STUDIES ON GIARDIASIS
Many reports on Giardia lamblia have been published since it was discovered by Lambl in 1859. However the pathogenesis of this disease has not been made clear because the pure culture of lamblia is not possible. The vegetative form of this protozoa was found in the duodenal contents of two patients...
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| Published in | Nihon Naika Gakkai Zasshi Vol. 47; no. 10; pp. 1300 - 1311 |
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| Main Author | |
| Format | Journal Article |
| Language | Japanese |
| Published |
The Japanese Society of Internal Medicine
10.01.1959
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| Online Access | Get full text |
| ISSN | 0021-5384 1883-2083 |
| DOI | 10.2169/naika.47.1300 |
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| Abstract | Many reports on Giardia lamblia have been published since it was discovered by Lambl in 1859. However the pathogenesis of this disease has not been made clear because the pure culture of lamblia is not possible. The vegetative form of this protozoa was found in the duodenal contents of two patients who were initially diagnosed as having cholecystitis. Clinical considerations of the pathogenesis were carried out in this case. In the lst case, therapy consisted of oral administration of “Resochin” (Chloroquine-diphosphate) which showed good results. In an effort to investigate the pathogenesis of this disease, an attempt at pure culture was made using the vegetative form obtained from these patients. Five culture mediums tried were the modified V-Bouillon which I called L1-5 Bouillon. Following results were obtained: 1) The optimum pH of the medium for culture was found to be at 6.4. 2) Bouillons containing various concentrations of pig liver were tried and it was found that L4-Bouillon which contained 15 grams of pig liver per 100ml of water was the most suitable for culture. Distinct movement of the lamblia was observed for 12 days and on the second to the fourth day reproduction and multiplication-binary longitudinal fusion-of the vegetative form of the lamblia were seen. 3) As for the effect of antibiotics used in the culture solution to inhibit the growth of bacteria colimycin proved better than streptomycin. 4) Reproduction and multiplication of the lamblia were observed and its mode of reproduction was described. As noted above, I succeeded in culturing the lamblia for 12 days. However, the pure and continuous culture of this protozoa has not yet been accomplished. Further efforts are being made to study the pathogenesis of this disease. |
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| AbstractList | Many reports on Giardia lamblia have been published since it was discovered by Lambl in 1859. However the pathogenesis of this disease has not been made clear because the pure culture of lamblia is not possible. The vegetative form of this protozoa was found in the duodenal contents of two patients who were initially diagnosed as having cholecystitis. Clinical considerations of the pathogenesis were carried out in this case. In the lst case, therapy consisted of oral administration of “Resochin” (Chloroquine-diphosphate) which showed good results. In an effort to investigate the pathogenesis of this disease, an attempt at pure culture was made using the vegetative form obtained from these patients. Five culture mediums tried were the modified V-Bouillon which I called L1-5 Bouillon. Following results were obtained: 1) The optimum pH of the medium for culture was found to be at 6.4. 2) Bouillons containing various concentrations of pig liver were tried and it was found that L4-Bouillon which contained 15 grams of pig liver per 100ml of water was the most suitable for culture. Distinct movement of the lamblia was observed for 12 days and on the second to the fourth day reproduction and multiplication-binary longitudinal fusion-of the vegetative form of the lamblia were seen. 3) As for the effect of antibiotics used in the culture solution to inhibit the growth of bacteria colimycin proved better than streptomycin. 4) Reproduction and multiplication of the lamblia were observed and its mode of reproduction was described. As noted above, I succeeded in culturing the lamblia for 12 days. However, the pure and continuous culture of this protozoa has not yet been accomplished. Further efforts are being made to study the pathogenesis of this disease. |
