ER–plasma membrane contact sites contribute to autophagosome biogenesis by regulation of local PI3P synthesis

The double‐membrane‐bound autophagosome is formed by the closure of a structure called the phagophore, origin of which is still unclear. The endoplasmic reticulum (ER) is clearly implicated in autophagosome biogenesis due to the presence of the omegasome subdomain positive for DFCP1, a phosphatidyl‐...

Full description

Saved in:
Bibliographic Details
Published inThe EMBO journal Vol. 36; no. 14; pp. 2018 - 2033
Main Authors Nascimbeni, Anna Chiara, Giordano, Francesca, Dupont, Nicolas, Grasso, Daniel, Vaccaro, Maria I, Codogno, Patrice, Morel, Etienne
Format Journal Article
LanguageEnglish
Published London Nature Publishing Group UK 14.07.2017
Springer Nature B.V
EMBO Press
John Wiley and Sons Inc
Subjects
Animals
autophagosome
Autophagosomes - metabolism
Autophagy
Biosynthesis
Carrier Proteins - metabolism
Cell Membrane - metabolism
Cellular Biology
contact sites
Cortex
Dogs
EMBO07
EMBO20
Endoplasmic reticulum
Endoplasmic Reticulum - metabolism
extended synaptotagmins
HeLa Cells
Humans
Life Sciences
Madin Darby Canine Kidney Cells
Markers
Membrane proteins
Membrane Proteins - metabolism
Membranes
Organelle Biogenesis
Phagocytosis
Phosphate
Phosphatidylinositol Phosphates - metabolism
PI3P
Plasma
Proteins
Recruitment
Synaptotagmins - metabolism
Synthesis
Tethering
Tethers
contact sites
organelle biogenesis
extended synaptotagmins
PI3P
autophagosome
PI3P Subject Categories Autophagy & Cell Death
Membrane & Intracellular
Online AccessGet full text

Cover

Abstract The double‐membrane‐bound autophagosome is formed by the closure of a structure called the phagophore, origin of which is still unclear. The endoplasmic reticulum (ER) is clearly implicated in autophagosome biogenesis due to the presence of the omegasome subdomain positive for DFCP1, a phosphatidyl‐inositol‐3‐phosphate (PI3P) binding protein. Contribution of other membrane sources, like the plasma membrane (PM), is still difficult to integrate in a global picture. Here we show that ER–plasma membrane contact sites are mobilized for autophagosome biogenesis, by direct implication of the tethering extended synaptotagmins (E‐Syts) proteins. Imaging data revealed that early autophagic markers are recruited to E‐Syt‐containing domains during autophagy and that inhibition of E‐Syts expression leads to a reduction in autophagosome biogenesis. Furthermore, we demonstrate that E‐Syts are essential for autophagy‐associated PI3P synthesis at the cortical ER membrane via the recruitment of VMP1, the stabilizing ER partner of the PI3KC3 complex. These results highlight the contribution of ER–plasma membrane tethers to autophagosome biogenesis regulation and support the importance of membrane contact sites in autophagy. Synopsis Early autophagic markers are recruited to endoplasmic reticulum‐plasma membrane (ER‐PM) contact sites established by tethering factors extended synaptotagmins, allowing for local phosphatidylinositol‐3‐phosphate synthesis and autophagosome biogenesis. Autophagy induction is accompanied by ER‐PM contact site mobilization. E‐Syt2, a major tethering protein of ER‐PM contact sites, forms a complex with VMP1 and Beclin1, two regulators of PI3KC3 complex activity. Local autophagosome biogenesis is initiated by local PI3P synthesis via the targeting of PI3KC3 complex at ER‐PM contact sites. Graphical Abstract Early autophagic markers are recruited to endoplasmic reticulum‐plasma membrane (ER‐PM) contact sites established by tethering factors extended synaptotagmins, allowing for local phosphatidylinositol‐3‐phosphate synthesis and autophagosome biogenesis.
AbstractList The double-membrane-bound autophagosome is formed by the closure of a structure called the phagophore, origin of which is still unclear. The endoplasmic reticulum (ER) is clearly implicated in autophagosome biogenesis due to the presence of the omegasome subdomain positive for DFCP1, a phosphatidyl-inositol-3-phosphate (PI3P) binding protein. Contribution of other membrane sources, like the plasma membrane (PM), is still difficult to integrate in a global picture. Here we show that ER–plasma membrane contact sites are mobilized for autophagosome biogenesis, by direct implication of the tethering extended synaptotagmins (E-Syts) proteins. Imaging data revealed that early autophagic markers are recruited to E-Syt-containing domains during autophagy and that inhibition of E-Syts expression leads to a reduction in autophagosome biogenesis. Furthermore, we demonstrate that E-Syts are essential for autophagy-associated PI3P synthesis at the cortical ER membrane via the recruitment of VMP1, the stabilizing ER partner of the PI3KC3 complex. These results highlight the contribution of ER–plasma membrane tethers to autophagosome biogenesis regulation and support the importance of membrane contact sites in autophagy.
The double‐membrane‐bound autophagosome is formed by the closure of a structure called the phagophore, origin of which is still unclear. The endoplasmic reticulum ( ER ) is clearly implicated in autophagosome biogenesis due to the presence of the omegasome subdomain positive for DFCP 1, a phosphatidyl‐inositol‐3‐phosphate ( PI 3P) binding protein. Contribution of other membrane sources, like the plasma membrane ( PM ), is still difficult to integrate in a global picture. Here we show that ER –plasma membrane contact sites are mobilized for autophagosome biogenesis, by direct implication of the tethering extended synaptotagmins (E‐Syts) proteins. Imaging data revealed that early autophagic markers are recruited to E‐Syt‐containing domains during autophagy and that inhibition of E‐Syts expression leads to a reduction in autophagosome biogenesis. Furthermore, we demonstrate that E‐Syts are essential for autophagy‐associated PI 3P synthesis at the cortical ER membrane via the recruitment of VMP 1, the stabilizing ER partner of the PI 3 KC 3 complex. These results highlight the contribution of ER –plasma membrane tethers to autophagosome biogenesis regulation and support the importance of membrane contact sites in autophagy.
