IN VITRO PERFUSION CULTURE SYSTEM TO EVALUATE THE ENDOTHELIAL INJURY WITH BALLOON CATHETER

• Published in: Jinko Zoki Vol. 27; no. 2; pp. 514 - 518
• Main Authors: SUGAHARA, M., OOKAWA, K., OHSHIMA, N.
• Format: Journal Article
• Language: Japanese
• Published: JAPANESE SOCIETY FOR ARTIFICIAL ORGANS 1998
• Subjects: balloon injury; compressive force; endothelial cells; percutaneous transluminal coronary angioplasty
• ISSN: 0300-0818; 1883-6097
• DOI: 10.11392/jsao1972.27.514

To evaluate the endothelial injury which is regarded as a major cause of restenosis after percutaneous transluminal coronary angioplasty (PTCA), a perfusion culture system was constructed. Porcine aortic endothelial cells were cultured on the inner surface of a glass tube (3.0 mm, i. d.), and the tube was placed in a closed perfusion circuit (120 mL/min, steady flow). Compressive force (ca. 2.5 kgf/cm2, 1 min).was applied to a monolayer of endothelial cells by inflating a PTCA balloon catheter introduced within the tube, and effects of compressive force on the viability of the endothelial cells were estimated. After the application of a compressive injury, the endothelial monolayer was observed continuously under a video microscope. Thereafter, the effects of conditions of catheter operation on the endothelial injury were studied. The endothelial monolayer exposed to compressive force was found to be injured immediately after the deflation of the catheter, and the number of the intact endothelial cells decreased below 10% of that before the catheter operation. During the observation period of 48 h after the deflation, no apparent growth or repair of endothelial monolayer was observed at the denuded area. While, the endothelial injury was considerably suppressed when the balloon was deflated slowly. This system is thought useful in improving PTCA technology.