Increased Serum Levels of Pigment Epithelium-Derived Factor by Excessive Alcohol Consumption-Detection and Identification by a Three-Step Serum Proteome Analysis
Background: The search for biological markers of alcohol abuse is of continual interest in experimental and clinical alcohol research. We previously used gel‐free proteome analysis methods such as the ProteinChip® system and the ClinProt™ system to search for new serum markers for alcoholism and fo...
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| Published in | Alcoholism, clinical and experimental research Vol. 35; no. 2; pp. 211 - 217 |
|---|---|
| Main Authors | , , , , , , , , |
| Format | Journal Article |
| Language | English |
| Published |
Oxford, UK
Blackwell Publishing Ltd
01.02.2011
Wiley |
| Subjects | |
| Online Access | Get full text |
| ISSN | 0145-6008 1530-0277 1530-0277 |
| DOI | 10.1111/j.1530-0277.2010.01336.x |
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| Abstract | Background: The search for biological markers of alcohol abuse is of continual interest in experimental and clinical alcohol research. We previously used gel‐free proteome analysis methods such as the ProteinChip® system and the ClinProt™ system to search for new serum markers for alcoholism and found several novel marker candidates. As serum contains thousands of proteins and peptides that are present in a large dynamic concentration, depletion of the abundant proteins and further fractionation of the remainder is necessary to get into the deep proteome. We recently described a simple and highly reproducible three‐step method for identifying potential disease‐marker candidates among the low‐abundance serum proteins.
Methods: Two serum samples—one on admission and one after 8 weeks of abstinence—were obtained from 8 patients with alcohol dependency. The samples were subjected to a three‐step serum proteome analysis. The steps were the following: first, immunodepletion of the 6 most abundant proteins; second, fractionation using reverse‐phase high‐performance liquid chromatography; and third, separation using one‐dimensional sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS–PAGE). Differences revealed by protein staining were further confirmed by Western blotting and by enzyme‐linked immunosorbent assays (ELISA).
Results: Three‐step serum proteome analysis revealed that the serum levels of 5 proteins, alpha2‐HS glycoprotein, apolipoprotein A‐I, glutathione peroxidase 3, heparin cofactor II, and pigment epithelial‐derived factor (PEDF), were significantly greater on admission than after 8 weeks of abstinence. We focused on PEDF because alterations in its levels in alcoholic subjects are not well known. Western blotting and ELISA confirmed the upregulation of PEDF. Serum PEDF levels were significantly greater in moderate to heavy habitual drinkers (14.2 ± 7.7 μg/ml) than in healthy subjects without a drinking history (5.5 ± 3.0 μg/ml) (p < 0.001). The serum PEDF levels in subjects with nonalcoholic chronic liver diseases were comparable to the PEDF levels in healthy subjects.
Conclusion: Three‐step serum proteome analysis reveals that excessive alcohol drinking increases the PEDF level. |
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| AbstractList | Background: The search for biological markers of alcohol abuse is of continual interest in experimental and clinical alcohol research. We previously used gel‐free proteome analysis methods such as the ProteinChip® system and the ClinProt™ system to search for new serum markers for alcoholism and found several novel marker candidates. As serum contains thousands of proteins and peptides that are present in a large dynamic concentration, depletion of the abundant proteins and further fractionation of the remainder is necessary to get into the deep proteome. We recently described a simple and highly reproducible three‐step method for identifying potential disease‐marker candidates among the low‐abundance serum proteins.
Methods: Two serum samples—one on admission and one after 8 weeks of abstinence—were obtained from 8 patients with alcohol dependency. The samples were subjected to a three‐step serum proteome analysis. The steps were the following: first, immunodepletion of the 6 most abundant proteins; second, fractionation using reverse‐phase high‐performance liquid chromatography; and third, separation using one‐dimensional sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS–PAGE). Differences revealed by protein staining were further confirmed by Western blotting and by enzyme‐linked immunosorbent assays (ELISA).
Results: Three‐step serum proteome analysis revealed that the serum levels of 5 proteins, alpha2‐HS glycoprotein, apolipoprotein A‐I, glutathione peroxidase 3, heparin cofactor II, and pigment epithelial‐derived factor (PEDF), were significantly greater on admission than after 8 weeks of abstinence. We focused on PEDF because alterations in its levels in alcoholic subjects are not well known. Western blotting and ELISA confirmed the upregulation of PEDF. Serum PEDF levels were significantly greater in moderate to heavy habitual drinkers (14.2 ± 7.7 μg/ml) than in healthy subjects without a drinking history (5.5 ± 3.0 μg/ml) (p < 0.001). The serum PEDF levels in subjects with nonalcoholic chronic liver diseases were comparable to the PEDF levels in healthy subjects.
