Isolation and Identification of Fucoidan from Okinawamozuku (Cladosiphon okamuranus TOKIDA)

A Fucoidan was isolated from Okinawamozuku (Cladosiphon okamuranus T0KmA) and its chemical and physicochemical properties were investigated . The total carbohydrates, uronic acid, ash, sulfuricacid, and moisture of the fucoidan was estimated to be 67.2, 13.5, 23.0, 11.9, and 3.2%, respectively. The...

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Published inJournal of Applied Glycoscience Vol. 43; no. 2; pp. 143 - 148
Main Authors UEHARA, Megumi, CHINEN, Isao, TAKO, Masakuni, HONGO, Fujiya, KAWASHIMA, Yoshitsugu
Format Journal Article
LanguageEnglish
Japanese
Published The Japanese Society of Applied Glycoscience 1996
日本応用糖質科学会
Online AccessGet full text
ISSN1340-3494
1884-4898
DOI10.11541/jag1994.43.143

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Abstract A Fucoidan was isolated from Okinawamozuku (Cladosiphon okamuranus T0KmA) and its chemical and physicochemical properties were investigated . The total carbohydrates, uronic acid, ash, sulfuricacid, and moisture of the fucoidan was estimated to be 67.2, 13.5, 23.0, 11.9, and 3.2%, respectively. The L-fucose and n-xylose residues were identified by paper and liquid chromatography from the acid hydrolysate of the fucoidan. The molecular weight of the fucoidan was estimated to be about 500, 000. The optical rotation of the fucoidan showed a value of -0.351° at a temperature of 60°C, then it decreased a little with decreasing temperature. The infrared spectrum of the fucoidan was essentially in agreement with that of a fucoidan (Sigma), which was used as a standard sample, in a wide range of wave numbers.
AbstractList A Fucoidan was isolated from Okinawamozuku (Cladosiphon okamuranus T0KmA) and its chemical and physicochemical properties were investigated . The total carbohydrates, uronic acid, ash, sulfuricacid, and moisture of the fucoidan was estimated to be 67.2, 13.5, 23.0, 11.9, and 3.2%, respectively. The L-fucose and n-xylose residues were identified by paper and liquid chromatography from the acid hydrolysate of the fucoidan. The molecular weight of the fucoidan was estimated to be about 500, 000. The optical rotation of the fucoidan showed a value of -0.351° at a temperature of 60°C, then it decreased a little with decreasing temperature. The infrared spectrum of the fucoidan was essentially in agreement with that of a fucoidan (Sigma), which was used as a standard sample, in a wide range of wave numbers.
Author TAKO, Masakuni
CHINEN, Isao
UEHARA, Megumi
HONGO, Fujiya
KAWASHIMA, Yoshitsugu
Author_FL 本郷 富士弥
川島 由次
田幸 正邦
知念 功
上原 めぐみ
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  fullname: KAWASHIMA, Yoshitsugu
  organization: Department of Environmental Sciences and Technology, College of Agriculture, University of the Ryukyus
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DocumentTitleAlternate オキナワモズクからフコイダンの分離・同定
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References_xml – reference: 19) 富士川龍郎,阿武尚彦}和田正太:農化,49,667-669(1975).
– reference: 20) T. A. ACCAFFREY, D. J. FALCONE, W. BORTH, C. F. BRAYTON and B. WEKSLER Biochem. Biophys. Res. Commum., 184, 773-781 (1992).
– reference: 22) M. 0. MCCLURE, D. WHITBY, C. PATIENCE, N. J. GOODERHAM, A. BRADSHAW, R. CHEINGSONG POPOV, J. N. WEBER, D. S. DAVIES, G. M. W. CooK, R. J. KEYNES and R. A. WEISS : ANTI VIRAL Chemother., 2, 149-156 (1991).
– reference: 2) M. TAKO and S. NAKAMURA : Carbohydr. Res., 155, 200-205 (1986).
– reference: 17) 前田昌徹西沢一俊:総合多糖類科学,下巻原田篤也,三崎旭編,講談社,東京,p.310(1976).
– reference: 4) M. TAKO, S. NAKAMURA and Y. KOHDA : Carbohydr. Res., 161, 247-255 (1987).
– reference: 1) R. L. WHISTLER : in Industrial Gums, 3rd ed., R. L, WHISTLER and J. N. BEMILLER, eds., Academic Press, New York, p.1 (1993).
– reference: 6) M. TAKO and S. NAKAMURA : Agric. Biol. Chem., 52, 1071-1072 (1988).
– reference: 11) 富士川龍郎,中島克子:農化,49,455-461(1975).
– reference: 5) M. TAKO and S. NAKAMURA : Carbohydr: Res., 180, 277-284 (1988).
– reference: 18) 森宏枝:海藻の生化学と利用,日本水産学会編,恒星社厚生閣,東京,p.33(1983).
– reference: 21) H. ITOH, H. NODA, H. AMANO, C. ZHUAUG, T. MIZUNO and H. ITOH : Anticancer Res., 13, 2045-2052 (1993).
– reference: 12) T. Fu11KAWA and M. WADA : Agric. Biol. Chem., 39, 1109-1114 (1975).
– reference: 16) 東京大学:東京大学農芸化学実験書,上巻,朝倉書店,東京,p.9(1966).
– reference: 13) 当間武:サンゴ礁域の増養殖,諸喜田茂充編,緑書房,東京,p.56(1988).
– reference: 10) M. ELLOUALI, C. BlossoN-VIDAL, P. DURAND and J. JOZEFoNvicz : Anticancer Res., 13, 2011-2029 (1993).
– reference: 9) T. NISHINO, Y. AIZU and T. NAOUMO : Agric. Biol. Chem., 55, 791-796 (1991).
– reference: 8) 田幸正邦:ニューフードインダストリー,34(10),65-82{1992}.
– reference: 15) J. T. GALAMBOS : Anal. Biochem., 19, 119-132 (1967).
– reference: 7) 田幸正邦:三ユーフードインダストリー,34(9),71-78(1992).
– reference: 3) M. TAKO and S. NAKAMURA : Agric. Biol. Chem., 50, 2817-2822 (1986).
– reference: 14) M. DUBOIS, K. A. GILLES, J. K. HAMILTON, P. A. BERBERS and F. SMITH : Anal. Chem., 28, 350-356 (1956).
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Title Isolation and Identification of Fucoidan from Okinawamozuku (Cladosiphon okamuranus TOKIDA)
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