A stoichiometrical and ultrastructural study on cell membrane damage by bile salt using human red blood cells

A study on cell membrane damage by bile salts was performed using human red blood cells (RBC). Taurochenodeoxycholate (TCDC), taurodeoxycholate (TDC), taurocholate (TC), and tauroursodeoxycholate (TUDC) were co-incubated with human RBC, respectively, followed by measurement of release of cell free h...

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Published inTando Vol. 5; no. 2; pp. 125 - 132
Main Authors TAO, Seishi, YAMASHITA, Gunji, YAMAMOTO, Masao, SAGAWA, Hiroshi, TAZUMA, SUSUMU, AIHARA, Naoki, KAJIYAMA, Goro, HATSUSHIKA, Sumie, HORIUCHI, Itaru, SASAKI, Harutoshi, MIZUNO, Shigeki
Format Journal Article
LanguageJapanese
Published Japan Biliary Association 1991
Subjects
胆汁酸
膜障害性
赤血球膜
透過型電子顕微鏡
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ISSN0914-0077
1883-6879
DOI10.11210/tando1987.5.2_125

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Abstract A study on cell membrane damage by bile salts was performed using human red blood cells (RBC). Taurochenodeoxycholate (TCDC), taurodeoxycholate (TDC), taurocholate (TC), and tauroursodeoxycholate (TUDC) were co-incubated with human RBC, respectively, followed by measurement of release of cell free hemoglobin and morphological determination by transmission electron microscopy (TEM). The hydrophobicity of each bile salt was indexed by the retention time of a reverse phase HPLC. Phosphatidylcholine (PC), cholesterol (CH), albumin, and mucin were tested for their ability to protect RBC' from damage by bile salts. Results were as follows; 1. The ranking of hemolytic effects of bile salt determined in stoichiometry and morphology was TDC> TCDC> TC> TUDC.2. A strong linear correlation between hemolytic effects of bile salt and their hydrophobicity was found (y=0.689x-0.054, r=0.991).3. PC and albumin reduced bile salt toxicity dose-dependently, but did neither CH nor mucin. Thus, we deduced that cell membranes including biliary systems are protected against hydrophobic bile salt toxicity by PC and certain proteins, i. e. albumin.
AbstractList A study on cell membrane damage by bile salts was performed using human red blood cells (RBC). Taurochenodeoxycholate (TCDC), taurodeoxycholate (TDC), taurocholate (TC), and tauroursodeoxycholate (TUDC) were co-incubated with human RBC, respectively, followed by measurement of release of cell free hemoglobin and morphological determination by transmission electron microscopy (TEM). The hydrophobicity of each bile salt was indexed by the retention time of a reverse phase HPLC. Phosphatidylcholine (PC), cholesterol (CH), albumin, and mucin were tested for their ability to protect RBC' from damage by bile salts. Results were as follows; 1. The ranking of hemolytic effects of bile salt determined in stoichiometry and morphology was TDC> TCDC> TC> TUDC.2. A strong linear correlation between hemolytic effects of bile salt and their hydrophobicity was found (y=0.689x-0.054, r=0.991).3. PC and albumin reduced bile salt toxicity dose-dependently, but did neither CH nor mucin. Thus, we deduced that cell membranes including biliary systems are protected against hydrophobic bile salt toxicity by PC and certain proteins, i. e. albumin.
A study on cell membrane damage by bile salts was performed using human red blood cells (RBC). Taurochenodeoxycholate (TCDC), taurodeoxycholate (TDC), taurocholate (TC), and tauroursodeoxycholate (TUDC) were co-incubated with human RBC, respectively, followed by measurement of release of cell free hemoglobin and morphological determination by transmission electron microscopy (TEM). The hydrophobicity of each bile salt was indexed by the retention time of a reverse phase HPLC.Phosphatidylcholine (PC), cholesterol (CH), albumin, and mucin were tested for their ability to protect RBC' from damage by bile salts. Results were as follows; 1. The ranking of hemolytic effects of bile salt determined in stoichiometry and morphology was TDC> TCDC> TC> TUDC.2. A strong linear correlation between hemolytic effects of bile salt and their hydrophobicity was found (y=0.689x-0.054, r=0.991).3. PC and albumin reduced bile salt toxicity dose-dependently, but did neither CH nor mucin. Thus, we deduced that cell membranes including biliary systems are protected against hydrophobic bile salt toxicity by PC and certain proteins, i. e. albumin. タウロデオキシコール酸(TDCA),タウロケノデオキシコール酸(TCDCA),タウロコール酸(TCA),タウロウルソデオキシコール酸(TUDCA)を用いて,各種胆汁酸の細胞膜障害性をヒト赤血球膜を用いて溶血を指標として検討した.その結果膜障害性の強さは,TDCA>TCDCA>TCA>TUDCAの順で濃度および時間依存性であった.一方,高送液体クロマトグラフィーで算定された各種胆汁酸の疎水性は膜障害性の強度と一致し,両者は正の相関を示した(r=0.99,p<0.01).さらに,その膜障害性は,ホスファチジルコリンおよび牛血清アルブミン添加によって阻止されたが,コレステロール,豚胃粘膜ムチン添加では阻止されなかった.透過型電子顕微鏡による赤血球膜表面の形態学検討では,胆汁酸による膜内粒子の溶出,微絨毛様の突起形成が認められた.以上より,胆汁酸はその界面活性作用によって,膜脂質二重層の構築を変化させることによって膜障害を惹起し,その強度は胆汁酸の疎水性によって規定されると考えられた.