Conclusion: Three‐step serum proteome analysis reveals that excessive alcohol drinking increases the PEDF level. The search for biological markers of alcohol abuse is of continual interest in experimental and clinical alcohol research. We previously used gel-free proteome analysis methods such as the ProteinChip(®) system and the ClinProt™ system to search for new serum markers for alcoholism and found several novel marker candidates. As serum contains thousands of proteins and peptides that are present in a large dynamic concentration, depletion of the abundant proteins and further fractionation of the remainder is necessary to get into the deep proteome. We recently described a simple and highly reproducible three-step method for identifying potential disease-marker candidates among the low-abundance serum proteins.BACKGROUNDThe search for biological markers of alcohol abuse is of continual interest in experimental and clinical alcohol research. We previously used gel-free proteome analysis methods such as the ProteinChip(®) system and the ClinProt™ system to search for new serum markers for alcoholism and found several novel marker candidates. As serum contains thousands of proteins and peptides that are present in a large dynamic concentration, depletion of the abundant proteins and further fractionation of the remainder is necessary to get into the deep proteome. We recently described a simple and highly reproducible three-step method for identifying potential disease-marker candidates among the low-abundance serum proteins.Two serum samples-one on admission and one after 8 weeks of abstinence-were obtained from 8 patients with alcohol dependency. The samples were subjected to a three-step serum proteome analysis. The steps were the following: first, immunodepletion of the 6 most abundant proteins; second, fractionation using reverse-phase high-performance liquid chromatography; and third, separation using one-dimensional sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). Differences revealed by protein staining were further confirmed by Western blotting and by enzyme-linked immunosorbent assays (ELISA).METHODSTwo serum samples-one on admission and one after 8 weeks of abstinence-were obtained from 8 patients with alcohol dependency. The samples were subjected to a three-step serum proteome analysis. The steps were the following: first, immunodepletion of the 6 most abundant proteins; second, fractionation using reverse-phase high-performance liquid chromatography; and third, separation using one-dimensional sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). Differences revealed by protein staining were further confirmed by Western blotting and by enzyme-linked immunosorbent assays (ELISA).Three-step serum proteome analysis revealed that the serum levels of 5 proteins, alpha2-HS glycoprotein, apolipoprotein A-I, glutathione peroxidase 3, heparin cofactor II, and pigment epithelial-derived factor (PEDF), were significantly greater on admission than after 8 weeks of abstinence. We focused on PEDF because alterations in its levels in alcoholic subjects are not well known. Western blotting and ELISA confirmed the upregulation of PEDF. Serum PEDF levels were significantly greater in moderate to heavy habitual drinkers (14.2 ± 7.7 μg/ml) than in healthy subjects without a drinking history (5.5 ± 3.0 μg/ml) (p < 0.001). The serum PEDF levels in subjects with nonalcoholic chronic liver diseases were comparable to the PEDF levels in healthy subjects.RESULTSThree-step serum proteome analysis revealed that the serum levels of 5 proteins, alpha2-HS glycoprotein, apolipoprotein A-I, glutathione peroxidase 3, heparin cofactor II, and pigment epithelial-derived factor (PEDF), were significantly greater on admission than after 8 weeks of abstinence. We focused on PEDF because alterations in its levels in alcoholic subjects are not well known. Western blotting and ELISA confirmed the upregulation of PEDF. Serum PEDF levels were significantly greater in moderate to heavy habitual drinkers (14.2 ± 7.7 μg/ml) than in healthy subjects without a drinking history (5.5 ± 3.0 μg/ml) (p < 0.001). The serum PEDF levels in subjects with nonalcoholic chronic liver diseases were comparable to the PEDF levels in healthy subjects. Three-step serum proteome analysis reveals that excessive alcohol drinking increases the PEDF level.CONCLUSION Three-step serum proteome analysis reveals that excessive alcohol drinking increases the PEDF level. The search for biological markers of alcohol abuse is of continual interest