : State of heat shock factor 1 expression as a putative diagnostic marker for oral squamous cell carcinoma. Int J Oncol, 40 : 47-52, 2012.
– reference: 33) Lee S.B., Huang K., Palmer R., Truong V.B., Herzlinger D., Kolquist K.A., Wong J., Paulding C., Yoon S.K., Gerald W., Oliner J.D., Haber D.A. : The Wilms tumor suppressor WT1 encodes a transcriptional activator of amphiregulin. Cell, 98 : 663-673, 1999.
– reference: 10) Georgopoulos C., Welch W.J. : Role of the major heat shock proteins as molecular chaperones. Annu Rev Cell Biol, 9 : 601-634, 1993.
– reference: 30) Dai C., Whitesell L., Rogers A.B., Lindquist S. : Heat shock factor 1 is a powerful multifaceted modifier of carcinogenesis. Cell, 130 : 1005-1018, 2007.
– reference: 4) Huilgol N.G., Gupta S., Dixit R. : Chemoradiation with hyperthermia in the treatment of head and neck cancer. Int J Hyperthermia, 26 : 21-25, 2010.
– reference: 22) Rossi A., Ciafrè S., Balsamo M., Pierimarchi P., Santoro M.G. : Targeting the heat shock factor 1 by RNA interference : A potent tool to enhance hyperthermochemotherapy efficacy in cervical cancer. Cancer Res, 66 : 7678-7685, 2006.
– reference: 9) Lindquist S., Craig E.A. : The heat-shock proteins. Annu Rev Genet, 22 : 631-677, 1988.
– reference: 11) Beere H.M. : "The stress of dying" : The role of heat shock proteins in the regulation of apoptosis. J Cell Sci, 117 : 2641-2651, 2004.
– reference: 15) Tang D., Khaleque M.A., Jones E.L., Theriault J.R., Li C., Wong W.H., Stevenson M.A., Calderwood S.K. : Expression of heat shock proteins and heat shock protein messenger ribonucleic acid in human prostate carcinoma in vitro and in tumors in vivo . Cell Stress Chaperones, 10 : 46-58, 2005.
– reference: 29) Kameda T., Ikegami K., Liu Y., Terada K., Sugiyama T. : A hypothermic-temperature-sensitive gene silencing by the mammalian RNAi. Biochem Biophys Res Commun, 315 : 599-602, 2004.
– reference: 24) Zhang Y., Huang L., Zhang J., Moskophidis D., Mivechi N.F. : Targeted disruption of hsf1 leads to lack of thermotolerance and defines tissue-specific regulation for stress-inducible Hsp molecular chaperones. J Cell Biochem, 86 : 376-393, 2002.
– reference: 25) Kameda K., Kondo T., Tanabe K., Zhao Q.L., Seto H. : The role of intracellular Ca2+ in apoptosis induced by hyperthermia and its enhancement by verapamil in U937 cells. Int J Radiat Oncol Biol Phys, 49 : 1369-1379, 2001.
– reference: 7) Li G.C., Mivechi N.F., Weitzel G. : Heat shock proteins, thermotolerance, and their relevance to clinical hyperthermia. Int J Hyperthermia, 11 : 459-488, 1995.
– reference: 17) Hoang A.T., Huang J., Rudra-Ganguly N., Zheng J., Powell W.C., Rabindran S.K., Wu C., Roy-Burman P. : A novel association between the human heat shock transcription factor 1 (HSF1) and prostate adenocarcinoma. Am J Pathol, 156 : 857-864, 2000.
– reference: 20) Santagata S., Hu R., Lin N.U., Mendillo M.L., Collins L.C., Hankinson S.E., Schnitt S.J., Whitesell L., Tamimi R.M., Lindquist S., Ince T.A. : High levels of nuclear heat-shock factor 1 (HSF1) are associated with poor prognosis in breast cancer. Proc Natl Acad Sci USA, 108 : 18378-18383, 2011.
– reference: 34) Xiao N., DeFranco D.B. : Overexpression of unliganded steroid receptors activates endogenous heat shock factor. Mol Endocrinol, 11 : 1365-1374, 1997.
– reference: 3) Wust P., Hildebrandt B., Sreenivasa G., Rau B., Gellermann J., Riess H., Felix R., Schlag P.M. : Hyperthermia in combined treatment of cancer. Lancet Oncol, 3 : 487-497, 2002.
– reference: 14) Nakajima M., Kuwano H., Miyazaki T., Masuda N., Kato H. : Significant correlation between expression of heat shock proteins 27, 70 and lymphocyte infiltration in esophageal squamous cell carcinoma. Cancer Lett, 178 : 99-106, 2002.
– reference: 1) Scully C., Bagan J.V. : Recent advances in oral oncology 2008 ; squamous cell carcinoma imaging, treatment, prognostication and treatment outcomes. Oral Oncol, 45 : e25-e30, 2009.
– reference: 21) Wang J.H., Yao M.Z., Gu J.F., Sun L.Y., Shen Y.F., Liu X.Y. : Blocking HSF1 by dominant-negative mutant to sensitize tumor cells to hyperthermia. Biochem Biophys Res Commun, 290 : 1454-1461, 2002.
– reference: 35) Lu S., Tan Z., Wortman M., Lu S., Dong Z. : Regulation of heat shock protein 70-1 expression by androgen receptor and its signaling in human prostate cancer cells. Int J Oncol, 36 : 459-467, 2010.
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Snippet 様々ながんに対して, 抗がん効果が期待できるハイパーサーミア (HT) が適用されている. しかしながら, ヒートショックタンパク質 (HSP) の誘導によりがん細胞が温熱耐性能を獲得し, HT効果を減弱させることが知られている. 近年, ヒートショック転写因子1 (HSF1)...
SourceID jstage
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StartPage 99
Title ヒト口腔扁平上皮がん細胞において低分子干渉RNAを用いたヒートショック転写因子1の抑制はマイルドハイパーサーミアとハイパーサーミアの感受性を増感する
URI https://www.jstage.jst.go.jp/article/thermalmed/27/4/27_4_99/_article/-char/ja
Volume 27
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ispartofPNX Thermal Medicine, 2011/12/20, Vol.27(4), pp.99-108
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