A proteomic analysis of organelles from Arabidopsis thaliana
We introduce the use of Arabidopsis thaliana callus culture as a system for proteomic analysis of plant organelles using liquid‐grown callus. This callus is relatively homogeneous, reproducible and cytoplasmically rich, and provides organelles in sufficient quantities for proteomic studies. A databa...
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Published in | Electrophoresis Vol. 21; no. 16; pp. 3488 - 3499 |
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Main Authors | , , , , |
Format | Journal Article |
Language | English |
Published |
Hoboken
Wiley Subscription Services, Inc., A Wiley Company
01.10.2000
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Subjects | |
Online Access | Get full text |
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Summary: | We introduce the use of Arabidopsis thaliana callus culture as a system for proteomic analysis of plant organelles using liquid‐grown callus. This callus is relatively homogeneous, reproducible and cytoplasmically rich, and provides organelles in sufficient quantities for proteomic studies. A database was generated of mitochondrial, endoplasmic reticulum (ER), Golgi/prevacuolar compartment and plasma membrane (PM) markers using two‐dimensional sodium dodecyl sulphate‐polyacrylamide gel electrophoresis (2‐D SDS‐PAGE) and peptide sequencing or mass spectrometric methods. The major callus membrane‐associated proteins were characterised as being integral or peripheral by Triton X‐114 phase partitioning. The database was used to define specific proteins at the Arabidopsis callus plasma membrane. This database of organelle proteins provides the basis for future characterisation of the expression and localisation of novel plant proteins. |
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Bibliography: | istex:D2D980653663CAE8AACCFAB97412DD43753EF87D ArticleID:ELPS3488 ark:/67375/WNG-RZ369V1D-J ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 0173-0835 1522-2683 |
DOI: | 10.1002/1522-2683(20001001)21:16<3488::AID-ELPS3488>3.0.CO;2-3 |