The double-membrane-bound autophagosome is formed by the closure of a structure called the phagophore, origin of which is still unclear. The endoplasmic reticulum (ER) is clearly implicated in autophagosome biogenesis due to the presence of the omegasome subdomain positive for DFCP1, a phosphatidyl-inositol-3-phosphate (PI3P) binding protein. Contribution of other membrane sources, like the plasma membrane (PM), is still difficult to integrate in a global picture. Here we show that ER-plasma membrane contact sites are mobilized for autophagosome biogenesis, by direct implication of the tethering extended synaptotagmins (E-Syts) proteins. Imaging data revealed that early autophagic markers are recruited to E-Syt-containing domains during autophagy and that inhibition of E-Syts expression leads to a reduction in autophagosome biogenesis. Furthermore, we demonstrate that E-Syts are essential for autophagy-associated PI3P synthesis at the cortical ER membrane via the recruitment of VMP1, the stabilizing ER partner of the PI3KC3 complex. These results highlight the contribution of ER-plasma membrane tethers to autophagosome biogenesis regulation and support the importance of membrane contact sites in autophagy. Synopsis Early autophagic markers are recruited to endoplasmic reticulum-plasma membrane (ER-PM) contact sites established by tethering factors extended synaptotagmins, allowing for local phosphatidylinositol-3-phosphate synthesis and autophagosome biogenesis. Autophagy induction is accompanied by ER-PM contact site mobilization. E-Syt2, a major tethering protein of ER-PM contact sites, forms a complex with VMP1 and Beclin1, two regulators of PI3KC3 complex activity. Local autophagosome biogenesis is initiated by local PI3P synthesis via the targeting of PI3KC3 complex at ER-PM contact sites.
The double‐membrane‐bound autophagosome is formed by the closure of a structure called the phagophore, origin of which is still unclear. The endoplasmic reticulum (ER) is clearly implicated in autophagosome biogenesis due to the presence of the omegasome subdomain positive for DFCP1, a phosphatidyl‐inositol‐3‐phosphate (PI3P) binding protein. Contribution of other membrane sources, like the plasma membrane (PM), is still difficult to integrate in a global picture. Here we show that ER–plasma membrane contact sites are mobilized for autophagosome biogenesis, by direct implication of the tethering extended synaptotagmins (E‐Syts) proteins. Imaging data revealed that early autophagic markers are recruited to E‐Syt‐containing domains during autophagy and that inhibition of E‐Syts expression leads to a reduction in autophagosome biogenesis. Furthermore, we demonstrate that E‐Syts are essential for autophagy‐associated PI3P synthesis at the cortical ER membrane via the recruitment of VMP1, the stabilizing ER partner of the PI3KC3 complex. These results highlight the contribution of ER–plasma membrane tethers to autophagosome biogenesis regulation and support the importance of membrane contact sites in autophagy. Synopsis Early autophagic markers are recruited to endoplasmic reticulum‐plasma membrane (ER‐PM) contact sites established by tethering factors extended synaptotagmins, allowing for local phosphatidylinositol‐3‐phosphate synthesis and autophagosome biogenesis. Autophagy induction is accompanied by ER‐PM contact site mobilization. E‐Syt2, a major tethering protein of ER‐PM contact sites, forms a complex with VMP1 and Beclin1, two regulators of PI3KC3 complex activity. Local autophagosome biogenesis is initiated by local PI3P synthesis via the targeting of PI3KC3 complex at ER‐PM contact sites. Graphical Abstract Early autophagic markers are recruited to endoplasmic reticulum‐plasma membrane (ER‐PM) contact sites established by tethering factors extended synaptotagmins, allowing for local phosphatidylinositol‐3‐phosphate synthesis and autophagosome biogenesis.
The double-membrane-bound autophagosome is formed by the closure of a structure called the phagophore, origin of which is still unclear. The endoplasmic reticulum (ER) is clearly implicated in autophagosome biogenesis due to the presence of the omegasome subdomain positive for DFCP1, a phosphatidyl-inositol-3-phosphate (PI3P) binding protein. Contribution of other membrane sources, like the plasma membrane (PM), is still difficult to integrate in a global picture. Here we show that ER-plasma membrane contact sites are mobilized for autophagosome biogenesis, by direct implication of the tethering extended synaptotagmins (E-Syts) proteins. Imaging data revealed that early autophagic markers are recruited to E-Syt-containing domains during autophagy and that inhibition of E-Syts expression leads to a reduction in autophagosome biogenesis. Furthermore, we demonstrate that E-Syts are essential for autophagy-associated PI3P synthesis at the cortical ER membrane via the recruitment of VMP1, the stabilizing ER partner of the PI3KC3 complex. These results highlight the contribution of ER-plasma membrane tethers to autophagosome biogenesis regulation and support the importance of membrane contact sites in autophagy.The double-membrane-bound autophagosome is formed by the closure of a structure called the phagophore, origin of which is still unclear. The endoplasmic reticulum (ER) is clearly implicated in autophagosome biogenesis due to the presence of the omegasome subdomain positive for DFCP1, a phosphatidyl-inositol-3-phosphate (PI3P) binding protein. Contribution of other membrane sources, like the plasma membrane (PM), is still difficult to integrate in a global picture. Here we show that ER-plasma membrane contact sites are mobilized for autophagosome biogenesis, by direct implication of the tethering extended synaptotagmins (E-Syts) proteins. Imaging data revealed that early autophagic markers are recruited to E-Syt-containing domains during autophagy and that inhibition of E-Syts expression leads to a reduction in autophagosome biogenesis. Furthermore, we demonstrate that E-Syts are essential for autophagy-associated PI3P synthesis at the cortical ER membrane via the recruitment of VMP1, the stabilizing ER partner of the PI3KC3 complex. These results highlight the contribution of ER-plasma membrane tethers to autophagosome biogenesis regulation and support the importance of membrane contact sites in autophagy.
