In Vitro Fertilization of Bovine Oocytes With Serono Gamete Preparation Medium (GPM)
The purpose of the present study was to investigate the efficacy of Serono Gamete Preparation Medium (GPM), which is used as the medium for human in vitro fertilization, as the fertilization medium for bovine oocytes matured in vitro. Oocytes were inseminated in GPM or GPM supplemented with 5mM caff...
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| Published in | Journal of Mammalian Ova Research Vol. 11; no. 2; pp. 196 - 202 |
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| Main Authors | , , , , , |
| Format | Journal Article |
| Language | Japanese |
| Published |
Japanese Society of Mammalian Ova Research
01.10.1994
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| Subjects | |
| Online Access | Get full text |
| ISSN | 0916-7625 1884-6513 |
| DOI | 10.14893/jmor1991.11.196 |
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| Abstract | The purpose of the present study was to investigate the efficacy of Serono Gamete Preparation Medium (GPM), which is used as the medium for human in vitro fertilization, as the fertilization medium for bovine oocytes matured in vitro. Oocytes were inseminated in GPM or GPM supplemented with 5mM caffeine and 10μg/ml heparin (GPM-CH). Brackett and Oliphant's (BO) medium supplemented with 5mM caffeine and 10μg/ml heparin (BO-CH) was used as a control medium. There were no differences in the rates of cleaved embryos and development to blastocysts between GPM-CH and BO-CH (72.0% vs 65.0%, 22.0% vs 25.0%, respectively). However, the rates were significantly low when oocytes were inseminated in GPM alone. It was found that there were no differences in the rates of development to blastocysts by using frozen spermatozoa derived from two different bulls when GPM-CH was used. These results indicated that GPM was successfully used as the fertilization medium for bovine oocytes. |
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| AbstractList | The purpose of the present study was to investigate the efficacy of Serono Gamete Preparation Medium (GPM), which is used as the medium for human in vitro fertilization, as the fertilization medium for bovine oocytes matured in vitro. Oocytes were inseminated in GPM or GPM supplemented with 5mM caffeine and 10μg/ml heparin (GPM-CH). Brackett and Oliphant's (BO) medium supplemented with 5mM caffeine and 10μg/ml heparin (BO-CH) was used as a control medium. There were no differences in the rates of cleaved embryos and development to blastocysts between GPM-CH and BO-CH (72.0% vs 65.0%, 22.0% vs 25.0%, respectively). However, the rates were significantly low when oocytes were inseminated in GPM alone. It was found that there were no differences in the rates of development to blastocysts by using frozen spermatozoa derived from two different bulls when GPM-CH was used. These results indicated that GPM was successfully used as the fertilization medium for bovine oocytes. |
| Author | SHINTANI, Yoshio MAEDA, Jun-ichi KITAMURA, Toru SASAKI, Kiyotaka KOBAYASHI, Syuichi SAKAI, Susumu |
| Author_xml | – sequence: 1 fullname: SAKAI, Susumu organization: Fukui Prefectural Livestock Experiment Station – sequence: 1 fullname: KITAMURA, Toru organization: Fukui Prefectural Livestock Experiment Station – sequence: 1 fullname: KOBAYASHI, Syuichi organization: Fukui Prefectural Livestock Experiment Station – sequence: 1 fullname: SHINTANI, Yoshio organization: Fukui Prefectural Livestock Experiment Station – sequence: 1 fullname: SASAKI, Kiyotaka organization: Fukui Prefectural Livestock Hygiene Center – sequence: 1 fullname: MAEDA, Jun-ichi organization: Fukui Prefectural Livestock Experiment Station |
