Role of Transforming Growth Factor-β1 (T29C) Gene Polymorphism in Hepatic Stellate Cell Activation and Invasion and Susceptibility to Hepatitis B, C Infection in an Iraqi Patients

• Published in: Journal of physics. Conference series Vol. 1294; no. 6
• Main Authors: AbdAlrazaaq AbdAlredha, Redhaa, Wahab Al-Kaim, Hawraa, Hussein Al-Marzoqi, Ali
• Format: Journal Article
• Language: English
• Published: Bristol IOP Publishing 01.09.2019
• Subjects: Cytokines; Females; Gastrointestinal system; Genetic variations; Growth factors; HBV; HCV; Hepatitis B; Hepatitis C; Infections; Liver; Liver diseases; Males; Pathogenesis; Physics; Polymorphism; TGF-β1 (T29C); Iraq
• DOI: 10.1088/1742-6596/1294/6/062012
• ISSN: 1742-6588

The interindividual varieties in the limit of changing development factor-β1 (TGF-β1) generation have been credited to hereditary polymorphisms in TGF-β1 quality. Changing development factor-beta 1 (TGFβ1) is a powerful suppressive cytokine that adds to ceaseless hepatitis B (CHB) disease. Abberations in TGFβ1 generation among people have been ascribed to TGFβ1 hereditary polymorphisms. We analyzed whether three putative polymorphisms in TGFβ1 (- 509 C/T (rs1800469), +869 C/T (rs1800470), and +11929 C/T (rs1800472)) square measure connected with CHB illness during a South-Eastern Iranian people.Methods:A total of 203 subjects with hepatitis infection (94 patients with hepatitis B virus infection and 109 patients with hepatitis C virus infection) whom admitted to Margan hospital, Center of liver diseases and gastrointestinal system were enrolled in the study. Allele specific (AS)-PCR, methods were used for assessing polymorphism of IL-10. Patients included (130 males and 73 females), with an age range (HBV: 44.6 ± 8.2), (HCV: 45.3±13.3) and (Control: 49.2 ± 9.04) years. The practical side of this study was done during the period from October 2017 to March 2018.Methods:A total of 203 subjects with hepatitis infection (94 patients with hepatitis B virus infection and 109 patients with hepatitis C virus infection) whom admitted to Margan hospital, Center of liver diseases and gastrointestinal system were enrolled in the study. Patients included (130 males and 73 females), with an age range (HBV: 44.6 ± 8.2), (HCV: 45.3±13.3) and (Con trol: 49.2 ± 9.04) years. The practical side of this study was done during the period from October 2017 to March 2018. As pathogenesis of HBV and HCV has a hereditary foundation, this primer investigation was intended to evaluate the effect of TGF-β1 (T29C) on the helplessness of Iraqi to HBV and HCV disease. Genotyping was performed utilizing single stranded polymorphism-polymerase chain response (SSP-PCR). Results: TGF-β1 T29Cgenetic polymorphism related to hepatitis B and C virus infection; revealed that the Genotype frequency of polymorphisms of (TGF) gene in Hepatitis B, C and Control, it was revealed that TC allele was higher than others 56.10% in control, 54.26% in HBV and 55.96% in HCV respectively. Results of Allele frequency showed that T allele was higher than C (69.51% in control, 57.98% HBV and 61.01% HCV).