in experimental and clinical alcohol research. We previously used gel-free proteome analysis methods such as the ProteinChip(®) system and the ClinProt™ system to search for new serum markers for alcoholism and found several novel marker candidates. As serum contains thousands of proteins and peptides that are present in a large dynamic concentration, depletion of the abundant proteins and further fractionation of the remainder is necessary to get into the deep proteome. We recently described a simple and highly reproducible three-step method for identifying potential disease-marker candidates among the low-abundance serum proteins. Two serum samples-one on admission and one after 8 weeks of abstinence-were obtained from 8 patients with alcohol dependency. The samples were subjected to a three-step serum proteome analysis. The steps were the following: first, immunodepletion of the 6 most abundant proteins; second, fractionation using reverse-phase high-performance liquid chromatography; and third, separation using one-dimensional sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). Differences revealed by protein staining were further confirmed by Western blotting and by enzyme-linked immunosorbent assays (ELISA). Three-step serum proteome analysis revealed that the serum levels of 5 proteins, alpha2-HS glycoprotein, apolipoprotein A-I, glutathione peroxidase 3, heparin cofactor II, and pigment epithelial-derived factor (PEDF), were significantly greater on admission than after 8 weeks of abstinence. We focused on PEDF because alterations in its levels in alcoholic subjects are not well known. Western blotting and ELISA confirmed the upregulation of PEDF. Serum PEDF levels were significantly greater in moderate to heavy habitual drinkers (14.2 ± 7.7 μg/ml) than in healthy subjects without a drinking history (5.5 ± 3.0 μg/ml) (p < 0.001). The serum PEDF levels in subjects with nonalcoholic chronic liver diseases were comparable to the PEDF levels in healthy subjects. Three-step serum proteome analysis reveals that excessive alcohol drinking increases the PEDF level. |
| Author | Nomura, Fumio Satoh, Mamoru Sogawa, Kazuyuki Maruyama, Katsuya Umemura, Hiroshi Kawashima, Yusuke Yokosuka, Osamu Kodera, Yoshio Takizawa, Hirotaka |
| Author_xml | – sequence: 1 givenname: Kazuyuki surname: Sogawa fullname: Sogawa, Kazuyuki organization: From the Clinical Proteomics Research Center (KS, YK, MS, YK, FN), Chiba University Hospital, Chiba, Japan; Department of Physics (YK), School of Science, Kitasato University, Kanagawa, Japan; Department of Molecular Diagnosis (HU, FN), Graduate School of Medicine, Chiba University, Chiba, Japan; National Hospital Organization Kurihama Alcoholism Center (KM), Kanagawa, Japan; Kashiwado Clinic in Port-Square (HT), Kashiwado Memorial Foundation, Chiba, Japan; and Division of Gastroenterology (OY), Chiba University Hospital, Chiba, Japan – sequence: 2 givenname: Yoshio surname: Kodera fullname: Kodera, Yoshio organization: From the Clinical Proteomics Research Center (KS, YK, MS, YK, FN), Chiba University Hospital, Chiba, Japan; Department of Physics (YK), School of Science, Kitasato University, Kanagawa, Japan; Department of Molecular Diagnosis (HU, FN), Graduate School of Medicine, Chiba University, Chiba, Japan; National Hospital Organization Kurihama Alcoholism Center (KM), Kanagawa, Japan; Kashiwado Clinic in Port-Square (HT), Kashiwado Memorial Foundation, Chiba, Japan; and Division of Gastroenterology (OY), Chiba University Hospital, Chiba, Japan – sequence: 3 givenname: Mamoru surname: Satoh fullname: Satoh, Mamoru organization: From the Clinical Proteomics Research Center (KS, YK, MS, YK, FN), Chiba University Hospital, Chiba, Japan; Department of Physics (YK), School of Science, Kitasato University, Kanagawa, Japan; Department of Molecular Diagnosis (HU, FN), Graduate School of Medicine, Chiba University, Chiba, Japan; National Hospital Organization Kurihama Alcoholism Center (KM), Kanagawa, Japan; Kashiwado Clinic in Port-Square (HT), Kashiwado Memorial Foundation, Chiba, Japan; and Division of Gastroenterology (OY), Chiba University Hospital, Chiba, Japan – sequence: 4 givenname: Yusuke surname: Kawashima fullname: Kawashima, Yusuke organization: From the Clinical Proteomics Research Center (KS, YK, MS, YK, FN), Chiba University Hospital, Chiba, Japan; Department of Physics (YK), School of Science, Kitasato University, Kanagawa, Japan; Department of Molecular Diagnosis (HU, FN), Graduate School of Medicine, Chiba University, Chiba, Japan; National Hospital