利用状況の異なる水浴プールにおける健康関連微生物の存在実態

集団感染症が報告されていない平常時において, 小学校やスポーツクラブなどのプールを対象に糞便汚染指標細菌・ウイルス, 腸管系ウイルスやレジオネラ属菌の検出を試み, 微生物汚染の実態調査と汚染要因の検証, 汚染指標の有効性評価を行った。利用状況等の条件によっては指標微生物の検出率が高くなった。大腸菌, F特異大腸菌ファージは培養法で検出され, 現行の管理手法では完全な不活化は困難だと考えられた。Pepper mild mottle virus, cross-assembly phageや, ヒト特異的なバクテロイデス属菌の遺伝子マーカーであるHF183は高頻度で検出され, 特にcross-ass...

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Published in水環境学会誌 Vol. 46; no. 5; pp. 113 - 122
Main Authors 明地, 柚乃, 高松, 由樹, 端, 昭彦, 石黒, 伶奈, 黒田, 啓介
Format Journal Article
LanguageJapanese
Published 公益社団法人 日本水環境学会 2023
Subjects
Pepper mild mottle virus
スイミングプール
レジオネラ
指標微生物
糞便汚染
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Abstract 集団感染症が報告されていない平常時において, 小学校やスポーツクラブなどのプールを対象に糞便汚染指標細菌・ウイルス, 腸管系ウイルスやレジオネラ属菌の検出を試み, 微生物汚染の実態調査と汚染要因の検証, 汚染指標の有効性評価を行った。利用状況等の条件によっては指標微生物の検出率が高くなった。大腸菌, F特異大腸菌ファージは培養法で検出され, 現行の管理手法では完全な不活化は困難だと考えられた。Pepper mild mottle virus, cross-assembly phageや, ヒト特異的なバクテロイデス属菌の遺伝子マーカーであるHF183は高頻度で検出され, 特にcross-assembly phage, HF183は水質管理下のプールにおいてもヒト糞便汚染指標としての有効性が示唆された。腸管系ウイルスは不検出であったが, 感染者の利用により, 感染力を保持したウイルスが滞留する可能性が示された。
AbstractList 集団感染症が報告されていない平常時において, 小学校やスポーツクラブなどのプールを対象に糞便汚染指標細菌・ウイルス, 腸管系ウイルスやレジオネラ属菌の検出を試み, 微生物汚染の実態調査と汚染要因の検証, 汚染指標の有効性評価を行った。利用状況等の条件によっては指標微生物の検出率が高くなった。大腸菌, F特異大腸菌ファージは培養法で検出され, 現行の管理手法では完全な不活化は困難だと考えられた。Pepper mild mottle virus, cross-assembly phageや, ヒト特異的なバクテロイデス属菌の遺伝子マーカーであるHF183は高頻度で検出され, 特にcross-assembly phage, HF183は水質管理下のプールにおいてもヒト糞便汚染指標としての有効性が示唆された。腸管系ウイルスは不検出であったが, 感染者の利用により, 感染力を保持したウイルスが滞留する可能性が示された。
Author 端, 昭彦
明地, 柚乃
黒田, 啓介
高松, 由樹
石黒, 伶奈
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References_xml – reference: 14) 赤井仁志, 紀谷文樹, 野知啓子, 松村佳明, 松鵜さとみ, 福島雅弘, 佐藤隆, 野崎英之, 2015. 水泳プール・浴槽用珪藻土式ろ過器の性能評価のための基本事項の整理と検討. 空気調和・衛生工学会大会学術講演論文集, pp.157-160.
– reference: 16) Podewils, L.J., Zanardi Blevins, L., Hagenbuch, M., Itani, D., Burns, A., Otto, C., Blanton, L., Adams, S., Monroe, S.S., Beach, M.J., Widdowson, M., 2007. Outbreak of norovirus illness associated with a swimming pool. Epidemiology & Infection 135 (5) , 827-833.
– reference: 37) de Roda Husman, A.M., Lodder, W.J., Rutjes, S.A., Schijven, J.F., Teunis, P.F.M., 2009. Long-term inactivation study of three enteroviruses in artificial surface and groundwaters, using PCR and cell culture. Applied and Environmental Microbiology 75 (4) , 1050-1057.
– reference: 41) 厚生労働省, 2019. 循環式浴槽におけるレジオネラ症防止対策マニュアル. URL. https://www.mhlw.go.jp/content/11130500/000577571.pdf (2022年8月時点) .
– reference: 32) Hata, A., Katayama, H., Kitajima, M., Visvanathan, C., Nol, C., Furumai, H., 2011. Validation of internal controls for extraction and amplification of nucleic acids from enteric viruses in water samples. Applied and Environmental Microbiology 77 (13) , 4336-4343.
– reference: 40) Donohue, M.J., Vesper, S., Mistry, J., Donohue, J.M., 2019. Impact of chlorine and chloramine on the detection and quantification of Legionella pneumophila and Mycobacterium species. Applied and Environmental Microbiology 85 (24) , e01942-19.
