Sense transcription through the S region is essential for immunoglobulin class switch recombination

• Published in: The EMBO journal Vol. 30; no. 8; pp. 1608 - 1620
• Main Authors: Haddad, Dania, Oruc, Zéliha, Puget, Nadine, Laviolette-Malirat, Nathalie, Philippe, Magali, Carrion, Claire, Le Bert, Marc, Khamlichi, Ahmed Amine
• Format: Journal Article
• Language: English
• Published: Chichester, UK John Wiley & Sons, Ltd 20.04.2011; Nature Publishing Group UK; Springer Nature B.V; EMBO Press; Nature Publishing Group
• Subjects: AID targeting; Animals; B cell; B-Lymphocytes - physiology; Cells, Cultured; class switching; Cytidine Deaminase - genetics; Deoxyribonucleic acid; DNA; DNA, Antisense - genetics; EMBO19; Enzyme-Linked Immunosorbent Assay; Flow Cytometry; germ-line transcription; Immunoglobulin Class Switching - genetics; Immunoglobulin Switch Region - genetics; Immunoglobulins; Immunoglobulins - genetics; Immunology; Life Sciences; Mice; Polyadenylation; Polymerase Chain Reaction; Recombination, Genetic; Rodents; Transcription, Genetic; AID targeting; class switching; B cell; germ‐line transcription

Class switch recombination (CSR) occurs between highly repetitive sequences called switch (S) regions and is initiated by activation‐induced cytidine deaminase (AID). CSR is preceded by a bidirectional transcription of S regions but the relative importance of sense and antisense transcription for CS...