Cytoplasmic blood plasma inclusions of hepatocytes associated with idiopathic portal hypertension-report of a case
A case with cytoplasmic inclusions composed of blood plasma elements was reported. The patient was 9-year-old boy, who showed splenomegaly and esophageal varices with portal hypertension. Light microscopic examination of the biopsied liver revealed cytoplasmic eosinophilic inclusions in marked swell...
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| Published in | Kanzo Vol. 21; no. 10; pp. 1358 - 1365 |
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| Main Authors | , , , , , , , , , |
| Format | Journal Article |
| Language | Japanese |
| Published |
The Japan Society of Hepatology
25.10.1980
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| Online Access | Get full text |
| ISSN | 0451-4203 1881-3593 |
| DOI | 10.2957/kanzo.21.1358 |
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| Abstract | A case with cytoplasmic inclusions composed of blood plasma elements was reported. The patient was 9-year-old boy, who showed splenomegaly and esophageal varices with portal hypertension. Light microscopic examination of the biopsied liver revealed cytoplasmic eosinophilic inclusions in marked swelling hepatocytes. The inclusions were numerously scattered throughout liver lobules, mostly round in shape, variable in size, measuring 3μ to 20μ in diameter, and positive for diastase rcsistent PAS. It revcaled that most of the inclusions were positive for such blood plasma components as albumin, IgG, IgM, IgA, transferrin and fibrinogen by immunoperoxidase method. These inclusions are usually observed in the state of increase of intrasinusoidal blood pressure. Sclerosis of central vein and perisinusoidal fibrosis also noted. Thus, it was postulated that portal hypertension of this case was caused mainly by increase of intrasinusoidal blood pressure. To our knowledge, the present case represents the first report in which the exact nature of cytoplasmic blood plasma inclusions in human hepatocytes has been demonstrated by the technique of the immunoperoxidase method. |
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| AbstractList | A case with cytoplasmic inclusions composed of blood plasma elements was reported. The patient was 9-year-old boy, who showed splenomegaly and esophageal varices with portal hypertension. Light microscopic examination of the biopsied liver revealed cytoplasmic eosinophilic inclusions in marked swelling hepatocytes. The inclusions were numerously scattered throughout liver lobules, mostly round in shape, variable in size, measuring 3μ to 20μ in diameter, and positive for diastase rcsistent PAS. It revcaled that most of the inclusions were positive for such blood plasma components as albumin, IgG, IgM, IgA, transferrin and fibrinogen by immunoperoxidase method. These inclusions are usually observed in the state of increase of intrasinusoidal blood pressure. Sclerosis of central vein and perisinusoidal fibrosis also noted. Thus, it was postulated that portal hypertension of this case was caused mainly by increase of intrasinusoidal blood pressure. To our knowledge, the present case represents the first report in which the exact nature of cytoplasmic blood plasma inclusions in human hepatocytes has been demonstrated by the technique of the immunoperoxidase method. |
