α-fetoproteinに関する研究(V) 3'-Me-DABラット肝癌に対する抗AFP抗体投与の効果-その免疫組織化学的検討
3'-Me-DAB飼育ラットのある時期に抗AFP抗体を投与することにより,肝癌の発生および増殖を著明に抑制することを教室の佐藤が報告した.そこで著者は,その効果の機序を明らかにする目的で,抗AFP抗体投与ラットにおける肝のAFP産生細胞の変化,および投与された抗体の組織内局在について酵素抗体法を用いて検討した.その結果,3'-Me-DAB飼育の4週目に抗AFP特異抗体を投与した群において肝組織におけるAFP陽性細胞が著明に減少すること,しかも投与された抗体グロブリンが肝のある種の細胞に局在することを明らかにした.この成績より,投与された抗体と組織内のAFPとが細胞を場として...
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| Published in | 肝臓 Vol. 21; no. 12; pp. 1637 - 1646 |
|---|---|
| Main Author | |
| Format | Journal Article |
| Language | Japanese |
| Published |
一般社団法人 日本肝臓学会
25.12.1980
|
| Subjects | |
| Online Access | Get full text |
| ISSN | 0451-4203 1881-3593 |
| DOI | 10.2957/kanzo.21.1637 |
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| Abstract | 3'-Me-DAB飼育ラットのある時期に抗AFP抗体を投与することにより,肝癌の発生および増殖を著明に抑制することを教室の佐藤が報告した.そこで著者は,その効果の機序を明らかにする目的で,抗AFP抗体投与ラットにおける肝のAFP産生細胞の変化,および投与された抗体の組織内局在について酵素抗体法を用いて検討した.その結果,3'-Me-DAB飼育の4週目に抗AFP特異抗体を投与した群において肝組織におけるAFP陽性細胞が著明に減少すること,しかも投与された抗体グロブリンが肝のある種の細胞に局在することを明らかにした.この成績より,投与された抗体と組織内のAFPとが細胞を場として免疫反応を起こしている可能性が強く考えられ,それが抗体による腫瘍抑制効果の機序の一因であることが示唆された.索引用語:α-fetoprotein AFP 3'-Me-DAB抗AFP抗体 酵素抗体法 |
|---|---|
| AbstractList | 3'-Me-DAB飼育ラットのある時期に抗AFP抗体を投与することにより,肝癌の発生および増殖を著明に抑制することを教室の佐藤が報告した.そこで著者は,その効果の機序を明らかにする目的で,抗AFP抗体投与ラットにおける肝のAFP産生細胞の変化,および投与された抗体の組織内局在について酵素抗体法を用いて検討した.その結果,3'-Me-DAB飼育の4週目に抗AFP特異抗体を投与した群において肝組織におけるAFP陽性細胞が著明に減少すること,しかも投与された抗体グロブリンが肝のある種の細胞に局在することを明らかにした.この成績より,投与された抗体と組織内のAFPとが細胞を場として免疫反応を起こしている可能性が強く考えられ,それが抗体による腫瘍抑制効果の機序の一因であることが示唆された.索引用語:α-fetoprotein AFP 3'-Me-DAB抗AFP抗体 酵素抗体法 |
| Author | 鈴木, 裕二 |
| Author_xml | – sequence: 1 fullname: 鈴木, 裕二 organization: 日本大学医学部第3内科 |
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| Copyright | 社団法人 日本肝臓学会 |
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| References | 3) 上村直也:α-fetoproteinに関する研究(III),特にアゾ色素肝癌における二次反応初期のAFP産生細胞について.日大医誌,38 (3): 595, 1979. 4) Fujita, S.: The α-fetoprotein producing cells in the early stage of the experimental liver cancer. Ann. N.Y. Acad. Sci., 259: 217, 1975. 2) 石塚英夫:α;-fetoproteinに関する研究(II), 3'-Me-DAB肝癌発癌過程の早期におけるα-fetoprotein産生細胞について.日消誌,72 (1): 1224, 1975. 17) Dattwyler, J.R. & Tomasi, T.B.: Inhibition of sensitization of T-cells by alphafetoprotein. Int. J. Cancer, 16: 942, 1975. 11) Onoe, T.: Significance of α-fetoprotein appearence in the early stage of azo-dye carcinogenesis. Gann Monograph, 14: 233, 1973. 9) Nakane, P.K. & Kawaoi, A.: Peroxidaselabelled antibody. A new method of conjugation. J. Hitochem. Cytochem., 22 (12): 1084, 1974. 6) Kaneda, H.: Effect of anti-rat AFP horse serum on the development of hepatoma in the early 3'-Methyl-4-dimethylaminoazobenzene. Carcino-Embryonic Proteins, ed. Lehmann, F.G., Vol. 1, 1979, p.539. 7) Tsukada, T. & Mikuni, M.: Effect on antialpha-fetoprotein serum on some cultured tumor cells. Int. J. Cancer, 13: 187, 1974. 8) Watabe, H.: Early appearence of embryonic α-globulin in rat serum during carcinogenesis with 4-dimethylaminoazobenzene. Can. Res., 31: 1192, 1971. 21) Smith, J.A.: Alpha-fetoprotein; A possible factor necessary for normal development of the embryo. Lancet, 1: 851, 1972. 22) Slade, B.: Antibodies to α-fetoprotein cause fetal mortality in rabbits. Nature, 246: 493, 1973. 27) Alarcon-Segovia, D.A.: Antibody penetration into living cells 1. Intranuclear immunoglobulin in peripheral blood mononuclear cells in mixed connective tissue disease and systemic lupus erythematodes. Clin. Exp. Immunol., 36: 364, 1979. 