Advancements and challenges in protein purification techniques for denitrifying enzymes: A path to effective nitrogen removal and reduced N2O emissions

• Published in: Critical reviews in environmental science and technology Vol. 54; no. 21; pp. 1546 - 1568
• Main Authors: Liu, Lingxiu, Li, Anhang, Shi, Changjie, Hrynsphan, Dzmitry, Tatsiana, Savitskaya, Wang, Zeyu, Chen, Jun
• Format: Journal Article
• Language: English
• Published: Boca Raton Taylor & Francis 01.11.2024; Taylor & Francis Ltd
• Subjects: active sites; biochemical pathways; Biological activity; Biological effects; chromatographic purification; chromatography; Crude product extraction; Crystallography; Denitrification; denitrifying enzymes; Electron microscopy; Emissions; environmental science; Enzymes; Homogeneity; mechanism of action; nitrogen; Nitrogen removal; Nitrous oxide; NMR; Nuclear magnetic resonance; nuclear magnetic resonance spectroscopy; Peng Gao and Scott Bradford; protein expression; Protein folding; Protein purification; protein purification technology; Protein structure; protein synthesis; Proteins; Purification; technology; X-ray crystallography; X-ray diffraction

The biological denitrification process is crucial for removing nitrogen and reducing nitrous oxide (N 2 O) emissions. To investigate the mechanism of action of key enzymes in the denitrification process, it is necessary to analyze the protein structure, active site, and conformational changes of den...