Development of three vitrification-based cryopreservation procedures for shoot tips of China's potato

• Published in: Cryo-Letters Vol. 34; no. 4; p. 369
• Main Authors: Wang, B, Wang, R R, Li, J W, Ma, Y L, Sheng, W M, Li, M F, Wang, Q C
• Format: Journal Article
• Language: English
• Published: England 01.07.2013
• Subjects: Cryopreservation - methods; Cryoprotective Agents - chemistry; Cryoprotective Agents - metabolism; Plant Shoots - drug effects; Plant Shoots - growth & development; Plant Shoots - physiology; Solanum tuberosum - drug effects; Solanum tuberosum - growth & development; Solanum tuberosum - physiology; Vitrification
• ISSN: 0143-2044

China is now the largest potato producing country worldwide. The establishment of efficient conservation techniques for potato germplasm is a prerequisite for breeding of elite cultivars. Potato viral diseases have been a great threat for sustainable potato production in China. Use of virus-free seed tubers is an effective and practical means to control viral diseases. In the present study, three vitrification-based cryopreservation techniques, i.e. droplet-vitrification, encapsulation-vitrification and vitrification were successfully developed for the first time for China's potato. Cultivar 'Zihuabai' was used to optimize key parameters involved in the three vitrification-based procedures. With the optimized parameters, shoot regrowth percentages of 71%, 76% and 43% were obtained for droplet-vitrification, encapsulation-vitrification and vitrification, respectively. The three protocols developed were further tested with eight China's major cultivars, with average shoot regrowth of 61%, 38% and 28% for droplet-vitrification, encapsulation-vitrification and vitrification, respectively. Successful development of the three cryopreservation procedures using a single cultivar will facilitate a number of comparative studies such as cryo-injury, regrowth pattern, genetic stability and efficiency of virus elimination. Testing these three cryogenic procedures for potato major cultivars representing a wide range of genetic background, will help the establishment of potato cryobanking in China and the production of virus-free plants.