The double‐membrane‐bound autophagosome is formed by the closure of a structure called the phagophore, origin of which is still unclear. The endoplasmic reticulum (ER) is clearly implicated in autophagosome biogenesis due to the presence of the omegasome subdomain positive for DFCP1, a phosphatidyl‐inositol‐3‐phosphate (PI3P) binding protein. Contribution of other membrane sources, like the plasma membrane (PM), is still difficult to integrate in a global picture. Here we show that ER–plasma membrane contact sites are mobilized for autophagosome biogenesis, by direct implication of the tethering extended synaptotagmins (E‐Syts) proteins. Imaging data revealed that early autophagic markers are recruited to E‐Syt‐containing domains during autophagy and that inhibition of E‐Syts expression leads to a reduction in autophagosome biogenesis. Furthermore, we demonstrate that E‐Syts are essential for autophagy‐associated PI3P synthesis at the cortical ER membrane via the recruitment of VMP1, the stabilizing ER partner of the PI3KC3 complex. These results highlight the contribution of ER–plasma membrane tethers to autophagosome biogenesis regulation and support the importance of membrane contact sites in autophagy. Synopsis Early autophagic markers are recruited to endoplasmic reticulum‐plasma membrane (ER‐PM) contact sites established by tethering factors extended synaptotagmins, allowing for local phosphatidylinositol‐3‐phosphate synthesis and autophagosome biogenesis. Autophagy induction is accompanied by ER‐PM contact site mobilization. E‐Syt2, a major tethering protein of ER‐PM contact sites, forms a complex with VMP1 and Beclin1, two regulators of PI3KC3 complex activity. Local autophagosome biogenesis is initiated by local PI3P synthesis via the targeting of PI3KC3 complex at ER‐PM contact sites. Early autophagic markers are recruited to endoplasmic reticulum‐plasma membrane (ER‐PM) contact sites established by tethering factors extended synaptotagmins, allowing for local phosphatidylinositol‐3‐phosphate synthesis and autophagosome biogenesis.
Author Dupont, Nicolas
Vaccaro, Maria I
Nascimbeni, Anna Chiara
Codogno, Patrice
Morel, Etienne
Grasso, Daniel
Giordano, Francesca
AuthorAffiliation 2 Université Paris Descartes‐Sorbonne Paris Cité Paris France
1 Institut Necker‐Enfants Malades (INEM) INSERM U1151‐CNRS UMR 8253 Paris France
5 Department of Pathophysiology Institute of Biochemistry and Molecular Medicine National Council for Scientific and Technological Research School of Pharmacy and Biochemistry University of Buenos Aires Buenos Aires Argentina
4 Université Paris Diderot‐Sorbonne Paris Cité Paris France
3 Institut Jacques Monod CNRS UMR 7592 Paris France
AuthorAffiliation_xml – name: 1 Institut Necker‐Enfants Malades (INEM) INSERM U1151‐CNRS UMR 8253 Paris France
– name: 5 Department of Pathophysiology Institute of Biochemistry and Molecular Medicine National Council for Scientific and Technological Research School of Pharmacy and Biochemistry University of Buenos Aires Buenos Aires Argentina
– name: 3 Institut Jacques Monod CNRS UMR 7592 Paris France
– name: 4 Université Paris Diderot‐Sorbonne Paris Cité Paris France
– name: 2 Université Paris Descartes‐Sorbonne Paris Cité Paris France
Author_xml – sequence: 1
  givenname: Anna Chiara
  surname: Nascimbeni
  fullname: Nascimbeni, Anna Chiara
  organization: Institut Necker‐Enfants Malades (INEM), INSERM U1151‐CNRS UMR 8253, Université Paris Descartes‐Sorbonne Paris Cité
– sequence: 2
  givenname: Francesca
  orcidid: 0000-0002-5942-1753
  surname: Giordano
  fullname: Giordano, Francesca
  organization: Institut Jacques Monod, CNRS UMR 7592, Université Paris Diderot‐Sorbonne Paris Cité
– sequence: 3
  givenname: Nicolas
  surname: Dupont
  fullname: Dupont, Nicolas
  organization: Institut Necker‐Enfants Malades (INEM), INSERM U1151‐CNRS UMR 8253, Université Paris Descartes‐Sorbonne Paris Cité
– sequence: 4
  givenname: Daniel
  surname: Grasso
  fullname: Grasso, Daniel
  organization: Department of Pathophysiology, Institute of Biochemistry and Molecular Medicine, National Council for Scientific and Technological Research, School of Pharmacy and Biochemistry, University of Buenos Aires
– sequence: 5
  givenname: Maria I
  surname: Vaccaro
  fullname: Vaccaro, Maria I
  organization: Department of Pathophysiology, Institute of Biochemistry and Molecular Medicine, National Council for Scientific and Technological Research, School of Pharmacy and Biochemistry, University of Buenos Aires
– sequence: 6
  givenname: Patrice
  surname: Codogno
  fullname: Codogno, Patrice
  organization: Institut Necker‐Enfants Malades (INEM), INSERM U1151‐CNRS UMR 8253, Université Paris Descartes‐Sorbonne Paris Cité
– sequence: 7
  givenname: Etienne
  orcidid: 0000-0002-4763-4954
  surname: Morel
  fullname: Morel, Etienne
  email: etienne.morel@inserm.fr
  organization: Institut Necker‐Enfants Malades (INEM), INSERM U1151‐CNRS UMR 8253, Université Paris Descartes‐Sorbonne Paris Cité
BackLink https://www.ncbi.nlm.nih.gov/pubmed/28550152$$D View this record in MEDLINE/PubMed
https://inserm.hal.science/inserm-01529671$$DView record in HAL
BookMark eNptks9u1DAQxi1URLeFMzdkiQsH0nr8LzEHpFJtaatFVAjOlpM6iVeJvY2Tor3xDrwhT1Jvt6xKxckazW8-z3wzB2jPB28Reg3kCAQV9Nj25fKIEshVToh8hmbAJckoycUemhEqIeNQqH10EOOSECKKHF6gfVoIQZLADIX5tz-_fq86E3uD-6Q2GG9xFfxoqhFHN9p4Hw2unEaLx4DNNIZVa5oQQ29x6UJjvY0u4nKNB9tMnRld8DjUuAuV6fDVBbvCce3HdkO9RM9r00X76uE9RD_O5t9Pz7PF188XpyeLrOWUycyoAijlpOKy4OzaGM54DWXJuaq5MnnNhZIESk45AC_r3AiWZiaM1UbWjLFD9HGru5rK3l5XNo1gOr0aXG-GtQ7G6X8z3rW6Cbc6GaOUkkng_VagfVJ2frLQzkc79HpjoZI53ELC3z38N4SbycZR9y5WtuuSnWGKGhRhIIEJkdC3T9BlmAaf3EgUFGmTRc4T9ebxALsW_q4uAR-2wE_X2fUuD0TfX4beXIbeXYaef_l0uYtSMdkWx1TnGzs86uH_AuwOiMe_HQ
