日本国内におけるマダニ遺伝子データベースの構築:マダニミトコンドリア16S rDNAを用いた遺伝学的同定法確立の試み
マダニはライム病や日本紅斑熱などの起因病原体を伝播することから，人体刺咬例等のマダニ種同定の為に，迅速同定法開発が急務とされている．加えて，マダニの同定は形態学的な手法がGold standardであるが，虫体が欠損している場合などは形態同定が困難となる場合がある．このため，マダニ種同定の一助として，マダニミトコンドリア16S rRNA(mt-rrs)DNA配列の遺伝子データベースを構築し，これをもとにした系統学的な解析を行うとともに，その遺伝学的同定法の感度を調べた．国内に生息するマダニ47種中，39種について解析を行い，この内36種(92.3%)がDNA配列により区別できたことから，今後，...
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| Published in | 衛生動物 Vol. 65; no. 1; pp. 13 - 21 |
|---|---|
| Main Authors | , , , , , , , , , , , , , , , , |
| Format | Journal Article |
| Language | English Japanese |
| Published |
日本衛生動物学会
2014
|
| Online Access | Get full text |
| ISSN | 0424-7086 2185-5609 |
| DOI | 10.7601/mez.65.13 |
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| Abstract | マダニはライム病や日本紅斑熱などの起因病原体を伝播することから，人体刺咬例等のマダニ種同定の為に，迅速同定法開発が急務とされている．加えて，マダニの同定は形態学的な手法がGold standardであるが，虫体が欠損している場合などは形態同定が困難となる場合がある．このため，マダニ種同定の一助として，マダニミトコンドリア16S rRNA(mt-rrs)DNA配列の遺伝子データベースを構築し，これをもとにした系統学的な解析を行うとともに，その遺伝学的同定法の感度を調べた．国内に生息するマダニ47種中，39種について解析を行い，この内36種(92.3%)がDNA配列により区別できたことから，今後，マダニ形態同定が困難な場合でもその迅速同定が可能になると思われる．本研究で決定した全ての配列はGenBankに登録を行い，公共利用を可能としている． |
|---|---|
| AbstractList | マダニはライム病や日本紅斑熱などの起因病原体を伝播することから，人体刺咬例等のマダニ種同定の為に，迅速同定法開発が急務とされている．加えて，マダニの同定は形態学的な手法がGold standardであるが，虫体が欠損している場合などは形態同定が困難となる場合がある．このため，マダニ種同定の一助として，マダニミトコンドリア16S rRNA(mt-rrs)DNA配列の遺伝子データベースを構築し，これをもとにした系統学的な解析を行うとともに，その遺伝学的同定法の感度を調べた．国内に生息するマダニ47種中，39種について解析を行い，この内36種(92.3%)がDNA配列により区別できたことから，今後，マダニ形態同定が困難な場合でもその迅速同定が可能になると思われる．本研究で決定した全ての配列はGenBankに登録を行い，公共利用を可能としている． |
| Author | 御供田, 睦代 本田, 俊郎 川端, 寛樹 矢野, 泰弘 藤田, 博己 高田, 伸弘 山内, 健生 及川, 陽三郎 石畝, 史 安藤, 匡子 安藤, 秀二 高橋, 守 角坂, 照貴 鶴見, みや古 佐藤, 梢 角田, 隆 高野, 愛 |
| Author_xml | – sequence: 1 fullname: 本田, 俊郎 – sequence: 1 fullname: 高橋, 守 – sequence: 1 fullname: 高田, 伸弘 – sequence: 1 fullname: 安藤, 秀二 – sequence: 1 fullname: 角田, 隆 – sequence: 1 fullname: 及川, 陽三郎 – sequence: 1 fullname: 安藤, 匡子 – sequence: 1 fullname: 藤田, 博己 – sequence: 1 fullname: 高野, 愛 – sequence: 1 fullname: 石畝, 史 – sequence: 1 fullname: 佐藤, 梢 – sequence: 1 fullname: 矢野, 泰弘 – sequence: 1 fullname: 山内, 健生 – sequence: 1 fullname: 鶴見, みや古 – sequence: 1 fullname: 角坂, 照貴 – sequence: 1 fullname: 川端, 寛樹 – sequence: 1 fullname: 御供田, 睦代 |
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| References | Black, W. C. 4th, Klompen, J. S. and Keirans, J. E. 1997. Phylogenetic relationships among tick subfamilies (Ixodida: Ixodidae: Argasidae) based on the 18S nuclear rDNA gene. Mol. Phylogenet. Evol., 7: 129-144. Murrell, A., Campbell, N. J. and Barker, S. C. 2001. A total-evidence phylogeny of ticks provides insights into the evolution of life cycles and biogeography. Mol. Phylogenet. Evol., 21: 244-258. Okino, T., Ushirogawa, H., Matoba, K. and Hatsushika, R. 2010. Bibliographical Studies on Human Cases of Hard Tick (Acarina: Ixodidae) Bites in Japan (7) Cases of Unidentified Tick Infestation. Kawasaki Medical Journal, 36: 127-141, In Japanese with English abstract. Mangold, A. J., Bargues, M. D. and Mas-Coma, S. 1998. 