Organization Kurihama Alcoholism Center (KM), Kanagawa, Japan; Kashiwado Clinic in Port-Square (HT), Kashiwado Memorial Foundation, Chiba, Japan; and Division of Gastroenterology (OY), Chiba University Hospital, Chiba, Japan – sequence: 5 givenname: Hiroshi surname: Umemura fullname: Umemura, Hiroshi organization: From the Clinical Proteomics Research Center (KS, YK, MS, YK, FN), Chiba University Hospital, Chiba, Japan; Department of Physics (YK), School of Science, Kitasato University, Kanagawa, Japan; Department of Molecular Diagnosis (HU, FN), Graduate School of Medicine, Chiba University, Chiba, Japan; National Hospital Organization Kurihama Alcoholism Center (KM), Kanagawa, Japan; Kashiwado Clinic in Port-Square (HT), Kashiwado Memorial Foundation, Chiba, Japan; and Division of Gastroenterology (OY), Chiba University Hospital, Chiba, Japan – sequence: 6 givenname: Katsuya surname: Maruyama fullname: Maruyama, Katsuya organization: From the Clinical Proteomics Research Center (KS, YK, MS, YK, FN), Chiba University Hospital, Chiba, Japan; Department of Physics (YK), School of Science, Kitasato University, Kanagawa, Japan; Department of Molecular Diagnosis (HU, FN), Graduate School of Medicine, Chiba University, Chiba, Japan; National Hospital Organization Kurihama Alcoholism Center (KM), Kanagawa, Japan; Kashiwado Clinic in Port-Square (HT), Kashiwado Memorial Foundation, Chiba, Japan; and Division of Gastroenterology (OY), Chiba University Hospital, Chiba, Japan – sequence: 7 givenname: Hirotaka surname: Takizawa fullname: Takizawa, Hirotaka organization: From the Clinical Proteomics Research Center (KS, YK, MS, YK, FN), Chiba University Hospital, Chiba, Japan; Department of Physics (YK), School of Science, Kitasato University, Kanagawa, Japan; Department of Molecular Diagnosis (HU, FN), Graduate School of Medicine, Chiba University, Chiba, Japan; National Hospital Organization Kurihama Alcoholism Center (KM), Kanagawa, Japan; Kashiwado Clinic in Port-Square (HT), Kashiwado Memorial Foundation, Chiba, Japan; and Division of Gastroenterology (OY), Chiba University Hospital, Chiba, Japan – sequence: 8 givenname: Osamu surname: Yokosuka fullname: Yokosuka, Osamu organization: From the Clinical Proteomics Research Center (KS, YK, MS, YK, FN), Chiba University Hospital, Chiba, Japan; Department of Physics (YK), School of Science, Kitasato University, Kanagawa, Japan; Department of Molecular Diagnosis (HU, FN), Graduate School of Medicine, Chiba University, Chiba, Japan; National Hospital Organization Kurihama Alcoholism Center (KM), Kanagawa, Japan; Kashiwado Clinic in Port-Square (HT), Kashiwado Memorial Foundation, Chiba, Japan; and Division of Gastroenterology (OY), Chiba University Hospital, Chiba, Japan – sequence: 9 givenname: Fumio surname: Nomura fullname: Nomura, Fumio organization: From the Clinical Proteomics Research Center (KS, YK, MS, YK, FN), Chiba University Hospital, Chiba, Japan; Department of Physics (YK), School of Science, Kitasato University, Kanagawa, Japan; Department of Molecular Diagnosis (HU, FN), Graduate School of Medicine, Chiba University, Chiba, Japan; National Hospital Organization Kurihama Alcoholism Center (KM), Kanagawa, Japan; Kashiwado Clinic in Port-Square (HT), Kashiwado Memorial Foundation, Chiba, Japan; and Division of Gastroenterology (OY), Chiba University Hospital, Chiba, Japan |
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| Keywords | Consumption Biological fluid Alcoholism Proteome Identification Alcoholic beverage Pigment Epithelial-Derived Factor Proteomics Pigments Serum Diagnosis Pigment epithelium-derived factor Detection |
| Language | English |
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| References | Yamagishi S, Matsui T, Nakamura K (2009) Atheroprotective properties of pigment epithelium-derived factor (PEDF) in cardiometabolic disorders. Curr Pharm Des 15:1027-1033. Umemura H, Nezu M, Kodera Y, Satoh M, Kimura A, Tomonaga T, Nomura F (2009) Effects of the time intervals between venipuncture and serum preparation for serum peptidome analysis by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. Clin Chim Acta 406:179-180. Nanchahal K, Ashton WD, Wood DA (2000) Alcohol consumption, metabolic cardiovascular risk factors and hypertension in women. Int J Epidemiol 29:57-64. Petersen SV, Valnickova Z, Enghild JJ (2003) Pigment-epithelium-derived factor (PEDF) occurs at a physiologically relevant concentration in human blood: purification and characterization. Biochem J 15:199-206. Conigrave KM, Degenhardt LJ, Whitfield JB, Saunders