13) Gold, P. & Freedman, S.O.: Demonstration of tumor specific antigen in human colonic carcinoma by immunological and absorption technique. J. Exp. Med., 121: 439, 1965. 20) Shinzo Nishi,: Species cross-reaction of α-fetoprotein and breakdown of the tolerance to α-fetoprotein by immunization with heterologous α-fetoprotein. Tumor, Res., 8: 17, 1973. 28) Mori, M. & Novicoff, A.B.: Induction of pinocytosis in rat hepatocytes by partial hepatectomy. J. Cell Biol., 72: 695, 1977. 25) Alarcon-Segovia, D.: Uracil-specific anti-RNA antibodies in scleroderma. Lancet, 1: 363, 1975. 16) Parmely, M.J.H.F.: Fed. Proc., 32: 927, 1973. 26) Alarcon-Segovia, D.: Antibody to nuclear ribonucleoprotein penetrates live human mononuclear cells through Fc receptors. Nature (Lond), 271: 67, 1978. 29) Fiorenzo Paronetto & Emanuel Rubin: Local formation of α-Globulin in the Diseased Liver, and it's Relation to Hepatic Necrosis. Laboratory Investigation, 11 (2), 1962. 1) 藤田実彦:α-fetoproteinに関する臨床病理的研究(I),特に肝障害時の臨床的ならびに基礎的研究.日消誌,71 (11): 1103, 1974. 18) Murgita, R. & Tomasi, T.B. Jr.: Suppression of the immune response by α-fetoprotein. 1.: The effect of mouse α-fetoprotein on the primary and secondary antibody response. J. Exp. Med., 141: 269, 1975. 23) Mizejewsky, G. & Grimley, P.H.: Abortogenic activity of antiserum to alpha-fetoprotein. Nature, 259: 222, 1976. 14) Nishi, S. & Hirai, H.: A new radioimmunoassay of α-fetoprotein and carcinoembryonic antigen. Protides of the Biological Flids 23-rd Collquium. ed. Peters, H., 303, 1976. 24) Rene Hassoux & Jose Uriel.: Abortogenic effect in pregnant rats on autologous antibodies to alpha-fetoprotein. Carcino-Embryonic Proteins, ed. Lehmann, F.G., Vol. 1, 1979. 12) Tatarinov, Tu. S.: Detection of embryospecific α-globulin in the blood sera of patients with primary liver tumor. Vopr. Med. Khim., 10: 90, 1964. 10) Okano, T.: Study of Antigenecity of Human Chorionic Gonadotr opin (hCG). Factors which Effect on hCG immunohistochemistry. Acta Histochem. Cytochem., 13 (4), 1980. 19) Murgita, R. & Tomasi, T.B. Jr.: Suppression of the immune response by α-fetoprotein. II. The effect of mouse α-fetoprotein on mixed lymphocyte transformation. J. Exp. Med., 141: 444, 1975. 5) 佐藤一夫:α-fetoproteinに関する研究(IV),抗AFP血清投与によるラット原発性肝癌の抑制効果に関する研究.日大医誌,37 (12): 1321, 1978. 15) Puck, T.T.: Mammalian cell growth proteins. 1. Growth stimulation by fetuin. Natl. Acad. Sci., 59: 192, 1968. |
| References_xml | – reference: 14) Nishi, S. & Hirai, H.: A new radioimmunoassay of α-fetoprotein and carcinoembryonic antigen. Protides of the Biological Flids 23-rd Collquium. ed. Peters, H., 303, 1976. – reference: 10) Okano, T.: Study of Antigenecity of Human Chorionic Gonadotr opin (hCG). Factors which Effect on hCG immunohistochemistry. Acta Histochem. Cytochem., 13 (4), 1980. – reference: 13) Gold, P. & Freedman, S.O.: Demonstration of tumor specific antigen in human colonic carcinoma by immunological and absorption technique. J. Exp. Med., 121: 439, 1965. – reference: 21) Smith, J.A.: Alpha-fetoprotein; A possible factor necessary for normal development of the embryo. Lancet, 1: 851, 1972. – reference: 29) Fiorenzo Paronetto & Emanuel Rubin: Local formation of α-Globulin in the Diseased Liver, and it's Relation to Hepatic Necrosis. Laboratory Investigation, 11 (2), 1962. – reference: 1) 藤田実彦:α-fetoproteinに関する臨床病理的研究(I),特に肝障害時の臨床的ならびに基礎的研究.日消誌,71 (11): 1103, 1974. – reference: 2) 石塚英夫:α;-fetoproteinに関する研究(II), 3'-Me-DAB肝癌発癌過程の早期におけるα-fetoprotein産生細胞について.