Change for Aging of Bone and Liver Alkaline Phosphatase Isoenzymes in Serum of Healthy Subjects by Lectin Affinity Electrophoresis
ほぼ健常な小児患者と健常者の約1100名の血清を試料として, アルカリホスファターゼ (ALP: EC3.1.3.1.) の骨型および肝型アイソエンザイムをレクチン親和電気泳動法により求めた。活性値の測定はp-nitrophenyl phosphate基質での初速度法によった。生後14日までの新生児の骨型ALPは成人25歳の約3倍の活性値であった。一方肝型ALPは成人25歳とは大きな差は認められなかった。骨型ALPは生後8ヵ月前後までは上昇するが, その後減少し, 男児は 4歳, 女児は2歳で最低値になり, その後上昇して男児は13歳, 女児は 9歳で最高値に達した。以後男女共減少するが, 女...
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| Published in | Japanese Journal of Clinical Chemistry Vol. 19; no. 4; pp. 374 - 380 |
|---|---|
| Main Authors | , , , , |
| Format | Journal Article |
| Language | Japanese |
| Published |
Japan Society of Clinical Chemistry
1990
一般社団法人 日本臨床化学会 |
| Subjects | |
| Online Access | Get full text |
| ISSN | 0370-5633 2187-4077 |
| DOI | 10.14921/jscc1971b.19.4_374 |
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| Abstract | ほぼ健常な小児患者と健常者の約1100名の血清を試料として, アルカリホスファターゼ (ALP: EC3.1.3.1.) の骨型および肝型アイソエンザイムをレクチン親和電気泳動法により求めた。活性値の測定はp-nitrophenyl phosphate基質での初速度法によった。生後14日までの新生児の骨型ALPは成人25歳の約3倍の活性値であった。一方肝型ALPは成人25歳とは大きな差は認められなかった。骨型ALPは生後8ヵ月前後までは上昇するが, その後減少し, 男児は 4歳, 女児は2歳で最低値になり, その後上昇して男児は13歳, 女児は 9歳で最高値に達した。以後男女共減少するが, 女性は50歳代から再上昇した。肝型ALPは, 男女共1歳未満は若干低く、以後少し上昇して, 13歳頃までは大きな変動はなかった。そして15歳前後に若干低下して, その後 80歳まで, ゆるやかに上昇した。 |
|---|---|
| AbstractList | ほぼ健常な小児患者と健常者の約1100名の血清を試料として, アルカリホスファターゼ (ALP: EC3.1.3.1.) の骨型および肝型アイソエンザイムをレクチン親和電気泳動法により求めた。活性値の測定はp-nitrophenyl phosphate基質での初速度法によった。生後14日までの新生児の骨型ALPは成人25歳の約3倍の活性値であった。一方肝型ALPは成人25歳とは大きな差は認められなかった。骨型ALPは生後8ヵ月前後までは上昇するが, その後減少し, 男児は 4歳, 女児は2歳で最低値になり, その後上昇して男児は13歳, 女児は 9歳で最高値に達した。以後男女共減少するが, 女性は50歳代から再上昇した。肝型ALPは, 男女共1歳未満は若干低く、以後少し上昇して, 13歳頃までは大きな変動はなかった。そして15歳前後に若干低下して, その後 80歳まで, ゆるやかに上昇した。 |
| Author | Kuwana, Tomomi Wada, Reiko Hirokawa, Keiko Yakata, Minoru Sugita, Osamu |
| Author_FL | 屋形 稔 和田 玲子 桑名 友美 杉田 収 広川 恵子 |
| Author_FL_xml | – sequence: 1 fullname: 杉田 収 – sequence: 2 fullname: 和田 玲子 – sequence: 3 fullname: 桑名 友美 – sequence: 4 fullname: 広川 恵子 – sequence: 5 fullname: 屋形 稔 |
| Author_xml | – sequence: 1 fullname: Wada, Reiko organization: Clinical Laboratory, Niigata University Hospital – sequence: 1 fullname: Sugita, Osamu organization: Clinical Laboratory, Niigata University Hospital – sequence: 1 fullname: Hirokawa, Keiko organization: Department of Pediatric Surgery, Niigata University Hospital – sequence: 1 fullname: Yakata, Minoru organization: Youkouen, Higashi Niigata Hospital – sequence: 1 fullname: Kuwana, Tomomi organization: Clinical Laboratory, Niigata University Hospital |
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| DocumentTitle_FL | レクチン親和電気泳動法による健常者の骨型・肝型アルカリホスファターゼアイソエンザイムの加齢変化 |
