スギ（Cryptomeria japonica）葉2段階温熱水パーコレーション抽出物の抗酸化活性

スギ（Cryptomeria japonica）の木材は，建材や家具，伝統工芸品などに広く使用される。しかし，スギの葉は，ほとんど使用されることなく，廃棄されてしまう。本研究では，水を溶媒とする2段階温熱水パーコレーションによる秋田スギ葉抽出物の生理活性をin vitro試験とヒト皮膚ケラチノサイトHaCaTを用いた細胞試験で評価した。スギ葉の抽出液は，in vitroの抗酸化活性及びポリフェノール量のいずれも180 ℃抽出液が優れていた。また，HaCaTを過酸化水素や紫外線に暴露して酸化ストレスを誘導した場合に，80 ℃抽出液でも抗酸化性が認められたが，180 ℃抽出液の方が優れた抗酸化性を...

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Published in木材学会誌 Vol. 69; no. 3; pp. 109 - 116
Main Authors 佐藤, 友紀, 進藤, 昌, 林, 潤一郎, 榊, 郁子, 工藤, 真二, 松田, 奈夕, 上原, 健二
Format Journal Article
LanguageJapanese
Published 一般社団法人 日本木材学会 25.07.2023
Online AccessGet full text
ISSN0021-4795
1880-7577
DOI10.2488/jwrs.69.109

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Abstract スギ（Cryptomeria japonica）の木材は，建材や家具，伝統工芸品などに広く使用される。しかし，スギの葉は，ほとんど使用されることなく，廃棄されてしまう。本研究では，水を溶媒とする2段階温熱水パーコレーションによる秋田スギ葉抽出物の生理活性をin vitro試験とヒト皮膚ケラチノサイトHaCaTを用いた細胞試験で評価した。スギ葉の抽出液は，in vitroの抗酸化活性及びポリフェノール量のいずれも180 ℃抽出液が優れていた。また，HaCaTを過酸化水素や紫外線に暴露して酸化ストレスを誘導した場合に，80 ℃抽出液でも抗酸化性が認められたが，180 ℃抽出液の方が優れた抗酸化性を示した。したがって，2段階温熱水パーコレーションにより，特に180 ℃熱水抽出液中に秋田スギ葉における抗酸化性に優れた成分を溶出できることが示唆された。
AbstractList スギ（Cryptomeria japonica）の木材は，建材や家具，伝統工芸品などに広く使用される。しかし，スギの葉は，ほとんど使用されることなく，廃棄されてしまう。本研究では，水を溶媒とする2段階温熱水パーコレーションによる秋田スギ葉抽出物の生理活性をin vitro試験とヒト皮膚ケラチノサイトHaCaTを用いた細胞試験で評価した。スギ葉の抽出液は，in vitroの抗酸化活性及びポリフェノール量のいずれも180 ℃抽出液が優れていた。また，HaCaTを過酸化水素や紫外線に暴露して酸化ストレスを誘導した場合に，80 ℃抽出液でも抗酸化性が認められたが，180 ℃抽出液の方が優れた抗酸化性を示した。したがって，2段階温熱水パーコレーションにより，特に180 ℃熱水抽出液中に秋田スギ葉における抗酸化性に優れた成分を溶出できることが示唆された。
Author 進藤, 昌
佐藤, 友紀
工藤, 真二
林, 潤一郎
上原, 健二
松田, 奈夕
榊, 郁子
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  fullname: 工藤, 真二
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  fullname: 松田, 奈夕
  organization: 秋田県総合食品研究センター
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References 20) Gao, X., Xu, D., Zhang, X., Zhao, H.: Protective effect of lemon peel polyphenols on oxidative stress-induced damage to human keratinocyte HaCaT cells through activation of the Nrf2/HO-1 signaling pathway. Front. Nutr. 7, 606776 (2021).
27) 上田裕人, 菅原進太郎, 松田 靖, 村田達郎, 黒田泰弘, 星 良和, 椛田聖孝, 井越敬司, 小野政輝, 安田 伸：2段階熱処理による熊本県産ヤーコン葉の抗酸化活性とフェノール成分に及ぼす影響. 東海大学先進生命科学研究所紀要 2, 29-34 (2018).
11) Yu, Y., Wu, H.: Understanding the primary liquid products of cellulose hydrolysis in hot-compressed water at various reaction temperatures. Energy Fuels 24 (3) , 1963-1971 (2010).