ContentType Journal Article
Copyright The Authors 2017
2017 The Authors
2017 The Authors.
2017 EMBO
Distributed under a Creative Commons Attribution 4.0 International License
Copyright_xml – notice: The Authors 2017
– notice: 2017 The Authors
– notice: 2017 The Authors.
– notice: 2017 EMBO
– notice: Distributed under a Creative Commons Attribution 4.0 International License
DBID CGR
CUY
CVF
ECM
EIF
NPM
7QG
7QL
7QP
7T5
7TK
7TM
7TO
7U9
8FD
C1K
FR3
H94
K9.
M7N
P64
RC3
7X8
1XC
VOOES
5PM
DOI 10.15252/embj.201797006
DatabaseName Medline
MEDLINE
MEDLINE (Ovid)
MEDLINE
MEDLINE
PubMed
Animal Behavior Abstracts
Bacteriology Abstracts (Microbiology B)
Calcium & Calcified Tissue Abstracts
Immunology Abstracts
Neurosciences Abstracts
Nucleic Acids Abstracts
Oncogenes and Growth Factors Abstracts
Virology and AIDS Abstracts
Technology Research Database
Environmental Sciences and Pollution Management
Engineering Research Database
AIDS and Cancer Research Abstracts
ProQuest Health & Medical Complete (Alumni)
Algology Mycology and Protozoology Abstracts (Microbiology C)
Biotechnology and BioEngineering Abstracts
Genetics Abstracts
MEDLINE - Academic
Hyper Article en Ligne (HAL)
Hyper Article en Ligne (HAL) (Open Access)
PubMed Central (Full Participant titles)
DatabaseTitle MEDLINE
Medline Complete
MEDLINE with Full Text
PubMed
MEDLINE (Ovid)
Virology and AIDS Abstracts
Oncogenes and Growth Factors Abstracts
Technology Research Database
Nucleic Acids Abstracts
ProQuest Health & Medical Complete (Alumni)
Neurosciences Abstracts
Biotechnology and BioEngineering Abstracts
Environmental Sciences and Pollution Management
Genetics Abstracts
Animal Behavior Abstracts
Bacteriology Abstracts (Microbiology B)
Algology Mycology and Protozoology Abstracts (Microbiology C)
AIDS and Cancer Research Abstracts
Immunology Abstracts
Engineering Research Database
Calcium & Calcified Tissue Abstracts
MEDLINE - Academic
DatabaseTitleList
MEDLINE

Virology and AIDS Abstracts

MEDLINE - Academic
Database_xml – sequence: 1
  dbid: NPM
  name: PubMed
  url: https://proxy.k.utb.cz/login?url=http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed
  sourceTypes: Index Database
– sequence: 2
  dbid: EIF
  name: MEDLINE
  url: https://proxy.k.utb.cz/login?url=https://www.webofscience.com/wos/medline/basic-search
  sourceTypes: Index Database
DeliveryMethod fulltext_linktorsrc
Discipline Chemistry
Biology
DocumentTitleAlternate Anna Chiara Nascimbeni et al
EISSN 1460-2075
EndPage 2033
ExternalDocumentID PMC5509996
oai_HAL_inserm_01529671v1
28550152
EMBJ201797006
10_15252_embj_201797006
Genre article
Research Support, Non-U.S. Gov't
Journal Article
GrantInformation_xml – fundername: INSERM
  funderid: 10.13039/501100001677
– fundername: INCa
  funderid: 10.13039/501100006364
– fundername: Association pour la Recherche sur le Cancer (ARC)
  funderid: 10.13039/100007391
– fundername: CNRS
  funderid: 10.13039/501100004794
– fundername: ECOsSUD
– fundername: University Paris Descartes
  funderid: 10.13039/501100005413
– fundername: ANR
  funderid: 10.13039/501100001665
– fundername: INSERM
– fundername: Association pour la Recherche sur le Cancer (ARC)
– fundername: CNRS
– fundername: ANR
– fundername: INCa
– fundername: University Paris Descartes
GroupedDBID ---
-DZ
-Q-
-~X
0R~
123
1OC
24P
29G
2WC
33P
36B
39C
53G
5VS
70F
8R4
8R5
A8Z
AAESR
AAEVG
AAHBH
AAHHS
AAIHA
AAJSJ
AANLZ
AAONW
AASGY
AAXRX
AAYCA
AAZKR
ABCUV
ABLJU
ACAHQ
ACCFJ
ACCZN
ACGFO
ACGFS
ACNCT
ACPOU
ACPRK
ACXBN
ACXQS
ADBBV
ADEOM
ADKYN
ADMGS
ADOZA
ADXAS
ADZMN
AEEZP
AEGXH
AEIGN
AENEX
AEQDE
AEUYR
AFBPY
AFFNX
AFGKR
AFPWT
AFRAH
AFWVQ
AFZJQ
AHMBA
AIAGR
AIURR
AIWBW
AJBDE
ALAGY
ALIPV
ALMA_UNASSIGNED_HOLDINGS
ALUQN
AMBMR
AMYDB
AOIJS
AUFTA
AZBYB
AZFZN
AZVAB
BAWUL
BDRZF
BENPR
BFHJK
BMNLL
BMXJE
BRXPI
BTFSW
C6C
CS3
DCZOG
DIK
DPXWK
DRFUL
DRSTM
DU5
E3Z
EBD
EBLON
EBS
EJD
EMB
EMOBN
F5P
G-S
GROUPED_DOAJ
GX1
HH5
HK~
HYE
KQ8
LATKE
LEEKS
LITHE
LOXES
LUTES
LYRES
MEWTI
MRFUL
MRSTM
MSFUL
MSSTM
MVM
MXFUL
MXSTM
MY~
O9-
OK1
P2P
P2W
Q2X
R.K
RHF
RHI
RNS
ROL
RPM
SV3
TN5
TR2
WBKPD
WH7
WIH
WIK
WIN
WOHZO
WXSBR
WYJ
YSK
ZCA
ZZTAW
~KM
ABJNI
CGR
CUY
CVF
ECM
EIF
NPM
7QG
7QL
7QP
7T5
7TK
7TM
7TO
7U9
8FD
AAMMB
AASML
ABZEH
AEFGJ
AGXDD
AIDQK
AIDYY
C1K
FR3
H94
K9.