JB, Helander A, Tabakoff B (2002) CDT, GGT, and AST as markers of alcohol use: the WHO/ISBRA collaborative project. Alcohol Clin Exp Res 26:332-339. Kawashima Y, Fukuno T, Satoh M, Takahashi H, Matsui T, Maeda T, Kodera Y (2009) A simple and highly reproducible method for discovering potential disease markers in low abundance serum proteins. J Electrophor 53:13-18. Tombran-Tink J, Barnstable CJ (2003) PEDF: a multifaceted neurotrophic factor. Nat Rev Neurosci 4:628-636. Anderson NL, Anderson NG (2002) The human plasma proteome: history, character, and diagnostic prospects. Mol Cell Proteomics 1:845-867. Hannuksela ML, Liisanantti MK, Nissinen AE, Savolainen MJ (2007) Biochemical markers of alcoholism. Clin Chem Lab Med 45:953-961. Tombran-Tink J, Chader CG, Johnson LV (1991) PEDF: pigment epithelium-derived factor with potent neuronal differentiative activity. Exp Eye Res 53:411-414. Ogata N, Matsuoka M, Matsuyama K, Shima C, Tajika A, Nishiyama T, Wada M, Jo N, Higuchi A, Minamino K, Matsunaga H, Takeda T, Matsumura M (2007) Plasma concentration of pigment epithelium-derived factor in patients with diabetic retinopathy. J Clin Endocrinol Metab 92:1176-1179. Rychli K, Huber K, Wojta J (2009) Pigment epithelium-derived factor (PEDF) as a therapeutic target in cardiovascular disease. Expert Opin Ther Targets 13:1295-1302. Alling C, Chick JD, Anton R, Mayfield RD, Salaspuro M, Helander A, Harris RA (2005) Revealing alcohol abuse: to ask or to test? Alcohol Clin Exp Res 29:1257-1263. Hattori N, Oda S, Sadahiro T, Nakamura M, Abe R, Shinozaki K, Nomura F, Tomonaga T, Matsushita K, Kodera Y, Sogawa K, Satoh M, Hirasawa H (2009) YKL-40 identified by proteomic analysis as a biomarker of sepsis. Shock 32:393-400. Niemela O (2007) Biomarkers in alcoholism. Clin Chim Acta 377:39-49. Nomura F, Tomonaga T, Sogawa K, Ohashi T, Nezu M, Sunaga M, Kondo N, Iyo M, Shimada H, Ochiai T (2004) Identification of novel and downregulated biomarkers for alcoholism by surface enhanced laser desorption/ionization-mass spectrometry. Proteomics 4:1187-1194. Uehara H, Miyamoto M, Kato K, Ebihara Y, Kaneko H, Hashimoto H, Murakami Y, Hase R, Takahashi R, Mega S, Shichinohe T, Kawarada Y, Itoh T, Okushiba S, Kondo S, Katoh H (2004) Expression of pigment epithelium-derived factor decreases liver metastasis and correlates with favorable prognosis for patients with ductal pancreatic adenocarcinoma. Cancer Res 64:3533-3537. Peng FC, Tang SH, Huang MC, Chen CC, Kuo TL, Yin SJ (2005) Oxidative status in patients with alcohol dependence: a clinical study in Taiwan. J Toxicol Environ Health A 17-18:1497-1509. Yamagishi S, Adachi H, Abe A, Yashiro T, Enomoto M, Furuki K, Hino A, Jinnouchi Y, Takenaka K, Matsui T, Nakamura K, Imaizumi T (2006) Elevated serum levels of pigment epithelium-derived factor in the metabolic syndrome. J Clin Endocrinol Metab 91:2447-2450. Leung DW, Cachianes G, Kuang WJ, Goeddel DV, Ferrara N (1989) Vascular endothelial growth factor is a secreted angiogenic mitogen. Science 246:1306-1309. Sogawa K, Satoh M, Kodera Y, Tomonaga T, Iyo M, Nomura F (2009) A search for novel markers of alcohol abuse using magnetic beads and MALDI-TOF/TOF mass spectrometry. Proteomics Clin Appl 3:821-828. Wu D, Tomonaga T, Sogawa K, Satoh M, Sunaga M, Nezu M, Oh-Ishi M, Kodera Y, Maeda T, Ochiai T, Nomura F (2007) Detection of biomarkers for alcoholism by two-dimensional differential gel electrophoresis. Alcohol Clin Exp Res 31:67S-71S. Matsumoto K, Ishikawa H, Nishimura D, Hamasaki K, Nakao K, Eguchi K (2004) Antiangiogenic property of pigment epithelium-derived factor in hepatocellular carcinoma. Hepatology 40:252-259. Robinson SF, Quarfordt SH (1981) The effect of ethanol on lipoprotein metabolism. Alcohol Clin Exp Res 5:101-109. Yamagishi S, Matsui T (2010) Anti-atherothrombogenic properties of PEDF. Curr Mol Med 10:284-291. Sogawa K, Itoga S, Tomonaga T, Nomura F (2007) Diagnostic values of surface-enhanced laser desorption/ionization technology for screening of habitual drinkers. Alcohol Clin Exp Res 31:22S-26S. Andersson TR, Bell H (1988) Plasma heparin cofactor II in alcohol liver disease. J Hepatol 7:79-84. Renaud S, Lanzmann-Petithory D, Gueguen R, Conard P (2004) Alcohol and mortality from all causes. Biol Res 37:183-187. Nomura F, Tomonaga T, Sogawa K, Wu D, Ohashi T (2007) Application of proteomic technologies to discover and identify biomarkers for excessive alcohol consumption: a review. J Chromatogr B Analyt Technol Biomed Life Sci 855:35-41. Tirumalai RS, Chan KC, Prieto DA, Issaq HJ, Conrads TP, Veenstra TD (2003) Characterization of the low molecular weight human serum proteome. Mol Cell Proteomics 2:1096-1103. Kaku Y, Hasumura Y, Takeuchi J (1982) Clinical detection of the hepatic lesion of pericentral sclerosis in chronic alcoholics. Gut 23:215-220. 2010; 10 2004; 64 2006; 91 2000; 29 2004; 40 1991; 53 1981; 5 2002; 1 2004; 4 2003; 15 2007; 92 2007; 31 2005; 29 2005; 17–18 1982; 23 2002; 26 2007; 377 2009; 13 2007; 855 2009; 32 2009; 53 1989; 246 2004; 37 1988; 7 2003; 2 2003; 4 2009; 3 2009; 406 2007; 45 2009; 15 |