| Author | ABE, Kenji OKANO, Tadao SUHARA, Yutaka SAITO, Momoko SHIKATA, Toshio WAKU, Yoji INAMI, Makoto OWADA, Misao SUZUKI, Yuji SHIONOME, Shoichi |
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| References | 18) 下田敏彦他:本態不明の細胞質内好酸球状体を多数の肝細胞に認めた慢性肝炎の一剖検例.肝臓,20:189, 1979. 2) 鈴木忠彦:門脈圧亢進症-成因と病理-.日外会誌,57:987, 1956. 27) Goldfisher, S.: Hemoglobin uptake by rat hepatocytes and its breakdown with lysosomes. J. Cell Biol., 44:513, 1970. 11) 柄沢勉他:エタノール投与チンパンジー肝にみられた球状巨大糸粒体.肝臓,20:620, 1979. 24) Fisher, E.R. & Fisher, B.: Cytoplasmic liver cell inclusions following arterialization in the dog. Am. J. Pathol., 30:987, 1954. 17) Reintoft, I.: Periodic acid Schiff-positive nonglycogenic globules in hepatccytes. Acta. Path. Microbiol. Scand. Sect. A, 86:325, 1978. 14) Palmer, P.E. & Wolfe, H.J.: α1-antitrypsin deposition in primary hepatic carcinomas. Arch. Path., 100:232, 1976. 20) Price, J.M. & Laird, A.K.: A comparison of the intercelullar composition of regenerating liver and induced liver tumors. Cancer Res., 10:650, 1950. 5) 志方俊夫,柄沢勉:特発性門脈圧亢進症に於ける肝内異常血行路の研究.肝臓,18:814, 1977. 15) DeLellis, R.A.: Distinctive hepatic cell globules im adult alpha-1-antitrypsin dificiency. Arch. Path., 94:308, 1972. 7) Abe, K.: Cytoplasmic blood plasma inclusions of canine hepatocytes demonstration by immunoperoxidase-labelling method. Am. J. Vet. Res., 41:1507, 1980. 25) 梶原博毅他:急性うっ血肝に於ける肝細胞内封入体に関する組織化学的,電顕的研究.日病会誌(総会号),66:94, 1977. 21) Doniach, I. & Weinbren, K.: The development of inclusion bodies in the cells of the rat' liver after partial hepatectomy. Brit. J. Exp. Pathol., 33:499, 1952. 6) 阿部賢治他:肝細胞質内血漿性封入体一犬に於ける自然発生例-.肝臓,21; 282, 1980. 8) Nakane, P.K. & Kawaoi, A.: PeroxidagseIabeled antibody. A new method of conjugation. J. Histochem. Cytochem., 22:1084, 1974. 16) Fisher, R.L.: α-1-antitrypsin dificiency im liver disease: The extent of the problem. Gastroenterology, 71:646, 1976. 3) 山本貞博:特発性門脈高圧症.(2)肝内門脈閉塞症:肝内循環の動態より.日内会誌,57: 1312, 1968. 19) Paronetto, F.: Local formation of yglobulin in the diseased liver, and its relation to hepatic necrosis. Lab. Invest., 11:150, 1962. 13) Norkin, S.A. & Campagna-pinto, D.: Cytoplasmic hyaline inclusions in hepatoma. Arch. Path., 86:25, 1968. 9) Bruguera, M.: Giant mitochondria in hepatocytes. Gastroenterology, 73:1383, 1977. 12) Ishak, K. & Glunz, P.R.: Hepatoblastoma and hepatocarcinoma in infancy and childhood. Cancer, 20:396, 1967. 1) 佐藤寿雄他:バンチ症候群のうっ血脾腫論に対する疑問.日本医事新報,2238:48, 1967. 26) Rosin, A. & Doljanski, L.: Erythrocytes in cytoplasma and nuclei of liver cells. Brit. J. Exp. Pathol., 25:111, 1944. 22) Pfeifer, U. & Bannasch, P.: The problem of "hyalin protein droplets" in the cytoplasm of liver cells. Virchows Arch. Abt. B Zellpath., 1:365, 1968. 4) 中田勝次,矢田博之:特発性門脈圧亢進症における肝の病理組織学的所見.特発性門脈圧亢進症調査研究班,昭和50年度研究報告書,p.25. 10) Koch, O.R.: Ultrastructural and biochemical aspects of liver mitochondria during rccovery from ethanol-induced alterlation. Am. J. Pathol., 90:325, 1978. 23) Mori, M. & Novikoff, A.B.: Induction of pinocytosis in rat hepatocytes by partial hepatectomy. J. Cell Biol., 72:695, 1977. |