M7N
NAO
P64
RC3
7X8
1XC
VOOES
5PM
ID FETCH-LOGICAL-h4236-a9812240c46843daa434f1bb449f49a7f459601b424114bf7a53075033fa6f333
IEDL.DBID C6C
ISSN 0261-4189
1460-2075
IngestDate Thu Aug 21 17:55:28 EDT 2025
Fri May 09 12:19:01 EDT 2025
Fri Jul 11 01:14:54 EDT 2025
Fri Jul 25 10:31:53 EDT 2025
Wed Feb 19 02:30:23 EST 2025
Wed Jan 22 16:32:16 EST 2025
Fri Feb 21 02:36:41 EST 2025
IsDoiOpenAccess true
IsOpenAccess true
IsPeerReviewed true
IsScholarly true
Issue 14
Keywords contact sites
organelle biogenesis
extended synaptotagmins
PI3P
autophagosome
PI3P Subject Categories Autophagy & Cell Death
Membrane & Intracellular
Language English
License 2017 The Authors.
Distributed under a Creative Commons Attribution 4.0 International License: http://creativecommons.org/licenses/by/4.0
LinkModel DirectLink
MergedId FETCHMERGED-LOGICAL-h4236-a9812240c46843daa434f1bb449f49a7f459601b424114bf7a53075033fa6f333
Notes ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 14
content type line 23
ORCID 0000-0002-5942-1753
0000-0002-4763-4954
0000-0002-5492-3180
OpenAccessLink https://inserm.hal.science/inserm-01529671
PMID 28550152
PQID 1918797874
PQPubID 35985
PageCount 16
ParticipantIDs pubmedcentral_primary_oai_pubmedcentral_nih_gov_5509996
hal_primary_oai_HAL_inserm_01529671v1
proquest_miscellaneous_1903161355
proquest_journals_1918797874
pubmed_primary_28550152
wiley_primary_10_15252_embj_201797006_EMBJ201797006
springer_journals_10_15252_embj_201797006
PublicationCentury 2000
PublicationDate 14 July 2017
PublicationDateYYYYMMDD 2017-07-14
PublicationDate_xml – month: 07
  year: 2017
  text: 14 July 2017
  day: 14
PublicationDecade 2010
PublicationPlace London
PublicationPlace_xml – name: London
– name: England
– name: New York
– name: Hoboken
PublicationTitle The EMBO journal
PublicationTitleAbbrev EMBO J
PublicationTitleAlternate EMBO J
PublicationYear 2017
Publisher Nature Publishing Group UK
Springer Nature B.V
EMBO Press
John Wiley and Sons Inc
Publisher_xml – name: Nature Publishing Group UK
– name: Springer Nature B.V
– name: EMBO Press
– name: John Wiley and Sons Inc
References Nascimbeni, Codogno, Morel (CR32) 2017; 284
Rubinsztein, Shpilka, Elazar (CR41) 2012; 22
Di Paolo, De Camilli (CR6) 2006; 443
Ge, Melville, Zhang, Schekman (CR12) 2013; 2
Lamb, Yoshimori, Tooze (CR25) 2013; 14
Levine, Liu, Dong, Zhong (CR26) 2015; 25
Itakura, Kishi, Inoue, Mizushima (CR20) 2008; 19
Shpilka, Welter, Borovsky, Amar, Mari, Reggiori, Elazar (CR45) 2015; 34
Helle, Kanfer, Kolar, Lang, Michel, Kornmann (CR19) 2013; 1833
Orhon, Dupont, Zaidan, Boitez, Burtin, Schmitt, Capiod, Viau, Beau, Wolfgang Kuehn, Friedlander, Terzi, Codogno (CR33) 2016; 18
Dooley, Razi, Polson, Girardin, Wilson, Tooze (CR7) 2014; 55
Graef, Friedman, Graham, Babu, Nunnari (CR16) 2013; 24
Slot, Geuze (CR46) 2007; 2
Kimura, Noda, Yoshimori (CR23) 2007; 3
Khaldoun, Emond‐Boisjoly, Chateau, Carrière, Lacasa, Rousset, Demignot, Morel (CR22) 2014; 25
Geng, Nair, Yasumura‐Yorimitsu, Klionsky (CR13) 2010; 21
Wilson, Dooley, Tooze (CR52) 2014; 42
Seaman, McCaffery, Emr (CR43) 1998; 142
Fernández‐Busnadiego, Saheki, De Camilli (CR10) 2015; 112
Puri, Renna, Bento, Moreau, Rubinsztein (CR37) 2013; 154
Dupont, Chauhan, Arko‐Mensah, Castillo, Masedunskas, Weigert, Robenek, Proikas‐Cezanne, Deretic (CR8) 2014; 24
Friedman, Voeltz (CR11) 2011; 21
Calvo‐Garrido, King, Muñoz‐Braceras, Escalante (CR4) 2014; 15
Molejon, Ropolo, Lo, Boggio, Vaccaro (CR29) 2013; 3
Ravikumar, Moreau, Rubinsztein (CR39) 2010; 6
Liang, Jackson, Seaman, Brown, Kempkes, Hibshoosh, Levine (CR27) 1999; 402
Ravikumar, Moreau, Jahreiss, Puri, Rubinsztein (CR38) 2010; 12
Molino, Zemirli, Codogno, Morel (CR31) 2017; 429
Elbaz, Schuldiner (CR9) 2011; 36
Roberts, Ktistakis (CR40) 2013; 55
Carlos Martín Zoppino, Damián Militello, Slavin, Álvarez, Colombo (CR5) 2010; 11
Stefan, Manford, Emr (CR47) 2013; 25
Alers, Loffler, Wesselborg, Stork (CR1) 2012; 10
Boya, Reggiori, Codogno (CR3) 2013; 15
Tooze (CR51) 2013; 55
Hamasaki, Furuta, Matsuda, Nezu, Yamamoto, Fujita, Oomori, Noda, Haraguchi, Hiraoka, Amano, Yoshimori (CR18) 2013; 495
Marat, Haucke (CR28) 2016; 35
Powis, Bonjouklian, Berggren, Gallegos, Abraham, Ashendel, Zalkow, Matter, Dodge, Grindey (CR36) 1994; 54
Polson, de Lartigue, Rigden, Reedijk, Urbé, Clague, Tooze (CR35) 2010; 6
Itakura, Mizushima (CR21) 2010; 6
Pérez‐Lara, Jahn (CR34) 2015; 112
Tábara, Escalante (CR49) 2016; 11
Gomez‐Suaga, Paillusson, Stoica, Noble, Hanger, Miller (CR15) 2017; 27
Klionsky, Abdelmohsen, Abe, Abedin, Abeliovich, Acevedo Arozena, Adachi, Adams, Adams, Adeli, Adhihetty, Adler, Agam, Agarwal, Aghi, Agnello, Agostinis, Aguilar, Aguirre‐Ghiso, Airoldi (CR24) 2016; 12
Molejon, Ropolo, Vaccaro (CR30) 2013; 9
Liou, Chang (CR55) 2015; 14
