A dual-color Total Internal Reflection (TIR)-based chip for simultaneous detection of two fluorophores

• Published in: 2008 IEEE Sensors pp. 1191 - 1194
• Main Authors: Nam Cao Hoai Le, Dzung Viet Dao, Yokokawa, R., Wells, J., Sugiyama, S.
• Format: Conference Proceeding
• Language: English
• Published: IEEE 01.10.2008
• Subjects: Fiber lasers; Fluorescence; Glass; Lenses; Micromachining; Microoptics; Microscopy; Optical fibers; Optical reflection; Optical refraction

We report a novel, dual-color total internal reflection (TIR)-based chip which can generate two overlapping evanescent fields for simultaneous detection of two fluorophores. The chip was monolithically fabricated using Si bulk micromachining and PDMS casting. Our proposed method integrated all minia...