5) Cheng, S., Chua, M., Chang, E., Huang, C., Chen, W., Chang, S.: Variations in insecticidal activity and chemical compositions of leaf essential oils from Cryptomeria japonica at different ages. Bioresour. Technol. 100 (1) , 465-470 (2009).
12) Yuliansyah, A., Kumagai, S., Hirajima, T., Sasaki, K.: Hydrothermal treatment of oil palm biomass in batch and semi-flow reactors. Energy Procedia 158, 675-680 (2019).
14) Kumagai, S., Hayashi, N., Sasaki, T., Nakada, M., Shibata, M.: Fractionation and saccharification of cellulose and hemicellulose in rice hull by hot-compressed-water treatment with two-step heating. J. Japan Inst. Ener. 83, 776-781 (2004).
6) Cha, J., Jeong, M., Jeong, S., Moon, S., Kil, B., Yun, S., Lee, K., Song, Y.: Chemical composition and antimicrobial activity of the essential oil of Cryptomeria japonica. Phytother. Res. 21 (3) , 295-299 (2007).
19) 沖 智之：総ポリフェノールの定量法. “食品機能性評価マニュアル集第III集”, 日本食品 科学工学会, pp.1-7, http://fmric.or.jp/ffd/ffmanual/100753_oki.pdf 2021年4月2日参照.
8) Scapini, T., Dos Santos, M., Bonatto, C., Wancura, J., Mulinari, J., Camargo, A., Klanovicz, N., Zabot, G., Tres, M., Fongaro, G., Treichel, H.: Hydrothermal pretreatment of lignocellulosic biomass for hemicellulose recovery. Bioresour. Technol. 342, 126033 (2021).
30) Wang, S., Lai, W., Chu, F., Lin, C., Shen, S., Chang, S.: Essential oil from the leaves of Cryptomeria japonica acts as a silverfish (Lepisma saccharina) repellent and insecticide. J. Wood Sci. 52, 522-526 (2006).
4) Matsunaga, T., Hasegawa, C., Kawasuji, T., Suzuki, H., Saito, H., Sagioka, T., Takahashi, R., Tsukamoto, H., Morikawa, T., Akiyama, T.: Isolation of the antiulcer compound in essential oil from the leaves of Cryptomeria japonica. Biol. Pharm. Bull. 23 (5) , 595-598 (2000).
17) 佐藤友紀, 上原健二, 工藤真二, 榊, 郁子, 林, 潤一郎, 進藤, 昌：シイタケ (Lentinula edodes) 廃菌床2段階温熱水パーコレーション抽出液の抗酸化活性. 木材学会誌 68(1) , 26-35(2022).
22) Costa, A., Facchini, G., Pinheiro, A., Silva, M., Bonner, M., Arbiser, J., Eberlin, S.: Honokiol protects skin cells against inflammation, collagenolysis, apoptosis, and senescence caused by cigarette smoke damage. Int. J. Dermatol. 56 (7) , 754-761 (2017).
24) Teramoto, S., Ishikura, N.: The formation of catechin and procyanidins in cell suspension cultures of Cryptomeria japonica. Shokubutsu-gaku-zasshi 98, 171-179 (1985).
26) 熊谷 聡, 林 信行, 坂木 剛, 中田正夫, 柴田昌男：加圧熱水2段階昇温による籾殻中セルロース, ヘミセルロースの成分分離と糖化. 日本エネルギー学会誌 83(10) , 776-781 (2004).
15) Abdullah, R., Ueda, K., Saka, S.: Hydrothermal decomposition of various crystalline celluloses as treated by semi-flow hot-compressed water. J. Wood Sci. 60, 278-286 (2014).
28) Gunaseelan, S., Balupillai, A., Govindasamy, K., Ramasamy, K., Muthusamy, G., Shanmugam, M., Thangaiyan, R., Robert, B., Nagarajan, R., Ponniresan, V., Rathinaraj, P.: Linalool prevents oxidative stress activated protein kinases in single UVB-exposed human skin cells. PLoS One 12 (5) , e0176699 (2017).
7) Beims, R., Hu, Y., Shui, H., Xu, C.: Hydrothermal liquefaction of biomass to fuels and value-added chemicals: Products applications and challenges to develop large-scale operations. Biomass Bioenergy 135, 105510 (2020).
