Effective NADH-Dependent Oxidation of 7β-Hydroxy-Δ8-tetrahydrocannabinol to the Corresponding Ketone by Japanese Monkey Hepatic Microsomes

• Published in: Biological and Pharmaceutical Bulletin Vol. 28; no. 4; pp. 646 - 651
• Main Authors: Matsunaga, Tamihide, Higuchi, Shinsuke, Watanabe, Kazuhito, Kageyama, Takashi, Ohmori, Shigeru, Yamamoto, Ikuo
• Format: Journal Article
• Language: English
• Published: The Pharmaceutical Society of Japan 2005
• Subjects: 7-hydroxy-Δ8-tetrahydrocannabinol; CYP3A8; microsomal alcohol oxygenase; monkey; NADH; P450

The NADH-dependent activity by hepatic microsomes of Japanese monkeys for 7-oxo-Δ8-tetrahydrocannabinol (7-oxo-Δ8-THC) formation from 7β-hydroxy-Δ8-THC exhibited about 70% of the NADPH-dependent activity (100%) at the substrate concentration of 72.7 μM, although NADPH was an obligatory cofactor for...