Heparin-Ca-Ni Precipitation Method for Measuring Cholesterol in High-Density Lipoprotein A Provable Mechanism for Precipitation and Optimum Assay Condition

• Published in: Japanese Journal of Clinical Chemistry Vol. 9; no. 4; pp. 420 - 426
• Main Authors: KOIDE, ASAO, SAITO, MASAYUKI, OHTANI, HIDEKI, UENO, YOSHISUMI, UEHARA, KASUYUKI
• Format: Journal Article
• Language: English; Japanese
• Published: Japan Society of Clinical Chemistry 1980
• ISSN: 0370-5633; 2187-4077
• DOI: 10.14921/jscc1971b.9.4_420

Heparin-Ca precipitation method for measurement of cholesterol in high-density lipoprotein (HDL-C) has been improved for a good separation of HDL from low-density lipoprotein (LDL) and very-low-density lipoprotein (VLDL) in normo-and hyperlipemic sera by adding NiCl2 (1mM) to heparin-Ca reagent. We proved that this method can be used for the separation of HDL fraction from the other lipoproteins in a specimen of serum triglycerides (TG) concentration of about 1g/dl. The correlation between the results of dextran sulfate-Mg precipitation method and present method is very high (n=46, r=0.980) with regression line, y=1.05x+0.4. Precision (CV) % was found to be 2.3% (n=10) within run and 3.5% (n=20) run-to-run. Present method was applied to measure HDL-C in normo-and hyperlipemic sera in the routine clinical measurement.