Generation of high performance anti-hapten monoclonal antibodies and their application to trace characterization

• Published in: Japanese Journal of Clinical Chemistry Vol. 34; no. 2; pp. 125 - 145
• Main Authors: Kato, Yoshinori, Matsuki, Yasuhiko, Goto, Junichi, Kobayashi, Norihiro, Shimada, Kazutake
• Format: Journal Article
• Language: Japanese
• Published: Japan Society of Clinical Chemistry 2005; 一般社団法人 日本臨床化学会
• Subjects: bile acid; corticosteroid; dioxin; hapten; immunoaffinity extraction; immunoassay; monoclonal antibody; singlechain Fv fragment; steroid; variable region gene; vitamin D; イムノアッセイ; イムノアフィニティー抽出; コルチコイド; ステロイド; ダイオキシン; ハプテン; ビタミンD; モノクローナル抗体; 一本鎖FVフラグメント; 可変部遺伝子; 胆汁酸

The trace characterization of physiologically active substances with a low molecular weight (e. g., steroids, catecholamines and prostaglandins) is an important subject in clinical analysis, and specific a ntibodies against such“haptens” work as an essential analytical reagent. However, conventional...