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| References | 4) Utsumi, K., Kato, H. and Iritani, A.(1991): Full-term development of bovine follicular oocytes matured in culture and fertilized in vitro. Theriogenology, 35, 695-703. 2) Kajihara, Y., Goto, K., Kosaka, S., Nakanishi, Y. and Ogawa, K.(1987): In vitro fertilization of bovine follicular oocytes and their development up to hatched blastocysts in vitro. Jpn. J. Anim. Reprod., 33, 173-180 (in Japanese). 15) Parrish, J. J., Susko-Parrish, J. L., and First, N. L.(1985): Effect of heparin and chondroitin sulfate on the acrosome reaction and fertility of bovine sperm in vitro. Theriogenology, 24, 537-549. 1) Hanada, A., Shioya, Y. and Suzuki, T.(1986): Birth of calves from non-surgical transfer of blastocyst originated from in vitro fertilized oocytes matured in vitro. Proceedings of the 78th Meeting of the Japanese Society of Zootechnical Science, I-35, p-18 (in Japanese). 9) Chian, R. C., Nakahara, H., Niwa, K. and Funahashi, H.(1992): Fertilization and early cleavage in vitro of ageing bovine oocytes after maturation in culture. Theriogenology, 37, 665-672. 11) Araki, S., Motoyama, M., Sato, T., Sayama, M., Akahori, A., Tamada, T.(1990): Active sperm separation method used new culture medium GPM for intrafallopian insemination, in vitro fertilization and gamete intrafallopian transfer. Jap. J. Cli. Gyne. Obster., 44, 708-712 (in Japanese). 16) Biggers, J. D., Whitten, W. K.& Whittingham, D. G.(1971): Methods in Mammalian embryology (Daniel, J. C., ed.), p86-116, W. H. Freeman and Company, San Francisco. 5) Brackett, B. G. and Oliphant, G.(1975): Capacitation of rabbit spermatozoan vitro. Biol. Reprod., 12, 260-274. 17) Goto, K., Takuma, Y., Ooe, N. and Ogawa, K.(1990): In vitro development of bovine oocytes collected from ovaries of individual cows after in vitro fertilization. Jpn. J. Anim. Reprod., 36, 110-113 (in Japanese). 8) Kim, C. I., Ellington, J. E. and Foote, R. H.(1990): Maturation, fertilization and development of bovine oocytes in vitro using TCM 199 and a simple defined medium with co-culture. Theriogenology, 33, 433-440. 14) Khan, I., Staessen, C., Devroey, P., Van Steirteghem, A. C.(1991): Human serum albumin versus serum: a comparative study on embryo transfer medium. Fertil. Steril., 56, 98-101. 13) Maeda, J., Negami, A, I., Kobayashi, S. and Tominaga, T.(1992): Effects of FSH on maturation of fertilized bovine oocytes in vitro. J. Fertil. Implant. 9, 197-200 (in Japanese). 7) Parrish, J. J., Susko-Parrish, J., Winer, M. A. and First, N. L.(1988): Capacitation of bovine sperm by heparin. Biol. Reprod., 38, 1771-1180. 12) Gadd, S. C., Jenki ns, J. M., Davies, D. W., Anthony, F. W. and Mass on, G. M.(1990): Comparison of Serono Gamete Preparation Medium (GPM) with Earle' s balanced salt solution (EBSS) for in-vitro fertilization (IVF).Conceive, 19, 10-11. 10) Nakao, H. and Nakatsuji, N.(1990): Effects of co-culture, medium components and gas phase on in vitro culture of in vitro matured and in vitro fertilized bovine embryos. Theriogenology, 33, 591-600. 6) Bavister, B. D. and Yanagimachi, R.(1977): The effects of sperm extract and energy sources on the motility and acrosome reaction of hamster sperm in vitro. Biol. Reprod., 16, 228-237. 3) Goto, K., Kajihara, Y., Kosaka, S., Koba, M., Nakanishi, Y. and Ogawa, K.(1988) Pregnancies after co-culture of cumulus cells with bovine embryos derived from in-vitro fertilization of in-vitro matured follicular oocytes. J. Reprod. Fertil., 83, 753-758. |
