肝由来培養細胞の増殖に及ぼす副腎皮質ホルモンの影響
各種培養細胞を用い,細胞増殖に及ぼす各種副腎皮質ホルモンの影響を観察した.各種副腎皮質ホルモンは,成熟ラット肝から分離した上皮様細胞を除くと,既存の株化線維芽細胞(3T3, NRK, L),ヒト肝由来の株化肝細胞(Chang)および成熟ラット肝から分離した線維芽細胞に対し,いずれも細胞増殖抑制的に作用し,その作用は0.05～50μg/mlの濃度ではdosis responseが認められた.この作用の強さは細胞種間および副腎皮質ホルモン製剤間でそれぞれ差がみられ,ヒト肝由来のChang細胞は最も感受性が高く,またBetamethasone, Prednisolone,Hydrocortisone...
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| Published in | 肝臓 Vol. 19; no. 7; pp. 627 - 632 |
|---|---|
| Main Authors | , , , |
| Format | Journal Article |
| Language | Japanese |
| Published |
一般社団法人 日本肝臓学会
01.07.1978
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| Subjects | |
| Online Access | Get full text |
| ISSN | 0451-4203 1881-3593 |
| DOI | 10.2957/kanzo.19.627 |
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| Abstract | 各種培養細胞を用い,細胞増殖に及ぼす各種副腎皮質ホルモンの影響を観察した.各種副腎皮質ホルモンは,成熟ラット肝から分離した上皮様細胞を除くと,既存の株化線維芽細胞(3T3, NRK, L),ヒト肝由来の株化肝細胞(Chang)および成熟ラット肝から分離した線維芽細胞に対し,いずれも細胞増殖抑制的に作用し,その作用は0.05～50μg/mlの濃度ではdosis responseが認められた.この作用の強さは細胞種間および副腎皮質ホルモン製剤間でそれぞれ差がみられ,ヒト肝由来のChang細胞は最も感受性が高く,またBetamethasone, Prednisolone,Hydrocortisoneの順に作用が強く発現した.線維芽細胞に対しては比較的作用が弱く,その強さも逆の順であるかの如くであった.以上の成績から,肝疾患に対する副腎皮質ホルモン投与に際しては,肝細胞に対する副腎皮質ホルモンの細胞増殖抑制機序ならびに製剤間にみられる作用の強さの差なども考慮すべきことを考察した. |
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| AbstractList | 各種培養細胞を用い,細胞増殖に及ぼす各種副腎皮質ホルモンの影響を観察した.各種副腎皮質ホルモンは,成熟ラット肝から分離した上皮様細胞を除くと,既存の株化線維芽細胞(3T3, NRK, L),ヒト肝由来の株化肝細胞(Chang)および成熟ラット肝から分離した線維芽細胞に対し,いずれも細胞増殖抑制的に作用し,その作用は0.05～50μg/mlの濃度ではdosis responseが認められた.この作用の強さは細胞種間および副腎皮質ホルモン製剤間でそれぞれ差がみられ,ヒト肝由来のChang細胞は最も感受性が高く,またBetamethasone, Prednisolone,Hydrocortisoneの順に作用が強く発現した.線維芽細胞に対しては比較的作用が弱く,その強さも逆の順であるかの如くであった.以上の成績から,肝疾患に対する副腎皮質ホルモン投与に際しては,肝細胞に対する副腎皮質ホルモンの細胞増殖抑制機序ならびに製剤間にみられる作用の強さの差なども考慮すべきことを考察した. |
| Author | 島村, 真里子 片山, 敬 三浦, 幸子 木村, 恒夫 |
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| DOI | 10.2957/kanzo.19.627 |
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| References | 8) Moscatelli, D. & Rubin, H.: Hormonal control of hyaluronic acid production in fibroblasts and its relation to nucreic acid and protein synthesis. J. Cell Physiol., 91: 79-88, 1977. 12) Grove, G.L. & Cristofaro, V.J.: Characterization of the cell cycle of cultured human diploid cells: Effects of aging and hydocortisone. J. Cell Physiol., 90: 415-422, 1976. 7) Richman, R.A.: Hormonal stimulation of DNA synthesis in primary cultures of adult rat hepatocytes. Proc. Nat. Acad. Sci., 73: 3589-3593, 1976. 11) DeAsua, L.J.: Temporal sequence of hormonal interactions during the prereplicative phase of quiscent cultured 3T3 fibroblasts. Proc. Nat. Acad. Sci., 74: 3845-3849, 1977. 10) Loeb, J.N. & Sternschein, M.J.: Suppression of thymidine incorporation into the gastric mucosa of cortisone-treated rats: Possible relation to glucocorticoid-induced gastric ulceration. Endocrinology, 92: 1322-1327, 1973. 6) Grasso, R.J.: Transient inhibition of cell proliferation in rat glioma monolayer cultures by cortisol. Cancer Res., 36: 2408-2414, 1976. 9) Rubin, H.: Antagonistic effects of insulin and cortisol on coordinate control of metabolism and growth in cultured fibroblasts. J. Cell Physiol., 91: 249-260, 1977. 13) Kollmorgen, G.M. & Griffin, M.J.: The effect of hydrocortisone on Hela cell growth. Cell Tissure Kinet., 2: 111-122, 1969. 1) Howard, E.: Effects of corticosteroid and food restriction on growth and on DNA, RNA and cholesterol contents of the brain and liver in infant mice. J. Neurochem., 12: 181-191, 1965. 