| Author | Araki, Tsuneji |
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| DOI | 10.2169/naika.47.1300 |
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| References | 7) Tsuchiya, H.: Am. J. Hyg., 13, 544, 1931. 1) Strong: Stitt's diagnosis, prevention and treatment of tropical disease. Blakiston, 1943. 4) Véghelyi, P.: Am. J. Dis. Child, 57, 894, 1939. 5) Simon, C. E.: Am. J. Hyg., 1, 440, 1921. 12) 野木一雄:綜合臨床, 1, (5), 472,昭和27年. 10) Strong: Stitt's Diagnosis, prevention and treatment of tropical disease. Blakiston, 1943. 10) 浜田義雄:大阪大学医学雑誌,5, (5) 429,昭和28年. 9) 中村敬三,秋葉朝一郎編:細菌学各論II,南山堂,昭和30年. 2) Belding: Textbook of Clinical parasitology. Appleton-Century-Crofts, 1952. 2) 卜部昭他:日本寄生虫学会記事, 20, 85,昭和26年. (1951). 5) Véghelyi, P.: Am. J. Dis. Child, 58, 793, 1940. 6) Tsuchiya, H.: Am. J. Hyg., 12, 467, 500, 1930. 14) 内藤益一:実験消化機病学, 9, (4), 580,昭和9年. 8) Papke, W.: Dtsch. Med. Wschr., 66, 629, 1940. 13) Manson-Bahr, P.: The Dysenteric Disorders, London, 1939. 3) Véghelyi, P.: Am, J.Dis. Child, 56, 1231, 1938. 15) 増山善明他:臨床内科小児科, 11, (7), 461,昭和31年. 16) 鮒子田繁雄他:臨床内科小児科, 11 (10), 711,昭和31年. 11) Calder R.M. & Rigdon R.H.: Am. J. M. Sc. 190, 82, 1935. 1) 野崎恭勝:医療, 10, (5) 401,昭和31年. 8) Hegner, R. W.: Am. J. Hyg., 7, 782, 1927. 9) Armaghan, V.: Am. J. Hyg., 26, 236, 1937. 4) 野崎恭勝:医療, 10, (5) 401,昭和31年. 3) 中村敬三,秋葉朝一郎編:細菌学各論II.南山堂,昭和30年. 6) De Muro, P.: Acta Med. Scandinavia, 103, 17, 1939. 7) 吉田邦男:小児科診療, 16 (8), 529,昭和28年. |
| References_xml | – reference: 16) 鮒子田繁雄他:臨床内科小児科, 11 (10), 711,昭和31年. – reference: 14) 内藤益一:実験消化機病学, 9, (4), 580,昭和9年. – reference: 12) 野木一雄:綜合臨床, 1, (5), 472,昭和27年. – reference: 4) Véghelyi, P.: Am. J. Dis. Child, 57, 894, 1939. – reference: 2) 卜部昭他:日本寄生虫学会記事, 20, 85,昭和26年. (1951). – reference: 4) 野崎恭勝:医療, 10, (5) 401,昭和31年. – reference: 5) Simon, C. E.: Am. J. Hyg., 1, 440, 1921. – reference: 7) Tsuchiya, H.: Am. J. Hyg., 13, 544, 1931. – reference: 7) 吉田邦男:小児科診療, 16 (8), 529,昭和28年. – reference: 10) 浜田義雄:大阪大学医学雑誌,5, (5) 429,昭和28年. – reference: 8) Hegner, R. W.: Am. J. Hyg., 7, 782, 1927. – reference: 6) Tsuchiya, H.: Am. J. Hyg., 12, 467, 500, 1930. – reference: 10) Strong: Stitt's Diagnosis, prevention and treatment of tropical disease. Blakiston, 1943. – reference: 9) Armaghan, V.: Am. J. Hyg., 26, 236, 1937. – reference: 9) 中村敬三,秋葉朝一郎編:細菌学各論II,南山堂,昭和30年. – reference: 2) Belding: Textbook of Clinical parasitology. Appleton-Century-Crofts, 1952. – reference: 1) 野崎恭勝:医療, 10, (5) 401,昭和31年. – reference: 3) 中村敬三,秋葉朝一郎編:細菌学各論II.南山堂,昭和30年. – reference: 11) Calder R.M. & Rigdon R.H.: Am. J. M. Sc. 190, 82, 1935. – reference: 5) Véghelyi, P.: Am. J. Dis. Child, 58, 793, 1940. – reference: 3) Véghelyi, P.: Am, J.Dis. Child, 56, 1231, 1938. – reference: 15) 増山善明他:臨床内科小児科, 11, (7), 461,昭和31年. – reference: 13) Manson-Bahr, P.: The Dysenteric Disorders, London, 1939. – reference: 1) Strong: Stitt's diagnosis, prevention and treatment of tropical disease. Blakiston, 1943. – reference: 8) Papke, W.: Dtsch. Med. Wschr., 66, 629, 1940. – reference: 6) De Muro, P.: Acta Med. Scandinavia, 103, 17, 1939. |
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| Snippet | Many reports on Giardia lamblia have been published since it was discovered by Lambl in 1859. However the pathogenesis of this disease has not been made clear... |
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| Title | STUDIES ON GIARDIASIS |
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