Anomalies of the anaerobic tricarboxylic acid cycle in Shewanella oneidensis revealed by Tn-seq

• Published in: Molecular microbiology Vol. 86; no. 2; pp. 273 - 283
• Main Authors: Brutinel, Evan D., Gralnick, Jeffrey A.
• Format: Journal Article
• Language: English
• Published: Oxford Blackwell Publishing Ltd 01.10.2012; Blackwell
• Subjects: Anaerobic respiration; Anaerobiosis; Bacterial Proteins - genetics; Bacterial Proteins - metabolism; Bacteriology; Biological and medical sciences; Bioremediation; Biotechnology; Citric Acid Cycle; Fundamental and applied biological sciences. Psychology; Gene expression; Gene Expression Regulation, Bacterial; Genetics; Gram-negative bacteria; High-Throughput Nucleotide Sequencing; Metabolism; Microbiology; Miscellaneous; Molecular biology; Mutagenesis; Mutation; Proteins; Shewanella - genetics; Shewanella - metabolism; Bacteria; Vibrionaceae; Shewanella; Krebs cycle

Summary The availability of increasingly inexpensive sequencing combined with an ever‐expanding molecular biology toolbox has transported classical bacterial genetics into the 21st century. Whole genome genetic fitness analysis using transposon mutagenesis combined with next‐generation high‐throughp...