日消誌,72 (1): 1224, 1975. – reference: 11) Onoe, T.: Significance of α-fetoprotein appearence in the early stage of azo-dye carcinogenesis. Gann Monograph, 14: 233, 1973. – reference: 27) Alarcon-Segovia, D.A.: Antibody penetration into living cells 1. Intranuclear immunoglobulin in peripheral blood mononuclear cells in mixed connective tissue disease and systemic lupus erythematodes. Clin. Exp. Immunol., 36: 364, 1979. – reference: 19) Murgita, R. & Tomasi, T.B. Jr.: Suppression of the immune response by α-fetoprotein. II. The effect of mouse α-fetoprotein on mixed lymphocyte transformation. J. Exp. Med., 141: 444, 1975. – reference: 4) Fujita, S.: The α-fetoprotein producing cells in the early stage of the experimental liver cancer. Ann. N.Y. Acad. Sci., 259: 217, 1975. – reference: 5) 佐藤一夫:α-fetoproteinに関する研究(IV),抗AFP血清投与によるラット原発性肝癌の抑制効果に関する研究.日大医誌,37 (12): 1321, 1978. – reference: 24) Rene Hassoux & Jose Uriel.: Abortogenic effect in pregnant rats on autologous antibodies to alpha-fetoprotein. Carcino-Embryonic Proteins, ed. Lehmann, F.G., Vol. 1, 1979. – reference: 8) Watabe, H.: Early appearence of embryonic α-globulin in rat serum during carcinogenesis with 4-dimethylaminoazobenzene. Can. Res., 31: 1192, 1971. – reference: 12) Tatarinov, Tu. S.: Detection of embryospecific α-globulin in the blood sera of patients with primary liver tumor. Vopr. Med. Khim., 10: 90, 1964. – reference: 9) Nakane, P.K. & Kawaoi, A.: Peroxidaselabelled antibody. A new method of conjugation. J. Hitochem. Cytochem., 22 (12): 1084, 1974. – reference: 23) Mizejewsky, G. & Grimley, P.H.: Abortogenic activity of antiserum to alpha-fetoprotein. Nature, 259: 222, 1976. – reference: 6) Kaneda, H.: Effect of anti-rat AFP horse serum on the development of hepatoma in the early 3'-Methyl-4-dimethylaminoazobenzene. Carcino-Embryonic Proteins, ed. Lehmann, F.G., Vol. 1, 1979, p.539. – reference: 16) Parmely, M.J.H.F.: Fed. Proc., 32: 927, 1973. – reference: 22) Slade, B.: Antibodies to α-fetoprotein cause fetal mortality in rabbits. Nature, 246: 493, 1973. – reference: 15) Puck, T.T.: Mammalian cell growth proteins. 1. Growth stimulation by fetuin. Natl. Acad. Sci., 59: 192, 1968. – reference: 28) Mori, M. & Novicoff, A.B.: Induction of pinocytosis in rat hepatocytes by partial hepatectomy. J. Cell Biol., 72: 695, 1977. – reference: 3) 上村直也:α-fetoproteinに関する研究(III),特にアゾ色素肝癌における二次反応初期のAFP産生細胞について.日大医誌,38 (3): 595, 1979. – reference: 18) Murgita, R. & Tomasi, T.B. Jr.: Suppression of the immune response by α-fetoprotein. 1.: The effect of mouse α-fetoprotein on the primary and secondary antibody response. J. Exp. Med., 141: 269, 1975. – reference: 7) Tsukada, T. & Mikuni, M.: Effect on antialpha-fetoprotein serum on some cultured tumor cells. Int. J. Cancer, 13: 187, 1974. – reference: 17) Dattwyler, J.R. & Tomasi, T.B.: Inhibition of sensitization of T-cells by alphafetoprotein. Int. J. Cancer, 16: 942, 1975. – reference: 20) Shinzo Nishi,: Species cross-reaction of α-fetoprotein and breakdown of the tolerance to α-fetoprotein by immunization with heterologous α-fetoprotein. Tumor, Res., 8: 17, 1973. – reference: 26) Alarcon-Segovia, D.: Antibody to nuclear ribonucleoprotein penetrates live human mononuclear cells through Fc receptors. Nature (Lond), 271: 67, 1978. – reference: 25) Alarcon-Segovia, D.: Uracil-specific anti-RNA antibodies in scleroderma. Lancet, 1: 363, 1975. |
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| Subtitle | 3'-Me-DABラット肝癌に対する抗AFP抗体投与の効果-その免疫組織化学的検討 |
| Title | α-fetoproteinに関する研究(V) |
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