Quantitative evaluation of photoaging mechanisms using analysis of photoacoustic images

In recent years, skin aging caused by ultraviolet light exposure has garnered attention in the fields of beauty and health care because photoaging accelerates skin aging. Elastin is closely related to photoaging. Histopathological diagnosis is the conventional method for evaluating the photoaging sk...

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Published inTransactions of Japanese Society for Medical and Biological Engineering Vol. Annual59; no. Abstract; p. 576
Main Authors Namita, Takeshi, Shiina, Tsuyoshi, Takeshima, Haruna, Yamakawa, Makoto, Kondo, Kengo
Format Journal Article
LanguageJapanese
Published Japanese Society for Medical and Biological Engineering 2021
公益社団法人 日本生体医工学会
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Abstract In recent years, skin aging caused by ultraviolet light exposure has garnered attention in the fields of beauty and health care because photoaging accelerates skin aging. Elastin is closely related to photoaging. Histopathological diagnosis is the conventional method for evaluating the photoaging skin. However, this diagnosis is invasive and has no metabolic function, and its mechanism has not been fully clarified. In this study, photoacoustic imaging was employed for evaluating photoaging mechanisms. This study used acoustic resolution photoacoustic microscopy. An ultrasonically focused transducer attached to the imaging head detected photoacoustic waves. Photoacoustic signal distributions of various photoaging sections were measured. To examine the distinguishability between elastin and collagen, the signal intensity ratio of wavelength 420/450 nm was calculated. The signal intensity ratio of the collagen part is higher than that of elastin part. These results verified the possibility of discriminating elastin from collagen using multi-wavelength analysis of photoacoustic images.
AbstractList In recent years, skin aging caused by ultraviolet light exposure has garnered attention in the fields of beauty and health care because photoaging accelerates skin aging. Elastin is closely related to photoaging. Histopathological diagnosis is the conventional method for evaluating the photoaging skin. However, this diagnosis is invasive and has no metabolic function, and its mechanism has not been fully clarified. In this study, photoacoustic imaging was employed for evaluating photoaging mechanisms. This study used acoustic resolution photoacoustic microscopy. An ultrasonically focused transducer attached to the imaging head detected photoacoustic waves. Photoacoustic signal distributions of various photoaging sections were measured. To examine the distinguishability between elastin and collagen, the signal intensity ratio of wavelength 420/450 nm was calculated. The signal intensity ratio of the collagen part is higher than that of elastin part. These results verified the possibility of discriminating elastin from collagen using multi-wavelength analysis of photoacoustic images. 皮膚の光老化は，紫外線曝露による皮膚の毛細血管の縮退や線維組織の変質などにより起こるといわれている．光老化の評価には摘出した皮膚に対する病理組織学的診断が行われているが，侵襲的で，代謝機能がないといった問題があり，そのメカニズムの解明は十分に行われていない．そこで本研究では，非侵襲でin vivo計測が可能な光音響イメージングにより，より詳細なメカニズムの解明をめざすこととした．超音波の分解能を有するAR-PAMを使用し，可視領域において，光老化の程度が異なるヒト皮膚サンプルの光音響計測を行った．パルス光を脱気水で満たされた水槽を介して試料に照射し，試料からの光音響信号をトランスデューサにより検出した．切片の病理染色像によりコラーゲン部位，エラスチン部位を同定し，それぞれ計測を行った． 各部位における光音響スペクトルを算出し，各波長比を求めた結果，420 nm/450 nmの信号強度比において，エラスチン部位に比べコラーゲン部位の信号強度比が高いことが明らかとなった．そこで，この信号強度比を用いて各切片の空間分布像を作成し，その構成成分を分類した．その結果，光老化の程度の高い切片ではエラスチン成分がコラーゲン成分より多くなり，病理組織学的方法と一致した．これらにより，光音響イメージングにより光老化に関係するコラーゲンとエラスチンを容易に分別でき，光老化のメカニズム解明に有用な情報が得られる可能性を実証した．
In recent years, skin aging caused by ultraviolet light exposure has garnered attention in the fields of beauty and health care because photoaging accelerates skin aging. Elastin is closely related to photoaging. Histopathological diagnosis is the conventional method for evaluating the photoaging skin. However, this diagnosis is invasive and has no metabolic function, and its mechanism has not been fully clarified. In this study, photoacoustic imaging was employed for evaluating photoaging mechanisms. This study used acoustic resolution photoacoustic microscopy. An ultrasonically focused transducer attached to the imaging head detected photoacoustic waves. Photoacoustic signal distributions of various photoaging sections were measured. To examine the distinguishability between elastin and collagen, the signal intensity ratio of wavelength 420/450 nm was calculated. The signal intensity ratio of the collagen part is higher than that of elastin part. These results verified the possibility of discriminating elastin from collagen using multi-wavelength analysis of photoacoustic images.
Author Takeshima, Haruna
Namita, Takeshi
Yamakawa, Makoto
Kondo, Kengo
Shiina, Tsuyoshi
Author_FL 竹嶋 悠菜
椎名 毅
山川 誠
近藤 健悟
浪田 健
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Title Quantitative evaluation of photoaging mechanisms using analysis of photoacoustic images
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