| References_xml | – reference: 17) Reintoft, I.: Periodic acid Schiff-positive nonglycogenic globules in hepatccytes. Acta. Path. Microbiol. Scand. Sect. A, 86:325, 1978. – reference: 21) Doniach, I. & Weinbren, K.: The development of inclusion bodies in the cells of the rat' liver after partial hepatectomy. Brit. J. Exp. Pathol., 33:499, 1952. – reference: 1) 佐藤寿雄他:バンチ症候群のうっ血脾腫論に対する疑問.日本医事新報,2238:48, 1967. – reference: 4) 中田勝次,矢田博之:特発性門脈圧亢進症における肝の病理組織学的所見.特発性門脈圧亢進症調査研究班,昭和50年度研究報告書,p.25. – reference: 16) Fisher, R.L.: α-1-antitrypsin dificiency im liver disease: The extent of the problem. Gastroenterology, 71:646, 1976. – reference: 5) 志方俊夫,柄沢勉:特発性門脈圧亢進症に於ける肝内異常血行路の研究.肝臓,18:814, 1977. – reference: 9) Bruguera, M.: Giant mitochondria in hepatocytes. Gastroenterology, 73:1383, 1977. – reference: 27) Goldfisher, S.: Hemoglobin uptake by rat hepatocytes and its breakdown with lysosomes. J. Cell Biol., 44:513, 1970. – reference: 8) Nakane, P.K. & Kawaoi, A.: PeroxidagseIabeled antibody. A new method of conjugation. J. Histochem. Cytochem., 22:1084, 1974. – reference: 2) 鈴木忠彦:門脈圧亢進症-成因と病理-.日外会誌,57:987, 1956. – reference: 26) Rosin, A. & Doljanski, L.: Erythrocytes in cytoplasma and nuclei of liver cells. Brit. J. Exp. Pathol., 25:111, 1944. – reference: 15) DeLellis, R.A.: Distinctive hepatic cell globules im adult alpha-1-antitrypsin dificiency. Arch. Path., 94:308, 1972. – reference: 13) Norkin, S.A. & Campagna-pinto, D.: Cytoplasmic hyaline inclusions in hepatoma. Arch. Path., 86:25, 1968. – reference: 22) Pfeifer, U. & Bannasch, P.: The problem of "hyalin protein droplets" in the cytoplasm of liver cells. Virchows Arch. Abt. B Zellpath., 1:365, 1968. – reference: 25) 梶原博毅他:急性うっ血肝に於ける肝細胞内封入体に関する組織化学的,電顕的研究.日病会誌(総会号),66:94, 1977. – reference: 23) Mori, M. & Novikoff, A.B.: Induction of pinocytosis in rat hepatocytes by partial hepatectomy. J. Cell Biol., 72:695, 1977. – reference: 6) 阿部賢治他:肝細胞質内血漿性封入体一犬に於ける自然発生例-.肝臓,21; 282, 1980. – reference: 18) 下田敏彦他:本態不明の細胞質内好酸球状体を多数の肝細胞に認めた慢性肝炎の一剖検例.肝臓,20:189, 1979. – reference: 20) Price, J.M. & Laird, A.K.: A comparison of the intercelullar composition of regenerating liver and induced liver tumors. Cancer Res., 10:650, 1950. – reference: 19) Paronetto, F.: Local formation of yglobulin in the diseased liver, and its relation to hepatic necrosis. Lab. Invest., 11:150, 1962. – reference: 11) 柄沢勉他:エタノール投与チンパンジー肝にみられた球状巨大糸粒体.肝臓,20:620, 1979. – reference: 12) Ishak, K. & Glunz, P.R.: Hepatoblastoma and hepatocarcinoma in infancy and childhood. Cancer, 20:396, 1967. – reference: 14) Palmer, P.E. & Wolfe, H.J.: α1-antitrypsin deposition in primary hepatic carcinomas. Arch. Path., 100:232, 1976. – reference: 10) Koch, O.R.: Ultrastructural and biochemical aspects of liver mitochondria during rccovery from ethanol-induced alterlation. Am. J. Pathol., 90:325, 1978. – reference: 7) Abe, K.: Cytoplasmic blood plasma inclusions of canine hepatocytes demonstration by immunoperoxidase-labelling method. Am. J. Vet. Res., 41:1507, 1980. – reference: 3) 山本貞博:特発性門脈高圧症.(2)肝内門脈閉塞症:肝内循環の動態より.日内会誌,57: 1312, 1968. – reference: 24) Fisher, E.R. & Fisher, B.: Cytoplasmic liver cell inclusions following arterialization in the dog. Am. J. Pathol., 30:987, 1954. |
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| Title | Cytoplasmic blood plasma inclusions of hepatocytes associated with idiopathic portal hypertension-report of a case |
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