Author SASAKI, Harutoshi
SAGAWA, Hiroshi
HATSUSHIKA, Sumie
AIHARA, Naoki
HORIUCHI, Itaru
YAMASHITA, Gunji
YAMAMOTO, Masao
TAZUMA, SUSUMU
MIZUNO, Shigeki
KAJIYAMA, Goro
TAO, Seishi
Author_FL 相原 直樹
峠 誠司
梶山 梧朗
水野 重樹
山本 正夫
佐々木 晴敏
山下 郡司
田妻 進
堀内 至
佐川 広
初鹿 寿美恵
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DocumentTitleAlternate 胆汁酸の膜障害性に関する研究(第1報)
ヒト赤血球膜を用いて
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References 4) Armstrong MJ, Carey MC: The hydrophobichydrophilic balance of bile salts. I n verse correlation between reversephase high performance liquid chromatographic mobilities and miceller cholesterol-solubilizing capacities. J Lipid Res, 23: 70-80, 1982
5)沼波良太: 諸種薬物の細胞膜に及ぼす影響. 昭医会誌45: 167-180, 1985
6)亀井勝彦: 胆汁酸の界面活性作用と膜障害について. 昭医会誌44: 213-220, 1984
7) Klrlaganis G, Paumgartner G: Determination of Bile Acids in Serum by Capillary Gas-Liquid chromatography. Clin Chim Act 92: 19-26, 1979
8) Ohkubo H, Okuda, K, Makino I: Role of Portal and Splenic Vein Shunt and Impaired Hepatic Extraction in the Elevated Serum Bile Acids in Liver Cirrhosis. Gastroenterology 86: 514, 1984
1)八尋克三: ケノデオキシコール酸投与による肝毒性発現機i構iに関する研究. 日消誌83: 2014-2021, 1986
9)向坂彰太郎, 案納弘子, 谷川久一: ケノデオキシコール酸とウルソデオキシコ一ル酸の培養ラット肝細胞に対する影響とpolyenephosphatidylchl1ine による肝細胞保護作用について. 肝臓25: 350-358, 1984
10) Tazuma 5, Sasaki H, Mizuno 5, et al: Effect of Ursodeoxycholic Acid Administration on Nucleation Time in Human Gallbaldder Bile. Gastroenterology 97: 173-178, 1989
2)成澤富雄: 胆汁酸による大腸発癌プロモーション機序. TOKYO TANABE QUARTERLY 臨時増刊: 18-29, 1989
11) Passing R, Schubert D: The Binding of Deoxycholic Acid Atcoid Bandz Protein from Hum an Erythrocyte Membranes and to Bovine Serum Albumin. Hoppe-Seyler's Z. Pysiol Chem 364: 219-226, 1983
3)木村恒夫: 胆汁酸の肝細胞障害性に関する研究. 日消誌77: 185-194, 1980
References_xml – reference: 1)八尋克三: ケノデオキシコール酸投与による肝毒性発現機i構iに関する研究. 日消誌83: 2014-2021, 1986
– reference: 11) Passing R, Schubert D: The Binding of Deoxycholic Acid Atcoid Bandz Protein from Hum an Erythrocyte Membranes and to Bovine Serum Albumin. Hoppe-Seyler's Z. Pysiol Chem 364: 219-226, 1983
– reference: 8) Ohkubo H, Okuda, K, Makino I: Role of Portal and Splenic Vein Shunt and Impaired Hepatic Extraction in the Elevated Serum Bile Acids in Liver Cirrhosis. Gastroenterology 86: 514, 1984
– reference: 7) Klrlaganis G, Paumgartner G: Determination of Bile Acids in Serum by Capillary Gas-Liquid chromatography. Clin Chim Act 92: 19-26, 1979
– reference: 5)沼波良太: 諸種薬物の細胞膜に及ぼす影響. 昭医会誌45: 167-180, 1985
– reference: 3)木村恒夫: 胆汁酸の肝細胞障害性に関する研究. 日消誌77: 185-194, 1980
– reference: 6)亀井勝彦: 胆汁酸の界面活性作用と膜障害について. 昭医会誌44: 213-220, 1984
– reference: 9)向坂彰太郎, 案納弘子, 谷川久一: ケノデオキシコール酸とウルソデオキシコ一ル酸の培養ラット肝細胞に対する影響とpolyenephosphatidylchl1ine による肝細胞保護作用について. 肝臓25: 350-358, 1984
– reference: 10) Tazuma 5, Sasaki H, Mizuno 5, et al: Effect of Ursodeoxycholic Acid Administration on Nucleation Time in Human Gallbaldder Bile. Gastroenterology 97: 173-178, 1989
– reference: 2)成澤富雄: 胆汁酸による大腸発癌プロモーション機序. TOKYO TANABE QUARTERLY 臨時増刊: 18-29, 1989
– reference: 4) Armstrong MJ, Carey MC: The hydrophobichydrophilic balance of bile salts. I n verse correlation between reversephase high performance liquid chromatographic mobilities and miceller cholesterol-solubilizing capacities. J Lipid Res, 23: 70-80, 1982
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Snippet A study on cell membrane damage by bile salts was performed using human red blood cells (RBC). Taurochenodeoxycholate (TCDC), taurodeoxycholate (TDC),...
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SubjectTerms 胆汁酸
膜障害性
赤血球膜
透過型電子顕微鏡
Title A stoichiometrical and ultrastructural study on cell membrane damage by bile salt using human red blood cells
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