Cylindrical pillars in silicon PCR chip enhance the performance of DNA amplification

• Published in: The 13th International Conference on Solid-State Sensors, Actuators and Microsystems, 2005. Digest of Technical Papers. TRANSDUCERS '05 Vol. 2; pp. 1604 - 1607 Vol. 2
• Main Authors: Se Ho Park, Moon Yun Jung, Tae Hwan Yoon, Hyeon-Bong Pyo
• Format: Conference Proceeding
• Language: English
• Published: IEEE 2005
• Subjects: Bioinformatics; Cancer; DNA; Flexible printed circuits; Genomics; Heat transfer; Reproducibility of results; Sequences; Silicon; Temperature

This paper presents a new design of a silicon PCR chamber with cylindrical pillars on the bottom which showed good performance in heat transfer and DNA amplification efficiency comparable to thermocycler. We successfully amplified the full DNA sequence of N-acetyl transferase 2 (NAT2) gene, cancer r...