Cloning and expression of xylanase 10 from Cryptovalsa mangrovei (BCC7197) in Pichia pastoris

• Published in: DNA sequence Vol. 16; no. 5; pp. 372 - 378
• Main Authors: Boonyapakron, Katewadee, Pootanakit, Kusol, Chantasingh, Duriya, Kirtikara, Kanyawim, Eurwilaichitr, Lily
• Format: Journal Article
• Language: English
• Published: England Informa UK Ltd 01.10.2005
• Subjects: Amino Acid Sequence; Ascomycota - enzymology; Cloning, Molecular; DNA Primers; Endo-1,4-beta Xylanases - biosynthesis; Endo-1,4-beta Xylanases - genetics; Endo-1,4-beta Xylanases - isolation & purification; Models, Molecular; Molecular Sequence Data; Pichia - enzymology; Pichia - genetics; Pichia pastoris; Sequence Homology, Amino Acid

Xylanases are one of the industrially valuable enzymes. Using RT-PCR and 5′- and 3′-RACE procedures, we have cloned a full-length xylanase encoding gene from a filamentous fungus, Cryptovalsa mangrovei (BCC7197) from Phuket, Thailand. The results showed that BCC7197 xylanase cDNA has an open reading...