13) Takada, M., Rabemanolontsoa, H., Minami, E., Saka, S.: Characterization of lignin-derived products from various lignocellulosics as treated by semi-flow hot-compressed water. J. Wood Sci. 64, 802-809 (2018).
10) Yu, Y., Lou, X., Wu, H.: Some recent advances in hydrolysis of biomass in hot-compressed water and its comparisons with other hydrolysis methods. Energy Fuels 22 (1) , 46-60 (2008).
3) Kim, S., Lee, S., Hong, C., Gwak, K., Park, M., Smith, D., Choi, I.: Whitening and antioxidant activities of bornyl acetate and nezukol fractionated from Cryptomeria japonica essential oil. Int. J. Cosmet. Sci. 35 (5) , 484-490 (2013).
1) Horiba, H., Nakagawa, T., Zhu, Q., Ashour, A., Watanabe, A., Shimizu, K.: Biological activities of extracts from different parts of Cryptomeria japonica. Nat. Prod. Commun. 11 (9) , 1337-1342 (2016).
23) Figueroa, D., Asaduzzaman, M., Young, F.: Real time monitoring and quantification of reactive oxygen species in breast cancer cell line MCF-7 by 2',7'-dichlorofluorescin diacetate (DCFDA) assay. J. Pharmacol. Toxicol. Methods 94 (1) , 26-33 (2018).
21) Kwon, K., Alam, M., Park, J., Kim, T., Lee, S.: Attenuation of UVB-induced photo-aging by polyphenolic-rich spatholobus suberectus stem extract via modulation of MAPK/AP-1/MMPs signaling in human keratinocytes. Nutrients 11 (6) , 1341 (2019).
18) 沖 智之：DPPHラジカル消去活性評価法. “食品機能性評価マニュアル集第II集”, 日本食品科学工学会, pp.71-78, http://fmric.or.jp/ffd/ffmanual/100753_oki.pdf 2021年4月2日参照.
29) Kim, S., Kang, O., Joung, D., Mun, S., Seo, Y., Cha, M., Ryu, S., Shin, D., Kwon, D.: Anti-inflammatory effects of tectroside on UVB-induced HaCaT cells. Int. J. Mol. Med. 31 (6) , 1471-1476 (2013).
2) Yoon, W., Kim, S., Oh, T., Lee, N., Hyun, C.: Cryptomeria japonica essential oil inhibits the growth of drug-resistant skin pathogens and LPS-induced nitric oxide and pro-inflammatory cytokine production. Pol. J. Microbiol. 58 (1) , 61-68 (2009).
16) Brandner, D., Kruger, J., Thornburg, N., Facas, G., Kenny, J., Dreiling, R., Morais, A., Renders, T., Cleveland, N., Happs, R., Katahira, R., Vinzant, T., Wilcox, D., Román-Leshkov, Y., Beckham, G.: Flow-through solvolysis enables production of native-like lignin from biomass. Green Chemistry 23, 5437-5441 (2021).
9) Mishra, R., Kumar, V., Kumar, P., Mohanty, K.: Hydrothermal liquefaction of biomass for bio-crude production: A review on feedstocks, chemical compositions, operating parameters, reaction kinetics, techno-economic study, and life cycle assessment. Fuel 316, 123377 (2022).
25) Ishikura, N., Teramoto, S.: Procyanidins and catechin from callus and cell suspension cultures of Cryptomeria japonica. Agric. Biol. Chem. 47 (2) , 421-423 (1983).
References_xml – reference: 14) Kumagai, S., Hayashi, N., Sasaki, T., Nakada, M., Shibata, M.: Fractionation and saccharification of cellulose and hemicellulose in rice hull by hot-compressed-water treatment with two-step heating. J. Japan Inst. Ener. 83, 776-781 (2004).
– reference: 5) Cheng, S., Chua, M., Chang, E., Huang, C., Chen, W., Chang, S.: Variations in insecticidal activity and chemical compositions of leaf essential oils from Cryptomeria japonica at different ages. Bioresour. Technol. 100 (1) , 465-470 (2009).
– reference: 22) Costa, A., Facchini, G., Pinheiro, A., Silva, M., Bonner, M., Arbiser, J., Eberlin, S.: Honokiol protects skin cells against inflammation, collagenolysis, apoptosis, and senescence caused by cigarette smoke damage. Int. J. Dermatol. 56 (7) , 754-761 (2017).