2) Ruhmann, A.G. & Berliner, D.L.: Effect of steroids on growth of mouse fibroblasts in vitro. Endocrinology, 76: 916-927, 1965. 4) Henderson, I.C. & Loeb, J.N.: Fall in liver DNA polymerase activity in cortisone-treated rats. Nature, 228: 556-557, 1970. 5) Fodge, D. & Rubin, H.: Differential effects of glucocorticoids on DNA sythesis in normal and virus-transformed chicken emblyo cells. Nature, 257: 804-806, 1975. 3) Pratt, W.B. & Aronow, L.: The effect of glucocorticoids on protein and nucreic acid synthesis in mouse fibroblasts growing in vitro. J. Biol. Chem., 241: 5244-5250, 1966. 14) Smith, B.T.: The growth promoting effect of cortisol on human fetal lung cells. Steroids, 22: 515-523, 1973. |
| References_xml | – reference: 4) Henderson, I.C. & Loeb, J.N.: Fall in liver DNA polymerase activity in cortisone-treated rats. Nature, 228: 556-557, 1970. – reference: 10) Loeb, J.N. & Sternschein, M.J.: Suppression of thymidine incorporation into the gastric mucosa of cortisone-treated rats: Possible relation to glucocorticoid-induced gastric ulceration. Endocrinology, 92: 1322-1327, 1973. – reference: 7) Richman, R.A.: Hormonal stimulation of DNA synthesis in primary cultures of adult rat hepatocytes. Proc. Nat. Acad. Sci., 73: 3589-3593, 1976. – reference: 1) Howard, E.: Effects of corticosteroid and food restriction on growth and on DNA, RNA and cholesterol contents of the brain and liver in infant mice. J. Neurochem., 12: 181-191, 1965. – reference: 5) Fodge, D. & Rubin, H.: Differential effects of glucocorticoids on DNA sythesis in normal and virus-transformed chicken emblyo cells. Nature, 257: 804-806, 1975. – reference: 11) DeAsua, L.J.: Temporal sequence of hormonal interactions during the prereplicative phase of quiscent cultured 3T3 fibroblasts. Proc. Nat. Acad. Sci., 74: 3845-3849, 1977. – reference: 13) Kollmorgen, G.M. & Griffin, M.J.: The effect of hydrocortisone on Hela cell growth. Cell Tissure Kinet., 2: 111-122, 1969. – reference: 8) Moscatelli, D. & Rubin, H.: Hormonal control of hyaluronic acid production in fibroblasts and its relation to nucreic acid and protein synthesis. J. Cell Physiol., 91: 79-88, 1977. – reference: 12) Grove, G.L. & Cristofaro, V.J.: Characterization of the cell cycle of cultured human diploid cells: Effects of aging and hydocortisone. J. Cell Physiol., 90: 415-422, 1976. – reference: 2) Ruhmann, A.G. & Berliner, D.L.: Effect of steroids on growth of mouse fibroblasts in vitro. Endocrinology, 76: 916-927, 1965. – reference: 3) Pratt, W.B. & Aronow, L.: The effect of glucocorticoids on protein and nucreic acid synthesis in mouse fibroblasts growing in vitro. J. Biol. Chem., 241: 5244-5250, 1966. – reference: 9) Rubin, H.: Antagonistic effects of insulin and cortisol on coordinate control of metabolism and growth in cultured fibroblasts. J. Cell Physiol., 91: 249-260, 1977. – reference: 14) Smith, B.T.: The growth promoting effect of cortisol on human fetal lung cells. Steroids, 22: 515-523, 1973. – reference: 6) Grasso, R.J.: Transient inhibition of cell proliferation in rat glioma monolayer cultures by cortisol. Cancer Res., 36: 2408-2414, 1976. |
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| Snippet | 各種培養細胞を用い,細胞増殖に及ぼす各種副腎皮質ホルモンの影響を観察した.各種副腎皮質ホルモンは,成熟ラット肝から分離した上皮様細胞を除くと,既存の株化線維芽細胞(3T3, NRK,... |
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| SubjectTerms | 副腎皮質ホルモン 培養細胞(肝由来-) 慢性肝炎 細胞増殖抑制作用 |
| Title | 肝由来培養細胞の増殖に及ぼす副腎皮質ホルモンの影響 |
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