2-5mmの細胞から採取した卵胞液及びその画分がウシ卯胞卵子の成熟, 受精及び発生に及ぼす影響
2-5mmの牛卵胞から採取した卵胞液あるいはその画分を添加した培地で成熟させた牛卵胞卵子の成熟受精及びその後の胚の発生率に及ぼす影響について検討した. 基本培地 (TCM199+発情牛血清+LH+FSH) に卵胞液を30%添加した区の成熟率及び分割率 (使用卵胞卵子の内, 2細胞期以降へ発達した胚の割合) は, 無添加区 (対照区) より有意に高く, 卵胞液60%添加区の受精率及び分割率も対照区に比較して有意に高い値を示した. また, 卵胞液の高分子画分30%添加区, 低分子画分 (10KD未満) 30%添加区及び低分子画分60%添加区では受精率, 成熟率及び分割率において有意な増加は認められ...
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| Published in | 哺乳動物卵子学会誌 Vol. 11; no. 2; pp. 189 - 195 |
|---|---|
| Main Authors | , |
| Format | Journal Article |
| Language | Japanese |
| Published |
日本哺乳動物卵子学会
01.10.1994
|
| Online Access | Get full text |
| ISSN | 0916-7625 1884-6513 |
| DOI | 10.14893/jmor1991.11.189 |
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| Abstract | 2-5mmの牛卵胞から採取した卵胞液あるいはその画分を添加した培地で成熟させた牛卵胞卵子の成熟受精及びその後の胚の発生率に及ぼす影響について検討した. 基本培地 (TCM199+発情牛血清+LH+FSH) に卵胞液を30%添加した区の成熟率及び分割率 (使用卵胞卵子の内, 2細胞期以降へ発達した胚の割合) は, 無添加区 (対照区) より有意に高く, 卵胞液60%添加区の受精率及び分割率も対照区に比較して有意に高い値を示した. また, 卵胞液の高分子画分30%添加区, 低分子画分 (10KD未満) 30%添加区及び低分子画分60%添加区では受精率, 成熟率及び分割率において有意な増加は認められなかった. また, 高分子画分60%添加区の分割指数 (2細胞期胚の内, それ以降へ発達した胚の割合) は卵胞液60%添加区より有意に低かった. 以上の結果から, 2-5mmの牛卵胞から採取した卵胞液の成熟培地への添加は, 卵胞卵子の成熟, 受精及びその後の胚の発生に有効であるが, その効果は分画により低下することが示唆された. |
|---|---|
| AbstractList | 2-5mmの牛卵胞から採取した卵胞液あるいはその画分を添加した培地で成熟させた牛卵胞卵子の成熟受精及びその後の胚の発生率に及ぼす影響について検討した. 基本培地 (TCM199+発情牛血清+LH+FSH) に卵胞液を30%添加した区の成熟率及び分割率 (使用卵胞卵子の内, 2細胞期以降へ発達した胚の割合) は, 無添加区 (対照区) より有意に高く, 卵胞液60%添加区の受精率及び分割率も対照区に比較して有意に高い値を示した. また, 卵胞液の高分子画分30%添加区, 低分子画分 (10KD未満) 30%添加区及び低分子画分60%添加区では受精率, 成熟率及び分割率において有意な増加は認められなかった. また, 高分子画分60%添加区の分割指数 (2細胞期胚の内, それ以降へ発達した胚の割合) は卵胞液60%添加区より有意に低かった. 以上の結果から, 2-5mmの牛卵胞から採取した卵胞液の成熟培地への添加は, 卵胞卵子の成熟, 受精及びその後の胚の発生に有効であるが, その効果は分画により低下することが示唆された. |
| Author | 前田, 照夫 寺田, 隆登 |
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| References | 7) Sanbuissho, A. and Threlfall, W. R.(1990): The influence of serum and gonadotropins on in vitro maturation and fertilization of bovine oocytes. Theriogenology, 34, 341-348. 14) Rigby, B. R., Ling, S. Y. and Ledwitz-Rigby, F. L.(1983): In search of the elusive follicular factors. In Factors Regulating Ovarian Function (Greenwald, G. S. and Terranova, P. F., eds) p179-183, Raven Press, New York. 13) Ayoub, M. A. and Hunter, A. G.(1993): Inhibitory effect of bovine follicular fluid on in vitro maturation of bovine oocytes. J. Dairy Sci., 76, 95-100. 1) Pincus, G. and Enzmann, E. V.(1935): The comparative behavior of mammalian eggs in vivo and in vitro: 1. The activation of ovarian eggs. J. Exp. Med., 62, 655-675. 12) Leibfried, L. and First, N. L.(1980): Effect of bovine and porcine follicular fluid and granulosa cells on maturation of oocytes in vitro. Biol. Reprod., 23, 699-704. 4) Leibfried-Rutledge, M. L., Critser, E. S., Eyestone, W. H., Northy, D. L. and First, N. L.(1987): Development potential of bovine oocytes matured in vitro or in vivo. Biol. Reprod., 36, 376-383. 11) Yoshida, M., Ishizaki, Y., Kawagishi, H., Bamba K. and Kojima, Y.(1992): Effects of pig follicular fluid on maturation of pig oocytes in vitro and on their subsequent fertilizing and developmental capacity in vitro. J. Reprod. Fert., 95, 481-488. 3) Thibault, C.(1977): Are folicular maturation and oocyte maturation independent processes? J. Reprod. Fert., 51, 1-15. 6) Younis, A. I., Brackett, B. G. and Fayrer-Hosken, R. A.(1989): Influence of serum and hormone on bovine oocyte maturation and fertilization in vitro. Gamete Res., 23, 189-201. 8) Leibfried, L. and First, N. L.(1979): Characterization of bovine follicular oocytes and their ability to mature in vitro. J. Anim. Sci., 48, 76-86. 