– reference: 16) Brandner, D., Kruger, J., Thornburg, N., Facas, G., Kenny, J., Dreiling, R., Morais, A., Renders, T., Cleveland, N., Happs, R., Katahira, R., Vinzant, T., Wilcox, D., Román-Leshkov, Y., Beckham, G.: Flow-through solvolysis enables production of native-like lignin from biomass. Green Chemistry 23, 5437-5441 (2021).
– reference: 30) Wang, S., Lai, W., Chu, F., Lin, C., Shen, S., Chang, S.: Essential oil from the leaves of Cryptomeria japonica acts as a silverfish (Lepisma saccharina) repellent and insecticide. J. Wood Sci. 52, 522-526 (2006).
– reference: 24) Teramoto, S., Ishikura, N.: The formation of catechin and procyanidins in cell suspension cultures of Cryptomeria japonica. Shokubutsu-gaku-zasshi 98, 171-179 (1985).
– reference: 27) 上田裕人, 菅原進太郎, 松田 靖, 村田達郎, 黒田泰弘, 星 良和, 椛田聖孝, 井越敬司, 小野政輝, 安田 伸：2段階熱処理による熊本県産ヤーコン葉の抗酸化活性とフェノール成分に及ぼす影響. 東海大学先進生命科学研究所紀要 2, 29-34 (2018).
– reference: 4) Matsunaga, T., Hasegawa, C., Kawasuji, T., Suzuki, H., Saito, H., Sagioka, T., Takahashi, R., Tsukamoto, H., Morikawa, T., Akiyama, T.: Isolation of the antiulcer compound in essential oil from the leaves of Cryptomeria japonica. Biol. Pharm. Bull. 23 (5) , 595-598 (2000).
– reference: 10) Yu, Y., Lou, X., Wu, H.: Some recent advances in hydrolysis of biomass in hot-compressed water and its comparisons with other hydrolysis methods. Energy Fuels 22 (1) , 46-60 (2008).
– reference: 28) Gunaseelan, S., Balupillai, A., Govindasamy, K., Ramasamy, K., Muthusamy, G., Shanmugam, M., Thangaiyan, R., Robert, B., Nagarajan, R., Ponniresan, V., Rathinaraj, P.: Linalool prevents oxidative stress activated protein kinases in single UVB-exposed human skin cells. PLoS One 12 (5) , e0176699 (2017).
– reference: 6) Cha, J., Jeong, M., Jeong, S., Moon, S., Kil, B., Yun, S., Lee, K., Song, Y.: Chemical composition and antimicrobial activity of the essential oil of Cryptomeria japonica. Phytother. Res. 21 (3) , 295-299 (2007).
– reference: 7) Beims, R., Hu, Y., Shui, H., Xu, C.: Hydrothermal liquefaction of biomass to fuels and value-added chemicals: Products applications and challenges to develop large-scale operations. Biomass Bioenergy 135, 105510 (2020).
– reference: 25) Ishikura, N., Teramoto, S.: Procyanidins and catechin from callus and cell suspension cultures of Cryptomeria japonica. Agric. Biol. Chem. 47 (2) , 421-423 (1983).
– reference: 12) Yuliansyah, A., Kumagai, S., Hirajima, T., Sasaki, K.: Hydrothermal treatment of oil palm biomass in batch and semi-flow reactors. Energy Procedia 158, 675-680 (2019).
– reference: 15) Abdullah, R., Ueda, K., Saka, S.: Hydrothermal decomposition of various crystalline celluloses as treated by semi-flow hot-compressed water. J. Wood Sci. 60, 278-286 (2014).
– reference: 21) Kwon, K., Alam, M., Park, J., Kim, T., Lee, S.: Attenuation of UVB-induced photo-aging by polyphenolic-rich spatholobus suberectus stem extract via modulation of MAPK/AP-1/MMPs signaling in human keratinocytes. Nutrients 11 (6) , 1341 (2019).
– reference: 2) Yoon, W., Kim, S., Oh, T., Lee, N., Hyun, C.: Cryptomeria japonica essential oil inhibits the growth of drug-resistant skin pathogens and LPS-induced nitric oxide and pro-inflammatory cytokine production. Pol. J. Microbiol. 58 (1) , 61-68 (2009).