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| References | 7) The committee on enzymes of the Scandinavian Society for Clinical Chemistry and Clinical Physiology: Recommended methods for the determination of four enzymes in blood, Scand J Clin Lab Invest, 33: 291-306, 1974. 13) 中尾喜久, 高久史麿, 和田攻: 骨多孔症 (骨粗霧症), pp.641-643, 丸善, 東京, 1974. 12) Shephard MDS, Peake MJ and Walmsley RN: Quantitative method for determining serum alkaline phosphatase isoenzyme activity, II. Development and clinical application of method for measuring four serum alkaline phosphatase isoenzymes, J Clin Pathol, 39: 1031-1038, 1986. 15) Riggs BL and Melton LJ III: Involutional osteoporosis, N Engl J Med, 314: 1676-1686, 1986. 1) Moss DW and Whitby LG: A simplified heatinactivation method for investigating alkaline phosphatase isoenzymes in serum, Clin Chim Acta, 61: 63-71, 1975. 17) Abdalla HI, Hart DM, Lindsay R, Leggate I and Hooke A: Prevention of bone mineral loss in postmenopausal women by norethisterone, Obstet Gynecol, 66: 789-792, 1985. 4) Kuwana T, Sugita O and Yakata M: Reference limits of bone and liver alkaline phosphatase isoenzymes in the serum of healthy subjects according to age and sex as determined by wheat germ lectin affinity electrophoresis, Clin Chim Acta, 173: 273-280, 1988. 5) 杉田収: 骨型ALPアイソザイムの解釈, 検査と技術, 18: 249-252, 1990. 6) 日本臨床化学会分析部会関東支部小児臨床化学委員会: 小児の基準範囲設定法勧告法試案, 臨床化学 (補冊), 16: 312-321, 1987. 3) 桑名友美, 杉田収, 屋形稔: レクチン親和電気泳動法による血清アルカリホスファターゼ (ALP) アイソザイムの分画と意義, 臨床病理, 35: 1264-1268, 1987. 11) Stepan JJ, Tesarova A, Havranek T, Jodl J, Formankova J and Pacovsky V: Age and sex dependency of the biochemical indices of bone remodelling, Clin Chim Acta, 151: 273-283, 1985. 18) Deeny M, Farish E, Tillman J, Dagen M, Hart DM and Fletcher CD: Changes in the bone and liver isoenzymes of alkaline phosphatase in postmenopausal woman being treated with norethisterone, Clin Chim Acta, 171: 103-108, 1988. 16) Riggs 3L and Melton LJ III: Evidence for two distinct syndromes of involutional osteoporosis, Am J Med, 75: 899-901, 1983. 9) Hitz J, Daigle G, Petitclerc C, Schiele F and Siest G: Automated quantification of bone and liver alkaline phosphatase isoenzymes of human serum, Clin Chim Acta, 107: 203-210, 1980. 8) Whitaker KB, Whitby LG and Moss DW: Activities of bone and liver alkaline phosphatases in serum in health and disease, Clin Chim Acta, 80: 209-220, 1977. 14) Hui SL, Slemenda CW and Johnston CC: Age and bone mass as predictors of fracture in a prospective study, J Clin Invest, 81: 1804-1809, 1988. 2) Rosalki SB and Foo AY: Two new methods for separating and quantifying bone and liver alkaline phosphatase isoenzymes in plasma, Clin Chem, 30: 1182-1186, 1984. 10) Schiele F, Henny J, Hitz J, Petitclerc C, Gueguen R and Siest G: Total bone and liver alkaline phosphatases in plasma: Biological variations and reference limits, Clin Chem, 29: 634-641, 1983. |