| References_xml | – reference: Alling C, Chick JD, Anton R, Mayfield RD, Salaspuro M, Helander A, Harris RA (2005) Revealing alcohol abuse: to ask or to test? Alcohol Clin Exp Res 29:1257-1263. – reference: Sogawa K, Satoh M, Kodera Y, Tomonaga T, Iyo M, Nomura F (2009) A search for novel markers of alcohol abuse using magnetic beads and MALDI-TOF/TOF mass spectrometry. Proteomics Clin Appl 3:821-828. – reference: Hattori N, Oda S, Sadahiro T, Nakamura M, Abe R, Shinozaki K, Nomura F, Tomonaga T, Matsushita K, Kodera Y, Sogawa K, Satoh M, Hirasawa H (2009) YKL-40 identified by proteomic analysis as a biomarker of sepsis. Shock 32:393-400. – reference: Sogawa K, Itoga S, Tomonaga T, Nomura F (2007) Diagnostic values of surface-enhanced laser desorption/ionization technology for screening of habitual drinkers. Alcohol Clin Exp Res 31:22S-26S. – reference: Tirumalai RS, Chan KC, Prieto DA, Issaq HJ, Conrads TP, Veenstra TD (2003) Characterization of the low molecular weight human serum proteome. Mol Cell Proteomics 2:1096-1103. – reference: Rychli K, Huber K, Wojta J (2009) Pigment epithelium-derived factor (PEDF) as a therapeutic target in cardiovascular disease. Expert Opin Ther Targets 13:1295-1302. – reference: Leung DW, Cachianes G, Kuang WJ, Goeddel DV, Ferrara N (1989) Vascular endothelial growth factor is a secreted angiogenic mitogen. Science 246:1306-1309. – reference: Tombran-Tink J, Barnstable CJ (2003) PEDF: a multifaceted neurotrophic factor. Nat Rev Neurosci 4:628-636. – reference: Yamagishi S, Adachi H, Abe A, Yashiro T, Enomoto M, Furuki K, Hino A, Jinnouchi Y, Takenaka K, Matsui T, Nakamura K, Imaizumi T (2006) Elevated serum levels of pigment epithelium-derived factor in the metabolic syndrome. J Clin Endocrinol Metab 91:2447-2450. – reference: Andersson TR, Bell H (1988) Plasma heparin cofactor II in alcohol liver disease. J Hepatol 7:79-84. – reference: Kaku Y, Hasumura Y, Takeuchi J (1982) Clinical detection of the hepatic lesion of pericentral sclerosis in chronic alcoholics. Gut 23:215-220. – reference: Robinson SF, Quarfordt SH (1981) The effect of ethanol on lipoprotein metabolism. Alcohol Clin Exp Res 5:101-109. – reference: Conigrave KM, Degenhardt LJ, Whitfield JB, Saunders JB, Helander A, Tabakoff B (2002) CDT, GGT, and AST as markers of alcohol use: the WHO/ISBRA collaborative project. Alcohol Clin Exp Res 26:332-339. – reference: Yamagishi S, Matsui T, Nakamura K (2009) Atheroprotective properties of pigment epithelium-derived factor (PEDF) in cardiometabolic disorders. Curr Pharm Des 15:1027-1033. – reference: Matsumoto K, Ishikawa H, Nishimura D, Hamasaki K, Nakao K, Eguchi K (2004) Antiangiogenic property of pigment epithelium-derived factor in hepatocellular carcinoma. Hepatology 40:252-259. – reference: Peng FC, Tang SH, Huang MC, Chen CC, Kuo TL, Yin SJ (2005) Oxidative status in patients with alcohol dependence: a clinical study in Taiwan. J Toxicol Environ Health A 17-18:1497-1509. – reference: Hannuksela ML, Liisanantti MK, Nissinen AE, Savolainen MJ (2007) Biochemical markers of alcoholism. Clin Chem Lab Med 45:953-961. – reference: Umemura H, Nezu M, Kodera Y, Satoh M, Kimura A, Tomonaga T, Nomura F (2009) Effects of the time intervals between venipuncture and serum preparation for serum peptidome analysis by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. Clin Chim Acta 406:179-180. – reference: Nomura F, Tomonaga T, Sogawa K, Ohashi T, Nezu M, Sunaga M, Kondo N, Iyo M, Shimada H, Ochiai T (2004) Identification of novel and downregulated biomarkers for alcoholism by surface enhanced laser desorption/ionization-mass spectrometry. Proteomics 4:1187-1194. – reference: Ogata N, Matsuoka M, Matsuyama K, Shima C, Tajika A, Nishiyama T, Wada M, Jo N, Higuchi A, Minamino K, Matsunaga H, Takeda T, Matsumura M (2007) Plasma concentration of pigment epithelium-derived factor in patients with diabetic retinopathy. J Clin Endocrinol Metab 92:1176-1179. – reference: Uehara H, Miyamoto M, Kato K, Ebihara Y, Kaneko H, Hashimoto H, Murakami Y, Hase R, Takahashi