Sun, Fan, Chen, Ding, Chen, Zhong (CR48) 2008; 105
Axe, Walker, Manifava, Chandra, Roderick, Habermann, Griffiths, Ktistakis (CR2) 2008; 182
Hailey, Rambold, Satpute‐Krishnan, Mitra, Sougrat, Kim, Lippincott‐Schwartz (CR17) 2010; 141
Giordano, Saheki, Idevall‐Hagren, Colombo, Pirruccello, Milosevic, Gracheva, Bagriantsev, Borgese, De Camilli (CR14) 2013; 153
Schauder, Wu, Saheki, Narayanaswamy, Torta, Wenk, De Camilli, Reinisch (CR42) 2014; 510
Shibata, Voss, Rist, Hu, Rapoport, Prinz, Voeltz (CR44) 2008; 283
2010; 11
2015; 34
2013; 25
2013; 2
2013; 24
2010; 141
2014; 25
2014; 24
2008; 105
1999; 402
2012; 10
2016; 35
2008; 182
2010; 21
2013; 15
2013; 14
2013; 55
2014; 15
2011; 21
2017; 284
2013; 154
2007; 2
2013; 153
2007; 3
2012; 22
2014; 55
2010; 6
2006; 443
2013; 1833
2015; 14
2010a; 12
2017; 27
2008; 19
2013a; 3
2011; 36
2016; 18
2010b; 6
2014; 510
2008; 283
2016; 12
2016; 11
2014; 42
2017; 429
2015; 25
2015; 112
2013b; 9
2013; 495
1994; 54
1998; 142
28559575 - Nat Rev Mol Cell Biol. 2017 Jul;18(7):404-405
References_xml – volume: 2
  start-page: 2480
  year: 2007
  end-page: 2491
  ident: CR46
  article-title: Cryosectioning and immunolabeling
  publication-title: Nat Protoc
– volume: 55
  start-page: 238
  year: 2014
  end-page: 252
  ident: CR7
  article-title: WIPI2 links LC3 conjugation with PI3P, autophagosome formation, and pathogen clearance by recruiting Atg12‐5‐16L1
  publication-title: Mol Cell
– volume: 35
  start-page: 561
  year: 2016
  end-page: 579
  ident: CR28
  article-title: Phosphatidylinositol 3‐phosphates‐at the interface between cell signalling and membrane traffic
  publication-title: EMBO J
– volume: 283
  start-page: 18892
  year: 2008
  end-page: 18904
  ident: CR44
  article-title: The reticulon and DP1/Yop1p proteins form immobile oligomers in the tubular endoplasmic reticulum
  publication-title: J Biol Chem
– volume: 36
  start-page: 616
  year: 2011
  end-page: 623
  ident: CR9
  article-title: Staying in touch: the molecular era of organelle contact sites
  publication-title: Trends Biochem Sci
– volume: 15
  start-page: 713
  year: 2013
  end-page: 720
  ident: CR3
  article-title: Emerging regulation and functions of autophagy
  publication-title: Nat Cell Biol
– volume: 18
  start-page: 657
  year: 2016
  end-page: 667
  ident: CR33
  article-title: Primary‐cilium‐dependent autophagy controls epithelial cell volume in response to fluid flow
  publication-title: Nat Cell Biol
– volume: 34
  start-page: 2117
  year: 2015
  end-page: 2131
  ident: CR45
  article-title: Lipid droplets and their component triglycerides and steryl esters regulate autophagosome biogenesis
  publication-title: EMBO J
– volume: 14
  start-page: 759
  year: 2013
  end-page: 774
  ident: CR25
  article-title: The autophagosome: origins unknown, biogenesis complex
  publication-title: Nat Rev Mol Cell Biol
– volume: 11
  start-page: 1246
  year: 2010
  end-page: 1261
  ident: CR5
  article-title: Autophagosome formation depends on the small GTPase Rab1 and functional ER exit sites
  publication-title: Traffic
– volume: 402
  start-page: 672
  year: 1999
  end-page: 676
  ident: CR27
  article-title: Induction of autophagy and inhibition of tumorigenesis by beclin 1
  publication-title: Nature
– volume: 3
  start-page: 1055
  year: 2013
  ident: CR29
  article-title: The VMP1‐Beclin 1 interaction regulates autophagy induction
  publication-title: Sci Rep
– volume: 6
  start-page: 1184
  year: 2010
  end-page: 1186
  ident: CR39
  article-title: Plasma membrane helps autophagosomes grow
  publication-title: Autophagy
– volume: 11
  start-page: e0166499
  year: 2016
  ident: CR49
  article-title: VMP1 establishes ER‐microdomains that regulate membrane contact sites and autophagy
  publication-title: PLoS One
– volume: 3
  start-page: 452
  year: 2007
  end-page: 460
  ident: CR23
  article-title: Dissection of the autophagosome maturation process by a novel reporter protein, tandem fluorescent‐tagged LC3
  publication-title: Autophagy
– volume: 25
  start-page: 434
  year: 2013
  end-page: 442
  ident: CR47
  article-title: ER‐PM connections: sites of information transfer and inter‐organelle communication
  publication-title: Curr Opin Cell Biol
– volume: 510
  start-page: 552
  year: 2014
  end-page: 555
  ident: CR42
  article-title: Structure of a lipid‐bound extended synaptotagmin indicates a role in lipid transfer
  publication-title: Nature
– volume: 25
  start-page: 533
  year: 2015
  end-page: 544
  ident: CR26
  article-title: Beclin orthologs: integrative hubs of cell signaling, membrane trafficking, and physiology
  publication-title: Trends Cell Biol
– volume: 153
  start-page: 1494
  year: 2013
  end-page: 1509
  ident: CR14
