Monitoring the N-glycosylation of plant glycoproteins by fluorophore-assisted carbohydrate electrophoresis

• Published in: Electrophoresis Vol. 21; no. 12; pp. 2550 - 2556
• Main Authors: Bardor, Muriel, Cabanes-Macheteau, Marion, Faye, Loïc, Lerouge, Patrice
• Format: Journal Article
• Language: English
• Published: Hoboken Wiley Subscription Services, Inc., A Wiley Company 01.07.2000
• Subjects: 6-trisulfonic acid labeled plant N-glycans; 8-Aminonaphthalene-1; 8‐Aminonaphthalene‐1,3,6‐trisulfonic acid labeled plant N‐glycans; Carbohydrate Sequence; Electrophoresis, Polyacrylamide Gel - methods; Fluorescent Dyes; Fluorophore-assisted carbohydrate electrophoresis; Glycoproteins - analysis; Glycosylation; Mannose; Molecular Sequence Data; Naphthalenes; Plant glycoproteins; Plant Proteins - analysis; Polysaccharides - analysis

We have evaluated the efficiency of a fast, simple and efficient method, fluorophore‐assisted carbohydrate electrophoresis (FACE), for the characterization of plant N‐linked glycans. After their enzymatic release from plant glycoproteins, N‐glycans were reductively aminated to the charged fluorophor...