UPLC-Q-Orbitrap-MS2 analysis of Moringa oleifera leaf extract and its antioxidant, antibacterial and anti-inflammatory activities

• Published in: Natural product research Vol. 34; no. 14; pp. 2090 - 2094
• Main Authors: Yan, Guo, Liping, Sun, Yongliang, Zhuang
• Format: Journal Article
• Language: English
• Published: Abingdon Taylor & Francis 17.07.2020; Taylor & Francis Ltd
• Subjects: Acetone; Acids; anti-inflammatory; anti-inflammatory activity; antibacterial; antioxidant; Antioxidants; Caffeoylquinic acid; chlorogenic acid; dose response; E coli; Escherichia coli; Gallic acid; Inflammation; leaf extracts; Leaves; Lipopolysaccharides; Mass spectrometry; Mass spectroscopy; Moringa oleifera; Moringa oleifera leaf; mRNA; Nitric oxide; Nitric-oxide synthase; Phenolic compounds; Phenols; phytochemicals; Plant extracts; Scavenging
• ISSN: 1478-6419; 1478-6427; 1478-6427
• DOI: 10.1080/14786419.2019.1573237

Moringa oleifera leaf acetone extract (MLE) was prepared. Phytochemicals of MLE and their antioxidant, antibacterial, and anti-inflammatory activities were evaluated. Results showed that MLE contained total phenolic content of 20.16 mg gallic acid equivalents/g dry weight. A total of 39 compounds were identified by mass spectrometry. The contents of acetyl-glucomoringin, caffeoylquinic acid, feruloylquinic acid, and coumarylquinic acid were high. MLE had high DPPH· and ABTS *+ scavenging activities and reducing powder. In addition, MLE could effectively inhibit S. aureus and B. subtilis, but little effect on E. coli was found. The anti-inflammatory effect of MLE was evaluated using a lipopolysaccharide (LPS) -induced RAW 264.7 cell model. MLE significantly inhibited nitric oxide (NO) production and inducible NO synthase (iNOS) mRNA levels in LPS-induced RAW 264.7 cells. The inhibitory activity increased in a dose-dependent manner. The bioactivities of MLE were related to its phenolic content and phenolic profiles.