9) Parrish, J. J., Susko-Parrish, J., Winer, M. A. and First, N. L.(1988): Capacitation of bovine sperm by heparin. Biol. Reprod., 38, 1171-1180. 2) Niwa, K. and Chang, M. C.(1975): Fertilization of rat eggs in vitro at various times before and after ovulation with special reference to fertilization of ovarian oocytes matured in culture. J. Reprod. Fert., 43, 435-451. 10) Sokal, R. R. and Rohlf, F. J.(1973): Introduction to bi ostatistics. W. H. Freeman and Company, San Francisco and London, 5-19. 5) Moor, R. M. and Trounson, A. O.(1977): Hormonal and follicular factors affecting maturation of sheep oocytes in vitro and their subsequent developmental capacity. J. Reprod. Fert., 49, 101-109. |
| References_xml | – reference: 2) Niwa, K. and Chang, M. C.(1975): Fertilization of rat eggs in vitro at various times before and after ovulation with special reference to fertilization of ovarian oocytes matured in culture. J. Reprod. Fert., 43, 435-451. – reference: 3) Thibault, C.(1977): Are folicular maturation and oocyte maturation independent processes? J. Reprod. Fert., 51, 1-15. – reference: 10) Sokal, R. R. and Rohlf, F. J.(1973): Introduction to bi ostatistics. W. H. Freeman and Company, San Francisco and London, 5-19. – reference: 11) Yoshida, M., Ishizaki, Y., Kawagishi, H., Bamba K. and Kojima, Y.(1992): Effects of pig follicular fluid on maturation of pig oocytes in vitro and on their subsequent fertilizing and developmental capacity in vitro. J. Reprod. Fert., 95, 481-488. – reference: 9) Parrish, J. J., Susko-Parrish, J., Winer, M. A. and First, N. L.(1988): Capacitation of bovine sperm by heparin. Biol. Reprod., 38, 1171-1180. – reference: 12) Leibfried, L. and First, N. L.(1980): Effect of bovine and porcine follicular fluid and granulosa cells on maturation of oocytes in vitro. Biol. Reprod., 23, 699-704. – reference: 7) Sanbuissho, A. and Threlfall, W. R.(1990): The influence of serum and gonadotropins on in vitro maturation and fertilization of bovine oocytes. Theriogenology, 34, 341-348. – reference: 8) Leibfried, L. and First, N. L.(1979): Characterization of bovine follicular oocytes and their ability to mature in vitro. J. Anim. Sci., 48, 76-86. – reference: 1) Pincus, G. and Enzmann, E. V.(1935): The comparative behavior of mammalian eggs in vivo and in vitro: 1. The activation of ovarian eggs. J. Exp. Med., 62, 655-675. – reference: 4) Leibfried-Rutledge, M. L., Critser, E. S., Eyestone, W. H., Northy, D. L. and First, N. L.(1987): Development potential of bovine oocytes matured in vitro or in vivo. Biol. Reprod., 36, 376-383. – reference: 14) Rigby, B. R., Ling, S. Y. and Ledwitz-Rigby, F. L.(1983): In search of the elusive follicular factors. In Factors Regulating Ovarian Function (Greenwald, G. S. and Terranova, P. F., eds) p179-183, Raven Press, New York. – reference: 5) Moor, R. M. and Trounson, A. O.(1977): Hormonal and follicular factors affecting maturation of sheep oocytes in vitro and their subsequent developmental capacity. J. Reprod. Fert., 49, 101-109. – reference: 13) Ayoub, M. A. and Hunter, A. G.(1993): Inhibitory effect of bovine follicular fluid on in vitro maturation of bovine oocytes. J. Dairy Sci., 76, 95-100. – reference: 6) Younis, A. I., Brackett, B. G. and Fayrer-Hosken, R. A.(1989): Influence of serum and hormone on bovine oocyte maturation and fertilization in vitro. Gamete Res., 23, 189-201. |
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| Title | 2-5mmの細胞から採取した卵胞液及びその画分がウシ卯胞卵子の成熟, 受精及び発生に及ぼす影響 |
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