| References_xml | – reference: 5) 杉田収: 骨型ALPアイソザイムの解釈, 検査と技術, 18: 249-252, 1990. – reference: 13) 中尾喜久, 高久史麿, 和田攻: 骨多孔症 (骨粗霧症), pp.641-643, 丸善, 東京, 1974. – reference: 10) Schiele F, Henny J, Hitz J, Petitclerc C, Gueguen R and Siest G: Total bone and liver alkaline phosphatases in plasma: Biological variations and reference limits, Clin Chem, 29: 634-641, 1983. – reference: 6) 日本臨床化学会分析部会関東支部小児臨床化学委員会: 小児の基準範囲設定法勧告法試案, 臨床化学 (補冊), 16: 312-321, 1987. – reference: 11) Stepan JJ, Tesarova A, Havranek T, Jodl J, Formankova J and Pacovsky V: Age and sex dependency of the biochemical indices of bone remodelling, Clin Chim Acta, 151: 273-283, 1985. – reference: 2) Rosalki SB and Foo AY: Two new methods for separating and quantifying bone and liver alkaline phosphatase isoenzymes in plasma, Clin Chem, 30: 1182-1186, 1984. – reference: 4) Kuwana T, Sugita O and Yakata M: Reference limits of bone and liver alkaline phosphatase isoenzymes in the serum of healthy subjects according to age and sex as determined by wheat germ lectin affinity electrophoresis, Clin Chim Acta, 173: 273-280, 1988. – reference: 14) Hui SL, Slemenda CW and Johnston CC: Age and bone mass as predictors of fracture in a prospective study, J Clin Invest, 81: 1804-1809, 1988. – reference: 16) Riggs 3L and Melton LJ III: Evidence for two distinct syndromes of involutional osteoporosis, Am J Med, 75: 899-901, 1983. – reference: 15) Riggs BL and Melton LJ III: Involutional osteoporosis, N Engl J Med, 314: 1676-1686, 1986. – reference: 9) Hitz J, Daigle G, Petitclerc C, Schiele F and Siest G: Automated quantification of bone and liver alkaline phosphatase isoenzymes of human serum, Clin Chim Acta, 107: 203-210, 1980. – reference: 17) Abdalla HI, Hart DM, Lindsay R, Leggate I and Hooke A: Prevention of bone mineral loss in postmenopausal women by norethisterone, Obstet Gynecol, 66: 789-792, 1985. – reference: 18) Deeny M, Farish E, Tillman J, Dagen M, Hart DM and Fletcher CD: Changes in the bone and liver isoenzymes of alkaline phosphatase in postmenopausal woman being treated with norethisterone, Clin Chim Acta, 171: 103-108, 1988. – reference: 1) Moss DW and Whitby LG: A simplified heatinactivation method for investigating alkaline phosphatase isoenzymes in serum, Clin Chim Acta, 61: 63-71, 1975. – reference: 12) Shephard MDS, Peake MJ and Walmsley RN: Quantitative method for determining serum alkaline phosphatase isoenzyme activity, II. Development and clinical application of method for measuring four serum alkaline phosphatase isoenzymes, J Clin Pathol, 39: 1031-1038, 1986. – reference: 7) The committee on enzymes of the Scandinavian Society for Clinical Chemistry and Clinical Physiology: Recommended methods for the determination of four enzymes in blood, Scand J Clin Lab Invest, 33: 291-306, 1974. – reference: 8) Whitaker KB, Whitby LG and Moss DW: Activities of bone and liver alkaline phosphatases in serum in health and disease, Clin Chim Acta, 80: 209-220, 1977. – reference: 3) 桑名友美, 杉田収, 屋形稔: レクチン親和電気泳動法による血清アルカリホスファターゼ (ALP) アイソザイムの分画と意義, 臨床病理, 35: 1264-1268, 1987. |
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| Title | Change for Aging of Bone and Liver Alkaline Phosphatase Isoenzymes in Serum of Healthy Subjects by Lectin Affinity Electrophoresis |
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