Vignetting and photo-bleaching correction in automated fluorescence microscopy from an array of overlapping images

• Published in: 2013 IEEE 10th International Symposium on Biomedical Imaging pp. 464 - 467
• Main Authors: Piccinini, Filippo, Bevilacqua, Alessandro, Smith, Kevin, Horvath, Peter
• Format: Conference Proceeding
• Language: English
• Published: IEEE 01.04.2013
• Subjects: Additives; Arrays; automated microscopy; Calibration; illumination correction; image processing; Lighting; Microscopy; mosaicing; Optical microscopy; registration

We propose a novel acquisition scheme and non-parametric multi-image based method for correcting illumination in fluorescence images. Our approach measures changes in intensity of the subject by moving the microscope stage at regularly spaced intervals, and exploits this information to learn the cor...