R, Mega S, Shichinohe T, Kawarada Y, Itoh T, Okushiba S, Kondo S, Katoh H (2004) Expression of pigment epithelium-derived factor decreases liver metastasis and correlates with favorable prognosis for patients with ductal pancreatic adenocarcinoma. Cancer Res 64:3533-3537. – reference: Niemela O (2007) Biomarkers in alcoholism. Clin Chim Acta 377:39-49. – reference: Tombran-Tink J, Chader CG, Johnson LV (1991) PEDF: pigment epithelium-derived factor with potent neuronal differentiative activity. Exp Eye Res 53:411-414. – reference: Kawashima Y, Fukuno T, Satoh M, Takahashi H, Matsui T, Maeda T, Kodera Y (2009) A simple and highly reproducible method for discovering potential disease markers in low abundance serum proteins. J Electrophor 53:13-18. – reference: Nomura F, Tomonaga T, Sogawa K, Wu D, Ohashi T (2007) Application of proteomic technologies to discover and identify biomarkers for excessive alcohol consumption: a review. J Chromatogr B Analyt Technol Biomed Life Sci 855:35-41. – reference: Anderson NL, Anderson NG (2002) The human plasma proteome: history, character, and diagnostic prospects. Mol Cell Proteomics 1:845-867. – reference: Petersen SV, Valnickova Z, Enghild JJ (2003) Pigment-epithelium-derived factor (PEDF) occurs at a physiologically relevant concentration in human blood: purification and characterization. Biochem J 15:199-206. – reference: Wu D, Tomonaga T, Sogawa K, Satoh M, Sunaga M, Nezu M, Oh-Ishi M, Kodera Y, Maeda T, Ochiai T, Nomura F (2007) Detection of biomarkers for alcoholism by two-dimensional differential gel electrophoresis. Alcohol Clin Exp Res 31:67S-71S. – reference: Nanchahal K, Ashton WD, Wood DA (2000) Alcohol consumption, metabolic cardiovascular risk factors and hypertension in women. Int J Epidemiol 29:57-64. – reference: Yamagishi S, Matsui T (2010) Anti-atherothrombogenic properties of PEDF. Curr Mol Med 10:284-291. – reference: Renaud S, Lanzmann-Petithory D, Gueguen R, Conard P (2004) Alcohol and mortality from all causes. Biol Res 37:183-187. – volume: 26 start-page: 332 year: 2002 end-page: 339 article-title: CDT, GGT, and AST as markers of alcohol use: the WHO/ISBRA collaborative project publication-title: Alcohol Clin Exp Res – volume: 10 start-page: 284 year: 2010 end-page: 291 article-title: Anti‐atherothrombogenic properties of PEDF publication-title: Curr Mol Med – volume: 29 start-page: 1257 year: 2005 end-page: 1263 article-title: Revealing alcohol abuse: to ask or to test? publication-title: Alcohol Clin Exp Res – volume: 2 start-page: 1096 year: 2003 end-page: 1103 article-title: Characterization of the low molecular weight human serum proteome publication-title: Mol Cell Proteomics – volume: 1 start-page: 845 year: 2002 end-page: 867 article-title: The human plasma proteome: history, character, and diagnostic prospects publication-title: Mol Cell Proteomics – volume: 3 start-page: 821 year: 2009 end-page: 828 article-title: A search for novel markers of alcohol abuse using magnetic beads and MALDI‐TOF/TOF mass spectrometry publication-title: Proteomics Clin Appl – volume: 45 start-page: 953 year: 2007 end-page: 961 article-title: Biochemical markers of alcoholism publication-title: Clin Chem Lab Med – volume: 377 start-page: 39 year: 2007 end-page: 49 article-title: Biomarkers in alcoholism publication-title: Clin Chim Acta – volume: 92 start-page: 1176 year: 2007 end-page: 1179 article-title: Plasma concentration of pigment epithelium‐derived factor in patients with diabetic retinopathy publication-title: J Clin Endocrinol Metab – volume: 15 start-page: 1027 year: 2009 end-page: 1033 article-title: Atheroprotective properties of pigment epithelium‐derived factor (PEDF) in cardiometabolic disorders publication-title: Curr Pharm Des – volume: 5 start-page: 101 year: 1981 end-page: 109 article-title: The effect of ethanol on lipoprotein metabolism publication-title: Alcohol Clin Exp Res – volume: 15 start-page: 199 year: 2003 end-page: 206 article-title: Pigment‐epithelium‐derived factor (PEDF) occurs at a physiologically relevant concentration in human blood: purification and characterization publication-title: Biochem J – volume: 406 start-page: 179 year: 2009 end-page: 180 article-title: Effects of the time intervals between venipuncture and serum preparation for serum peptidome analysis by matrix‐assisted laser desorption/ionization time‐of‐flight mass spectrometry publication-title: Clin Chim Acta – volume: 32 start-page: 393 year: 2009 end-page: 400 article-title: YKL‐40 identified by proteomic analysis as a biomarker of sepsis