– reference: 26) 熊谷 聡, 林 信行, 坂木 剛, 中田正夫, 柴田昌男：加圧熱水2段階昇温による籾殻中セルロース, ヘミセルロースの成分分離と糖化. 日本エネルギー学会誌 83(10) , 776-781 (2004).
– reference: 3) Kim, S., Lee, S., Hong, C., Gwak, K., Park, M., Smith, D., Choi, I.: Whitening and antioxidant activities of bornyl acetate and nezukol fractionated from Cryptomeria japonica essential oil. Int. J. Cosmet. Sci. 35 (5) , 484-490 (2013).
– reference: 18) 沖 智之：DPPHラジカル消去活性評価法. “食品機能性評価マニュアル集第II集”, 日本食品科学工学会, pp.71-78, http://fmric.or.jp/ffd/ffmanual/100753_oki.pdf 2021年4月2日参照.
– reference: 9) Mishra, R., Kumar, V., Kumar, P., Mohanty, K.: Hydrothermal liquefaction of biomass for bio-crude production: A review on feedstocks, chemical compositions, operating parameters, reaction kinetics, techno-economic study, and life cycle assessment. Fuel 316, 123377 (2022).
– reference: 8) Scapini, T., Dos Santos, M., Bonatto, C., Wancura, J., Mulinari, J., Camargo, A., Klanovicz, N., Zabot, G., Tres, M., Fongaro, G., Treichel, H.: Hydrothermal pretreatment of lignocellulosic biomass for hemicellulose recovery. Bioresour. Technol. 342, 126033 (2021).
– reference: 11) Yu, Y., Wu, H.: Understanding the primary liquid products of cellulose hydrolysis in hot-compressed water at various reaction temperatures. Energy Fuels 24 (3) , 1963-1971 (2010).
– reference: 29) Kim, S., Kang, O., Joung, D., Mun, S., Seo, Y., Cha, M., Ryu, S., Shin, D., Kwon, D.: Anti-inflammatory effects of tectroside on UVB-induced HaCaT cells. Int. J. Mol. Med. 31 (6) , 1471-1476 (2013).
– reference: 20) Gao, X., Xu, D., Zhang, X., Zhao, H.: Protective effect of lemon peel polyphenols on oxidative stress-induced damage to human keratinocyte HaCaT cells through activation of the Nrf2/HO-1 signaling pathway. Front. Nutr. 7, 606776 (2021).
– reference: 1) Horiba, H., Nakagawa, T., Zhu, Q., Ashour, A., Watanabe, A., Shimizu, K.: Biological activities of extracts from different parts of Cryptomeria japonica. Nat. Prod. Commun. 11 (9) , 1337-1342 (2016).
– reference: 13) Takada, M., Rabemanolontsoa, H., Minami, E., Saka, S.: Characterization of lignin-derived products from various lignocellulosics as treated by semi-flow hot-compressed water. J. Wood Sci. 64, 802-809 (2018).
– reference: 17) 佐藤友紀, 上原健二, 工藤真二, 榊, 郁子, 林, 潤一郎, 進藤, 昌：シイタケ (Lentinula edodes) 廃菌床2段階温熱水パーコレーション抽出液の抗酸化活性. 木材学会誌 68(1) , 26-35(2022).
– reference: 19) 沖 智之：総ポリフェノールの定量法. “食品機能性評価マニュアル集第III集”, 日本食品 科学工学会, pp.1-7, http://fmric.or.jp/ffd/ffmanual/100753_oki.pdf 2021年4月2日参照.
– reference: 23) Figueroa, D., Asaduzzaman, M., Young, F.: Real time monitoring and quantification of reactive oxygen species in breast cancer cell line MCF-7 by 2',7'-dichlorofluorescin diacetate (DCFDA) assay. J. Pharmacol. Toxicol. Methods 94 (1) , 26-33 (2018).
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Snippet スギ（Cryptomeria japonica）の木材は，建材や家具，伝統工芸品などに広く使用される。しかし，スギの葉は，ほとんど使用されることなく，廃棄されてしまう。本研究では，水を溶媒とする2段階温熱水パーコレーションによる秋田スギ葉抽出物の生理活性をin...
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SourceType Publisher
StartPage 109
Title スギ（Cryptomeria japonica）葉2段階温熱水パーコレーション抽出物の抗酸化活性
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Volume 69
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ispartofPNX 木材学会誌, 2023/07/25, Vol.69(3), pp.109-116
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