  article-title: PI(4,5)P(2)‐dependent and Ca(2+)‐regulated ER‐PM interactions mediated by the extended synaptotagmins
  publication-title: Cell
– volume: 24
  start-page: 2918
  year: 2013
  end-page: 2931
  ident: CR16
  article-title: ER exit sites are physical and functional core autophagosome biogenesis components
  publication-title: Mol Biol Cell
– volume: 495
  start-page: 389
  year: 2013
  end-page: 393
  ident: CR18
  article-title: Autophagosomes form at ER‐mitochondria contact sites
  publication-title: Nature
– volume: 1833
  start-page: 2526
  year: 2013
  end-page: 2541
  ident: CR19
  article-title: Organization and function of membrane contact sites
  publication-title: Biochim Biophys Acta
– volume: 42
  start-page: 1327
  year: 2014
  end-page: 1334
  ident: CR52
  article-title: WIPI2b and Atg16L1: setting the stage for autophagosome formation
  publication-title: Biochem Soc Trans
– volume: 429
  start-page: 497
  year: 2017
  end-page: 514
  ident: CR31
  article-title: The journey of the autophagosome through mammalian cell organelles and membranes
  publication-title: J Mol Biol
– volume: 9
  start-page: 933
  year: 2013
  end-page: 935
  ident: CR30
  article-title: VMP1 is a new player in the regulation of the autophagy‐specific phosphatidylinositol 3‐kinase complex activation
  publication-title: Autophagy
– volume: 142
  start-page: 665
  year: 1998
  end-page: 681
  ident: CR43
  article-title: A membrane coat complex essential for endosome‐to‐Golgi retrograde transport in yeast
  publication-title: J Cell Biol
– volume: 112
  start-page: E2004
  year: 2015
  end-page: E2013
  ident: CR10
  article-title: Three‐dimensional architecture of extended synaptotagmin‐mediated endoplasmic reticulum‐plasma membrane contact sites
  publication-title: Proc Natl Acad Sci USA
– volume: 14
  start-page: 799
  year: 2015
  end-page: 800
  ident: CR55
  article-title: Unveiling physiological functions of extended synaptotagmins
  publication-title: Cell Cycle
– volume: 22
  start-page: R29
  year: 2012
  end-page: R34
  ident: CR41
  article-title: Mechanisms of autophagosome biogenesis
  publication-title: Curr Biol
– volume: 443
  start-page: 651
  year: 2006
  end-page: 657
  ident: CR6
  article-title: Phosphoinositides in cell regulation and membrane dynamics
  publication-title: Nature
– volume: 25
  start-page: 118
  year: 2014
  end-page: 132
  ident: CR22
  article-title: Autophagosomes contribute to intracellular lipid distribution in enterocytes
  publication-title: Mol Biol Cell
– volume: 24
  start-page: 609
  year: 2014
  end-page: 620
  ident: CR8
  article-title: Neutral lipid stores and lipase PNPLA5 contribute to autophagosome biogenesis
  publication-title: Curr Biol
– volume: 19
  start-page: 5360
  year: 2008
  end-page: 5372
  ident: CR20
  article-title: Beclin 1 forms two distinct phosphatidylinositol 3‐kinase complexes with mammalian Atg14 and UVRAG
  publication-title: Mol Biol Cell
– volume: 141
  start-page: 656
  year: 2010
  end-page: 667
  ident: CR17
  article-title: Mitochondria supply membranes for autophagosome biogenesis during starvation
  publication-title: Cell
– volume: 54
  start-page: 2419
  year: 1994
  end-page: 2423
  ident: CR36
  article-title: Wortmannin, a potent and selective inhibitor of phosphatidylinositol‐3‐kinase
  publication-title: Cancer Res
– volume: 21
  start-page: 709
  year: 2011
  end-page: 717
  ident: CR11
  article-title: The ER in 3D: a multifunctional dynamic membrane network
  publication-title: Trends Cell Biol
– volume: 12
  start-page: 1
  year: 2016
  end-page: 222
  ident: CR24
  article-title: Guidelines for the use and interpretation of assays for monitoring autophagy (3rd edition)
  publication-title: Autophagy
– volume: 105
  start-page: 19211
  year: 2008
  end-page: 19216
  ident: CR48
  article-title: Identification of Barkor as a mammalian autophagy‐specific factor for Beclin 1 and class III phosphatidylinositol 3‐kinase
  publication-title: Proc Natl Acad Sci USA
– volume: 284
  start-page: 1267
  year: 2017
  end-page: 1278
  ident: CR32
  article-title: Phosphatidylinositol‐3‐phosphate in the regulation of autophagy membrane dynamics
  publication-title: FEBS J
– volume: 154
  start-page: 1285
  year: 2013
  end-page: 1299
  ident: CR37
  article-title: Diverse autophagosome membrane sources coalesce in recycling endosomes
  publication-title: Cell
– volume: 55
  start-page: 29
  year: 2013
  end-page: 38
  ident: CR51
  article-title: Current views on the source of the autophagosome membrane
  publication-title: Essays Biochem
– volume: 55
  start-page: 17
  year: 2013
  end-page: 27
  ident: CR40
  article-title: Omegasomes: PI3P platforms that manufacture autophagosomes