publication-title: Shock – volume: 29 start-page: 57 year: 2000 end-page: 64 article-title: Alcohol consumption, metabolic cardiovascular risk factors and hypertension in women publication-title: Int J Epidemiol – volume: 855 start-page: 35 year: 2007 end-page: 41 article-title: Application of proteomic technologies to discover and identify biomarkers for excessive alcohol consumption: a review publication-title: J Chromatogr B Analyt Technol Biomed Life Sci – volume: 23 start-page: 215 year: 1982 end-page: 220 article-title: Clinical detection of the hepatic lesion of pericentral sclerosis in chronic alcoholics publication-title: Gut – volume: 31 start-page: 22S year: 2007 end-page: 26S article-title: Diagnostic values of surface‐enhanced laser desorption/ionization technology for screening of habitual drinkers publication-title: Alcohol Clin Exp Res – volume: 4 start-page: 1187 year: 2004 end-page: 1194 article-title: Identification of novel and downregulated biomarkers for alcoholism by surface enhanced laser desorption/ionization‐mass spectrometry publication-title: Proteomics – volume: 4 start-page: 628 year: 2003 end-page: 636 article-title: PEDF: a multifaceted neurotrophic factor publication-title: Nat Rev Neurosci – volume: 53 start-page: 411 year: 1991 end-page: 414 article-title: PEDF: pigment epithelium‐derived factor with potent neuronal differentiative activity publication-title: Exp Eye Res – volume: 31 start-page: 67S year: 2007 end-page: 71S article-title: Detection of biomarkers for alcoholism by two‐dimensional differential gel electrophoresis publication-title: Alcohol Clin Exp Res – volume: 246 start-page: 1306 year: 1989 end-page: 1309 article-title: Vascular endothelial growth factor is a secreted angiogenic mitogen publication-title: Science – volume: 17–18 start-page: 1497 year: 2005 end-page: 1509 article-title: Oxidative status in patients with alcohol dependence: a clinical study in Taiwan publication-title: J Toxicol Environ Health A – volume: 40 start-page: 252 year: 2004 end-page: 259 article-title: Antiangiogenic property of pigment epithelium‐derived factor in hepatocellular carcinoma publication-title: Hepatology – volume: 37 start-page: 183 year: 2004 end-page: 187 article-title: Alcohol and mortality from all causes publication-title: Biol Res – volume: 64 start-page: 3533 year: 2004 end-page: 3537 article-title: Expression of pigment epithelium‐derived factor decreases liver metastasis and correlates with favorable prognosis for patients with ductal pancreatic adenocarcinoma publication-title: Cancer Res – volume: 53 start-page: 13 year: 2009 end-page: 18 article-title: A simple and highly reproducible method for discovering potential disease markers in low abundance serum proteins publication-title: J Electrophor – volume: 13 start-page: 1295 year: 2009 end-page: 1302 article-title: Pigment epithelium‐derived factor (PEDF) as a therapeutic target in cardiovascular disease publication-title: Expert Opin Ther Targets – volume: 91 start-page: 2447 year: 2006 end-page: 2450 article-title: Elevated serum levels of pigment epithelium‐derived factor in the metabolic syndrome publication-title: J Clin Endocrinol Metab – volume: 7 start-page: 79 year: 1988 end-page: 84 article-title: Plasma heparin cofactor II in alcohol liver disease publication-title: J Hepatol |
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| Snippet | Background: The search for biological markers of alcohol abuse is of continual interest in experimental and clinical alcohol research. We previously used... The search for biological markers of alcohol abuse is of continual interest in experimental and clinical alcohol research. We previously used gel-free proteome... |
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| SubjectTerms | Addictive behaviors Adult and adolescent clinical studies Alcohol Drinking - blood Alcoholism Alcoholism - blood Alcoholism and acute alcohol poisoning Biological and medical sciences Biomarkers - blood Blotting, Western Electrophoresis, Polyacrylamide Gel Enzyme-Linked Immunosorbent Assay Eye Proteins - blood Female Humans Male Medical sciences Nerve Growth Factors - blood Pigment Epithelial-Derived Factor Proteome - analysis Proteomics Psychology. Psychoanalysis. Psychiatry Psychopathology. Psychiatry Serpins - blood Serum Toxicology |
| Title | Increased Serum Levels of Pigment Epithelium-Derived Factor by Excessive Alcohol Consumption-Detection and Identification by a Three-Step Serum Proteome Analysis |
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