– reference: 23) Bernasconi, C., Volponi, G., Picozzi, C., Foschino, R., 2007. Use of the tna operon as a new molecular target for Escherichia coli detection. Applied and Environmental Microbiology 73 (19) , 6321-6325.
– reference: 29) Haramoto, E., Kitajima, M., Kishida, N., Konno, Y., Katayama, H., Asami, M., Akiba, M., 2013. Occurrence of pepper mild mottle virus in drinking water sources in Japan. Applied and Environmental Microbiology 79 (23) , 7413-7418.
– reference: 11) 国立感染症研究所, 2014. レジオネラ症とは. URL. https://www.niid.go.jp/niid/ja/diseases/ra/legionella/392-encyclopedia/530-legionella.html (2022年8月時点) .
– reference: 17) Liu, M., Hata, A., Katayama, H., Kasuga, I., 2020. Consecutive ultrafiltration and silica adsorption for recovery of extracellular antibiotic resistance genes from an urban river. Environmental Pollution 260, 114062.
– reference: 43) Hata, A., Meuchi, Y., Liu, M., Torii, S., Katayama, H., 2023. Surfactant treatment for efficient gene detection of enteric viruses and indicators in surface water concentrated by ultrafiltration. Food and Environmental Virology 15 (1) , 8-20.
– reference: 10) 国立感染症研究所, 2021. レジオネラ症の届出状況, 2011年第1週～2021年第35週. URL. https://www.niid.go.jp/niid/ja/legionella-m/legionella-idwrs.html (2022年8月時点) .
– reference: 38) Calero-Cáceres, W., Muniesa, M., 2016. Persistence of naturally occurring antibiotic resistance genes in the bacteria and bacteriophage fractions of wastewater. Water Research 95, 11-18.
– reference: 20) Hata, A., Hanamoto, S., Shirasaka, Y., Yamashita, N., Tanaka, H., 2016. Quantitative distribution of infectious F-specific RNA phage genotypes in surface waters. Applied and Environmental Microbiology 82 (14) , 4244-4252.
– reference: 30) Kageyama, T., Kojima, S., Shinohara, M., Uchida, K., Fukushi, S., Hoshino, F.B., Takeda, N., Katayama, K., 2003. Broadly reactive and highly sensitive assay for norwalk-like viruses based on real-time quantitative reverse transcription-PCR. Journal of Clinical Microbiology 41 (4) , 1548-1557.
– reference: 3) Sinclair, R.G., Jones, E.L., Gerba, C.P., 2009. Viruses in recreational water-borne disease outbreaks: A review. Journal of Applied Microbiology 107 (6) , 1769-1780.
– reference: 27) Diederen, B.M.W., de Jong, C.M.A., Marmouk, F., Kluytmans, J.A.J.W., Peeters, M.F., Van der Zee, A., 2007. Evaluation of real-time PCR for the early detection of Legionella pneumophila DNA in serum samples. Journal of Medical Microbiology 56 (1) , 94-101.
– reference: 22) Verreault, D., Rousseau, G.M., Gendron, L., Massé, D., Moineau, S., Duchaine, C., 2010. Comparison of polycarbonate and polytetrafluoroethylene filters for sampling of airborne bacteriophages. Aerosol Science and Technology 44 (3) , 197-201.
– reference: 15) Wei, X., Li, J., Hou, S., Xu, C., Zhang, H., Atwill, E.R., Li, X., Yang, Z., Chen, S., 2018. Assessment of microbiological safety of water in public swimming pools in Guangzhou, China. International Journal of Environmental Research and Public Health 15 (7) , 1416.
– reference: 12) 名古屋市, 2022. 報道資料令和4年6月2日発表入浴施設を原因とするレジオネラ症発生について. URL. https://www.city.nagoya.jp/kenkofukushi/page/0000153519.html (2022年6月時点) .
– reference: 34) Hata, A., Kitajima, M., Katayama, H., 2013. Occurrence and reduction of human viruses, F-specific RNA coliphage genogroups and microbial indicators at a full-scale wastewater treatment plant in Japan. Journal of Applied Microbiology 114 (2) , 545-554.
– reference: 1) 厚生労働省, 2007. 遊泳用プールの衛生基準について. URL. https://www.mhlw.go.jp/bunya/kenkou/seikatsu-eisei01/pdf/02a.pdf (2022年8月時点) .
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Snippet 集団感染症が報告されていない平常時において, 小学校やスポーツクラブなどのプールを対象に糞便汚染指標細菌・ウイルス, 腸管系ウイルスやレジオネラ属菌の検出を試み, 微生物汚染の実態調査と汚染要因の検証, 汚染指標の有効性評価を行った。利用状況等の条件によっては指標微生物の検出率が高くなった。大腸菌,...
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SourceType Publisher
StartPage 113
SubjectTerms Pepper mild mottle virus
スイミングプール
レジオネラ
指標微生物
糞便汚染
Title 利用状況の異なる水浴プールにおける健康関連微生物の存在実態
URI https://www.jstage.jst.go.jp/article/jswe/46/5/46_113/_article/-char/ja
Volume 46
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