The Influence of the Acid Hot Spring Bathing at Tamagawa Spring on the Nucleic Acid of the Liver Tissues of Rabbits

• Published in: The Journal of Japanese Balneo-Climatological Association Vol. 25; no. 3; pp. 227 - 232
• Main Authors: SUGIE, Chunosuke, KATO, Hiromichi, BAN, Kiosyhi, RA, Kikan, IWASAKI, Zensuke, SO, Teiki
• Format: Journal Article
• Language: Japanese
• Published: THE JAPANESE SOCIETY OF BALNEOLOGY, CLIMATOLOGY AND PHYSICAL MEDICINE 1961; 一般社団法人 日本温泉気候物理医学会
• ISSN: 0369-4240; 1884-3689
• DOI: 10.11390/onki1935.25.227

The authors made a research on the effect of the acid hot spring bathing at Tamagawa Spring (an acid vitriol sulphur spring) upon the nucleic acid of the liver tissues of rabbits. The backs of rabbits were shaved, and some of them received a bathing for 10 minutes at 42-43°C and others serial bathing for 14 days (10 minutes once a day at 42-43°C). In the cases of single bathing, the examinations were done in 30 minutes, 24 hours, 3 clays and in 7 days after bathing, while in the cases of serial bathing, it was done on the 3rd, 7th, 10th, and 14th days of the bathing and in 3 days and 7 days after the end of the serial bathing. For dyeing of RNA of liver tissues, we used a pyronin-methyl-green staining, ribonuclease digestion and treatment with perchloric acid. For estimating the contents of RNA, the authors newly devised a standard in which the cells were classified under 11 types according to the size and the number of nucleolus, and the RNA contents were estimated in 200 liver cells of each rabbit. Results of the experiment; 1) The RNA contents of the liver tissue of the normal control group, being separetely observed at the central, the intermediary and the perilobular areas of the lobules, showed the following values in each type of those three areas. Type 0 was 1.3-1.6%, type 1: 0.8-1.5%, type 2: 7.7-7.8%, type 3: 12.9-14.5%, type 4: 2-2.7%, type 5: 0.5-0.8%. type 6: 13.8-15.7%, type 7: 17-20.5%, type 8: 30.7-34%, type 9: 5.5-7.7%, and type 10: 0-0.3%, respectively. 2) In the single bathing group, the cells of types 6-10 which contained a lot of RNA increased remarkably in 24 hours after bathing and increased slightly in 7 days after bathing. In 30 minutes and in 3 days after bathing, howerver, the RNA contents showed the normal values. 3) In the serial bathing group, on the 3rd day of this bathing, the cells of types 6-10 tended to increase slightly and decreased on 7th and 10th days. Then, in 3 days and 7 days after the end of the serial bathing, the cells of types 6-10 proved to increase remarkably.