| References_xml | – reference: 12) Gadd, S. C., Jenki ns, J. M., Davies, D. W., Anthony, F. W. and Mass on, G. M.(1990): Comparison of Serono Gamete Preparation Medium (GPM) with Earle' s balanced salt solution (EBSS) for in-vitro fertilization (IVF).Conceive, 19, 10-11. – reference: 17) Goto, K., Takuma, Y., Ooe, N. and Ogawa, K.(1990): In vitro development of bovine oocytes collected from ovaries of individual cows after in vitro fertilization. Jpn. J. Anim. Reprod., 36, 110-113 (in Japanese). – reference: 3) Goto, K., Kajihara, Y., Kosaka, S., Koba, M., Nakanishi, Y. and Ogawa, K.(1988) Pregnancies after co-culture of cumulus cells with bovine embryos derived from in-vitro fertilization of in-vitro matured follicular oocytes. J. Reprod. Fertil., 83, 753-758. – reference: 1) Hanada, A., Shioya, Y. and Suzuki, T.(1986): Birth of calves from non-surgical transfer of blastocyst originated from in vitro fertilized oocytes matured in vitro. Proceedings of the 78th Meeting of the Japanese Society of Zootechnical Science, I-35, p-18 (in Japanese). – reference: 4) Utsumi, K., Kato, H. and Iritani, A.(1991): Full-term development of bovine follicular oocytes matured in culture and fertilized in vitro. Theriogenology, 35, 695-703. – reference: 5) Brackett, B. G. and Oliphant, G.(1975): Capacitation of rabbit spermatozoan vitro. Biol. Reprod., 12, 260-274. – reference: 9) Chian, R. C., Nakahara, H., Niwa, K. and Funahashi, H.(1992): Fertilization and early cleavage in vitro of ageing bovine oocytes after maturation in culture. Theriogenology, 37, 665-672. – reference: 10) Nakao, H. and Nakatsuji, N.(1990): Effects of co-culture, medium components and gas phase on in vitro culture of in vitro matured and in vitro fertilized bovine embryos. Theriogenology, 33, 591-600. – reference: 16) Biggers, J. D., Whitten, W. K.& Whittingham, D. G.(1971): Methods in Mammalian embryology (Daniel, J. C., ed.), p86-116, W. H. Freeman and Company, San Francisco. – reference: 13) Maeda, J., Negami, A, I., Kobayashi, S. and Tominaga, T.(1992): Effects of FSH on maturation of fertilized bovine oocytes in vitro. J. Fertil. Implant. 9, 197-200 (in Japanese). – reference: 14) Khan, I., Staessen, C., Devroey, P., Van Steirteghem, A. C.(1991): Human serum albumin versus serum: a comparative study on embryo transfer medium. Fertil. Steril., 56, 98-101. – reference: 2) Kajihara, Y., Goto, K., Kosaka, S., Nakanishi, Y. and Ogawa, K.(1987): In vitro fertilization of bovine follicular oocytes and their development up to hatched blastocysts in vitro. Jpn. J. Anim. Reprod., 33, 173-180 (in Japanese). – reference: 7) Parrish, J. J., Susko-Parrish, J., Winer, M. A. and First, N. L.(1988): Capacitation of bovine sperm by heparin. Biol. Reprod., 38, 1771-1180. – reference: 8) Kim, C. I., Ellington, J. E. and Foote, R. H.(1990): Maturation, fertilization and development of bovine oocytes in vitro using TCM 199 and a simple defined medium with co-culture. Theriogenology, 33, 433-440. – reference: 6) Bavister, B. D. and Yanagimachi, R.(1977): The effects of sperm extract and energy sources on the motility and acrosome reaction of hamster sperm in vitro. Biol. Reprod., 16, 228-237. – reference: 15) Parrish, J. J., Susko-Parrish, J. L., and First, N. L.(1985): Effect of heparin and chondroitin sulfate on the acrosome reaction and fertility of bovine sperm in vitro. Theriogenology, 24, 537-549. – reference: 11) Araki, S., Motoyama, M., Sato, T., Sayama, M., Akahori, A., Tamada, T.(1990): Active sperm separation method used new culture medium GPM for intrafallopian insemination, in vitro fertilization and gamete intrafallopian transfer. Jap. J. Cli. Gyne. Obster., 44, 708-712 (in Japanese). |
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| Title | In Vitro Fertilization of Bovine Oocytes With Serono Gamete Preparation Medium (GPM) |
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