Rapid Method for Analysis of Aspirin-Butalbital in Serum Utilizing a Monolithic C18 Column
A fast and sensitive high performance liquid chromatography (HPLC) assay was developed for the simultaneous determination of aspirin-butalbital in human serum. Serum samples were treated with a solid phase extraction procedure. The analytes were separated using a mobile phase of 90:10 (v/v) 0.1 M aq...
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Published in | Journal of liquid chromatography & related technologies Vol. 26; no. 15; pp. 2567 - 2579 |
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Main Authors | , |
Format | Journal Article |
Language | English |
Published |
Colchester
Taylor & Francis Group
01.08.2003
Taylor & Francis |
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Abstract | A fast and sensitive high performance liquid chromatography (HPLC) assay was developed for the simultaneous determination of aspirin-butalbital in human serum. Serum samples were treated with a solid phase extraction procedure. The analytes were separated using a mobile phase of 90:10 (v/v) 0.1 M aqueous potassium phosphate monobasic (pH 4)-acetonitrile on a monolithic C
18
column with UV detection at 220 nm. Benzoic acid was used as the internal standard (IS). The method was validated over the range of 0.5-100 µg mL
−1
for each drug. The method proved to be accurate (percent bias for all calibration samples varied from −13 to 6.6%) and precise (range from 0.1% to 10%). The mean percent absolute recoveries were 104 ± 6.3 for aspirin, 92.6 ± 5.5 for butalbital and 106 ± 6.9 for the internal standard. The assay should be applicable for use in pharmacokinetic studies and routine serum monitoring of these drugs. |
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AbstractList | A fast and sensitive high performance liquid chromatography (HPLC) assay was developed for the simultaneous determination of aspirin-butalbital in human serum. Serum samples were treated with a solid phase extraction procedure. The analytes were separated using a mobile phase of 90:10 (v/v) 0.1 M aqueous potassium phosphate monobasic (pH 4)-acetonitrile on a monolithic C
18
column with UV detection at 220 nm. Benzoic acid was used as the internal standard (IS). The method was validated over the range of 0.5-100 µg mL
−1
for each drug. The method proved to be accurate (percent bias for all calibration samples varied from −13 to 6.6%) and precise (range from 0.1% to 10%). The mean percent absolute recoveries were 104 ± 6.3 for aspirin, 92.6 ± 5.5 for butalbital and 106 ± 6.9 for the internal standard. The assay should be applicable for use in pharmacokinetic studies and routine serum monitoring of these drugs. |
Author | Pistos, C. Stewart, J. T. |
Author_xml | – sequence: 1 givenname: C. surname: Pistos fullname: Pistos, C. organization: Department of Pharmaceutical and Biomedical Sciences, College of Pharmacy , The University of Georgia – sequence: 2 givenname: J. T. surname: Stewart fullname: Stewart, J. T. email: jstewart@rx.uga.edu organization: Department of Pharmaceutical and Biomedical Sciences, College of Pharmacy , The University of Georgia |
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Keywords | Human Solid phase extraction Biological fluid Chemical analysis Monolithic column HPLC chromatography Hypnotic Acetylsalicylic acid Blood Non steroidal antiinflammatory agent Butalbital Reversed phase chromatography Analgesic Ultraviolet detector Sample preparation Simultaneous measurement Antipyretic Salicylates Sedative Barbiturates Blood serum Quantitative analysis |
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SubjectTerms | Analysis Aspirin-butalbital mixture Biological and medical sciences column General pharmacology HPLC Human serum Medical sciences Monolithic C Pharmacology. Drug treatments |
Title | Rapid Method for Analysis of Aspirin-Butalbital in Serum Utilizing a Monolithic C18 Column |
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