Studies can Treatment with Antitumor Agents Effects of Antitumor Agents to KB Cell in Vitro in Short Time

• Published in: Japanese Journal of National Medical Services Vol. 21; no. 10; pp. 1183 - 1192
• Main Authors: KATSUTA, Keiichi, HISATSUNE, Kazuo, KAJIYAMA, Minoru, MIYAHARA, Hiroji, ATSUMI, Takeshige, DOJYO, Masahiro
• Format: Journal Article
• Language: Japanese
• Published: Japanese Society of National Medical Services 20.10.1967
• ISSN: 0021-1699; 1884-8729
• DOI: 10.11261/iryo1946.21.1183

After combining several antitumor agents such as Nitrogen mustard N-oxide, Cyclophosphamide, Mitomycin C, and Chromomycin A8 with LY culture media containing 20% inactivated bovine serum with KB cells for 30 or 60 minutes once at 37°C, the antitumor agents were washed out with Hanks' BSS, then new LY culture medium was added to the cell, and kept at 37°C for 1-7 days. The effects of antitumor agents upon cell number, mitotic rate, and morphologic changes were studied. The results obtained were as follows 1) Moderate inhibition of cell growth of KB cell was seen after combining either 1 mcg/ml or 1Omcg/ml of Nitrogen mustard N-oxide with the cells. 2) Very minor degree of inhibition of cell growth of KB cells was noted if either 2mcg/ml or 20mcg/ml of Cyclophamide was added to the cells. 3) Mitomycin C revealed moderate degree of inhibition of cell growth 2-5 days after combining either 0.04mcg/ml or 0.4mcg/ml of the drug with KB cell. Re-increase in mitotic rate, however, was seen after a days in the former experimental series despite of no increase in mitotic rate in the latter series. 4) Similar results to those in the experimental series of Mitomycin C were observed in the series of 0.01mcg/ml and 0.1mcg/ml of Chromomycin A3.