Detection of the magnetic fields of iPS cell-derived cardiomyocytes using simulated waveforms

• Published in: Transactions of Japanese Society for Medical and Biological Engineering Vol. Annual59; no. Proc; pp. 788 - 790
• Main Authors: Yamaguchi, Takeshi, Adachi, Yoshiaki, Tanida, Takashi, Oka, Yoshinobu, Yoshida, Takashi, Takahashi, Kenji, Tanaka, Masaki
• Format: Journal Article
• Language: Japanese
• Published: Japanese Society for Medical and Biological Engineering 2021
• ISSN: 1347-443X; 1881-4379
• DOI: 10.11239/jsmbe.Annual59.788

Currently, iPS cell-derived cardiomyocytes are important as cell materials, and a high-speed, automated quality evaluation method is required for their practical application. Focusing on the fact that magnetic field measurement is non-invasive, non-contact, and non-destructive, we investigated a method for evaluating cells by measuring the magnetic field with SQUID magnetometer. In this study, we developed an effective method for detecting small biological signals of cultured cells from measurement data. First, mathematical models that reproduce the action potentials of mouse iPS cell-derived cardiomyocytes were developed. Next, we simulated the magnetic field generated from the cultured cell population. Furthermore, deep learning was performed using the simulated magnetic field waveforms, and measurement data from cardiomyocytes were analyzed by the trained network. As a result, we succeeded in detecting magnetic signals that periodically correspond to the beats observed with an optical microscope and the field potentials, thus demonstrating the effectiveness of this method.