18S rRNA gene sequences and phylogenetic relationships of European hard-tick species (Acari: Ixodidae). Parasitol. Res., 84: 31-37. Tamura, K., Peterson, D., Peterson, N., Stecher, G., Nei, M. and Kumar, S. 2011. MEGA5: Molecular Evolutionary Genetics Analysis using Maximum Likelihood, Evolutionary Distance, and Maximum Parsimony Methods. Mol. Biol. Evol., 28: 2731-2739. Ratnasingham, S. and Hebert, P. D. N. 2007. BOLD: The Barcode of Life Data System (www.barcodinglife.org). Mol. Ecol. Notes, 7: 355-364. Fukunaga, M., Yabuki, M., Hamase, A., Oliver, J. H. Jr. and Nakao, M. 2000. Molecular phylogenetic analysis of ixodid ticks based on the ribosomal DNA spacer, internal transcribed spacer 2, sequences. J. Parasitol., 86: 38-43. Ushijima, Y., Oliver, J. H. Jr., Keirans, J. E., Tsurumi, M., Kawabata, H., Watanabe, H. and Fukunaga, M. 2003. Mitochondrial sequence variation in Carios capensis (Neumann), a parasite of seabirds, collected on Torishima Island in Japan. J. Parasitol., 89: 196-198. Kawabata, H., Takano, A., Ohnishi, M., Ando, S., Ogasawara, Y., Fujita, H., Kadosaka, T., Wada, Y., Baba, S. and Seishima, M. 2011. Investigation of tick bite cases that visited medical facilities in Japan, from 2005 to June 2011. IASR, 32: 226-228, In Japanese. Black, W. C. 4th and Piesman, J. 1994. Phylogeny of hard- and soft-tick taxa (Acari: Ixodida) based on mitochondrial 16S rDNA sequences. Proc. Natl. Acad. Sci. U.S.A., 91: 10034-10038. Saitou, N. and Nei, M. 1987. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol. Biol. Evol., 4: 406-425. Fujita, H. and Takada, N. 2007. Identification of immature ticks in Japan. In: Acari and Emerging/Reemerging Infectious Diseases (ed. Yanagihara, Y.), pp. 53-68, Zenkoku Noson Kyoiku Kyokai Publishing, Tokyo (In Japanese). Guglielmone, A. A., Robbins, R. G., Apanaskevich, D. A., Petney, T. N., Estrada-Peña, A., Horak, I. G., Shao, R. and Barker, S. C. 2010. The Argasidae, Ixodidae and Nuttalliellidae (Acari: Ixodida) of the world: a list of valid species names. Zootaxa, 2528: 1-28. Jizhou, L., Wu, S., Zhang, Y., Zhang, T., Feng, C., Jia, G. and Lih, Z. 2013. Development of a barcode system for the Ixodida (Acari: Ixodida). Mitochondrial DNA, [Internet] Early online in Journal home [accessed Sep 13, 2013]. Available from: http://informahealthcare.com/doi/abs/10.3109/19401736.2013.792052. Fukunaga, M. 2007. Molecular phylogeny of ticks. In: Acari and Emerging / Reemerging Infectious Diseases (ed. Yanagihara, Y.), pp. 69-75, Zenkoku Noson Kyoiku Kyokai Publishing, Tokyo (In Japanese). Kimura, M. 1980. A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J. Mol. Evol., 16: 111-120. Bowman, A. S. and Nuttall, P. A. 2008. Ticks: biology, disease and control. 506 pp., Cambridge University Press, Cambridge. Beati, L. and Keirans, J. E. 2001. Analysis of the systematic relationships among ticks of the genera Rhipicephalus and Boophilus (Acari: Ixodidae) based on mitochondrial 12S ribosomal DNA gene sequences and morphological characters. J. Parasitol., 87: 32-48. |
| References_xml | – reference: Bowman, A. S. and Nuttall, P. A. 2008. Ticks: biology, disease and control. 506 pp., Cambridge University Press, Cambridge. – reference: Mangold, A. J., Bargues, M. D. and Mas-Coma, S. 1998. 18S rRNA gene sequences and phylogenetic relationships of European hard-tick species (Acari: Ixodidae). Parasitol. Res., 84: 31-37. – reference: Jizhou, L., Wu, S., Zhang, Y., Zhang, T., Feng, C., Jia, G. and Lih, Z. 2013. Development of a barcode system for the Ixodida (Acari: Ixodida). Mitochondrial DNA, [Internet] Early online in Journal home [accessed Sep 13, 2013]. Available from: http://informahealthcare.com/doi/abs/10.3109/19401736.2013.792052. – reference: Guglielmone, A. A., Robbins, R. G., Apanaskevich, D. A., Petney, T. N., Estrada-Peña, A., Horak, I. G., Shao, R. and Barker, S. C. 2010. The Argasidae, Ixodidae and Nuttalliellidae (Acari: Ixodida) of the world: a list of valid species names. Zootaxa, 2528: 1-28. – reference: Fukunaga, M. 2007. Molecular phylogeny of ticks. In: Acari and Emerging / Reemerging Infectious Diseases (ed. Yanagihara, Y.), pp. 69-75, Zenkoku Noson Kyoiku Kyokai Publishing, Tokyo (In Japanese). – reference: Kawabata, H., Takano, A., Ohnishi, M., Ando, S., Ogasawara, Y., Fujita, H., Kadosaka, T., Wada, Y., Baba, S. and Seishima, M. 2011. Investigation of tick bite cases that visited medical facilities in Japan, from 2005 to June 2011. IASR, 32: 226-228, In Japanese. – reference: Ratnasingham, S. and Hebert, P. D. N. 2007. BOLD: The Barcode of Life Data System (www.barcodinglife.org). Mol. Ecol. Notes, 7: 355-364. – reference: Saitou, N. and Nei, M. 1987. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol. Biol. Evol., 4: 406-425. – reference: Fujita, H. and Takada, N. 2007. Identification of immature ticks in Japan. In: Acari and Emerging/Reemerging Infectious Diseases (ed. Yanagihara, Y.), pp. 53-68, Zenkoku Noson Kyoiku Kyokai Publishing, Tokyo (In Japanese). – reference: Black, W. C. 4th and Piesman, J. 1994. Phylogeny of hard- and soft-tick taxa (Acari: Ixodida) based on mitochondrial 16S rDNA sequences. Proc. Natl. Acad. Sci. U.S.A., 91: 10034-10038. – reference: Okino, T., Ushirogawa, H., Matoba, K. and Hatsushika, R. 2010. Bibliographical Studies on Human Cases of Hard Tick (Acarina: Ixodidae) Bites in Japan (7) Cases of Unidentified Tick Infestation. Kawasaki Medical Journal, 36: 127-141, In Japanese with English abstract. – reference: Beati, L. and Keirans, J. E. 2001. Analysis of the systematic relationships among ticks of the genera Rhipicephalus and Boophilus (Acari: Ixodidae) based on mitochondrial 12S ribosomal DNA gene sequences and morphological characters. J. Parasitol., 87: 32-48. – reference: Ushijima, Y., Oliver, J. H. Jr., Keirans, J. E., Tsurumi, M., Kawabata, H., Watanabe, H. and Fukunaga, M. 2003. Mitochondrial sequence variation in Carios capensis (Neumann), a parasite of seabirds, collected on Torishima Island in Japan. J. Parasitol., 89: 196-198. – reference: Kimura, M. 1980. A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J. Mol. Evol., 16: 111-120. – reference: Black, W. C. 4th, Klompen, J. S. and Keirans, J. E. 1997. Phylogenetic relationships among tick subfamilies (Ixodida: Ixodidae: Argasidae) based on the 18S nuclear rDNA gene. Mol. Phylogenet. Evol., 7: 129-144. – reference: Murrell, A., Campbell, N. J. and Barker, S. C. 2001. A total-evidence phylogeny of ticks provides insights into the evolution of life cycles and biogeography. Mol. Phylogenet. Evol., 21: 244-258. – reference: Fukunaga, M., Yabuki, M., Hamase, A., Oliver, J. H. Jr. and Nakao, M. 2000. Molecular phylogenetic analysis of ixodid ticks based on the ribosomal DNA spacer, internal transcribed spacer 2, sequences. J. Parasitol., 86: 38-43. – reference: Tamura, K., Peterson, D., Peterson, N., Stecher, G., Nei, M. and Kumar, S. 2011. MEGA5: Molecular Evolutionary Genetics Analysis using Maximum Likelihood, Evolutionary Distance, and Maximum Parsimony Methods. Mol. Biol. Evol., 28: 2731-2739. |
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| Snippet | マダニはライム病や日本紅斑熱などの起因病原体を伝播することから，人体刺咬例等のマダニ種同定の為に，迅速同定法開発が急務とされている．加えて，マダニの同定は形態学的な手法がGold... |
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| Title | 日本国内におけるマダニ遺伝子データベースの構築:マダニミトコンドリア16S rDNAを用いた遺伝学的同定法確立の試み |
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