Inositol synthesis gene is required for circadian rhythm of Drosophila melanogaster mating behavior

Accumulating evidence indicates that the molecular circadian clock underlies the mating behavior of Drosophila melanogaster. However, information about which gene affects circadian mating behavior is poorly understood in animals. The present study found that feeding Myo-inositol enhanced the close-p...

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Bibliographic Details
Published inbioRxiv
Main Authors Sakata, Kazuki, Kawasaki, Haruhisa, Ishida, Norio
Format Paper
LanguageEnglish
Published Cold Spring Harbor Cold Spring Harbor Laboratory Press 20.02.2020
Subjects
Animal reproduction
Butterflies & moths
Circadian rhythm
Circadian rhythms
Drosophila melanogaster
Foxes
Gene expression
Glucose
Insects
Mating behavior
RNA-mediated interference
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Summary:Accumulating evidence indicates that the molecular circadian clock underlies the mating behavior of Drosophila melanogaster. However, information about which gene affects circadian mating behavior is poorly understood in animals. The present study found that feeding Myo-inositol enhanced the close-proximity (CP) rhythm of D. melanogaster mating behavior and lengthened the period of the CP rhythm. Then, to understand a role for inositol synthesis to fly mating behavior, we established the Inos (Myo-inositol 1-phosphate synthase) gene knock down fly strains with RNAi. Interestingly, the CP behavior of this three-different driver knock down strains was arrhythmic, but the locomotor rhythm was rhythmic. The data of three-different Inos knock down strains suggests that Inos gene expression of upper LNd, l-LNV, 5ths-LNv in brain is necessary for proper CP rhythm generation in D. melanogaster. The data indicated that the Inos gene is involved in the role for the circadian rhythm of D. melanogaster mating behavior.
DOI:10.1101/2020.02.19.955583