  publication-title: Essays Biochem
– volume: 2
  start-page: e00947
  year: 2013
  ident: CR12
  article-title: The ER‐Golgi intermediate compartment is a key membrane source for the LC3 lipidation step of autophagosome biogenesis
  publication-title: Elife
– volume: 12
  start-page: 747
  year: 2010
  end-page: 757
  ident: CR38
  article-title: Plasma membrane contributes to the formation of pre‐autophagosomal structures
  publication-title: Nat Cell Biol
– volume: 10
  start-page: 7
  year: 2012
  ident: CR1
  article-title: The incredible ULKs
  publication-title: Cell Commun Signal
– volume: 182
  start-page: 685
  year: 2008
  end-page: 701
  ident: CR2
  article-title: Autophagosome formation from membrane compartments enriched in phosphatidylinositol 3‐phosphate and dynamically connected to the endoplasmic reticulum
  publication-title: J Cell Biol
– volume: 112
  start-page: 4837
  year: 2015
  end-page: 4838
  ident: CR34
  article-title: Extended synaptotagmins (E‐Syts): architecture and dynamics of membrane contact sites revealed
  publication-title: Proc Natl Acad Sci USA
– volume: 15
  start-page: 1235
  year: 2014
  end-page: 1246
  ident: CR4
  article-title: Vmp1 regulates PtdIns3P signaling during autophagosome formation in
  publication-title: Traffic
– volume: 6
  start-page: 506
  year: 2010
  end-page: 522
  ident: CR35
  article-title: Mammalian Atg18 (WIPI2) localizes to omegasome‐anchored phagophores and positively regulates LC3 lipidation
  publication-title: Autophagy
– volume: 27
  start-page: 371
  year: 2017
  end-page: 385
  ident: CR15
  article-title: The ER‐mitochondria tethering complex VAPB‐PTPIP51 regulates autophagy
  publication-title: Curr Biol
– volume: 21
  start-page: 2257
  year: 2010
  end-page: 2269
  ident: CR13
  article-title: Post‐Golgi Sec proteins are required for autophagy in Saccharomyces cerevisiae
  publication-title: Mol Biol Cell
– volume: 6
  start-page: 764
  year: 2010
  end-page: 776
  ident: CR21
  article-title: Characterization of autophagosome formation site by a hierarchical analysis of mammalian Atg proteins
  publication-title: Autophagy
– volume: 141
  start-page: 656
  year: 2010
  end-page: 667
  article-title: Mitochondria supply membranes for autophagosome biogenesis during starvation
  publication-title: Cell
– volume: 22
  start-page: R29
  year: 2012
  end-page: R34
  article-title: Mechanisms of autophagosome biogenesis
  publication-title: Curr Biol
– volume: 510
  start-page: 552
  year: 2014
  end-page: 555
  article-title: Structure of a lipid‐bound extended synaptotagmin indicates a role in lipid transfer
  publication-title: Nature
– volume: 18
  start-page: 657
  year: 2016
  end-page: 667
  article-title: Primary‐cilium‐dependent autophagy controls epithelial cell volume in response to fluid flow
  publication-title: Nat Cell Biol
– volume: 12
  start-page: 747
  year: 2010a
  end-page: 757
  article-title: Plasma membrane contributes to the formation of pre‐autophagosomal structures
  publication-title: Nat Cell Biol
– volume: 42
  start-page: 1327
  year: 2014
  end-page: 1334
  article-title: WIPI2b and Atg16L1: setting the stage for autophagosome formation
  publication-title: Biochem Soc Trans
– volume: 6
  start-page: 764
  year: 2010
  end-page: 776
  article-title: Characterization of autophagosome formation site by a hierarchical analysis of mammalian Atg proteins
  publication-title: Autophagy
– volume: 3
  start-page: 1055
  year: 2013a
  article-title: The VMP1‐Beclin 1 interaction regulates autophagy induction
  publication-title: Sci Rep
– volume: 429
  start-page: 497
  year: 2017
  end-page: 514
  article-title: The journey of the autophagosome through mammalian cell organelles and membranes
  publication-title: J Mol Biol
– volume: 25
  start-page: 118
  year: 2014
  end-page: 132
  article-title: Autophagosomes contribute to intracellular lipid distribution in enterocytes
  publication-title: Mol Biol Cell
– volume: 11
  start-page: 1246
  year: 2010
  end-page: 1261
  article-title: Autophagosome formation depends on the small GTPase Rab1 and functional ER exit sites
  publication-title: Traffic
– volume: 11
  start-page: e0166499
  year: 2016
  article-title: VMP1 establishes ER‐microdomains that regulate membrane contact sites and autophagy
  publication-title: PLoS One
– volume: 3
  start-page: 452
  year: 2007
  end-page: 460
  article-title: Dissection of the autophagosome maturation process by a novel reporter protein, tandem fluorescent‐tagged LC3
  publication-title: Autophagy
– volume: 54
  start-page: 2419
  year: 1994
  end-page: 2423
  article-title: Wortmannin, a potent and selective inhibitor of phosphatidylinositol‐3‐kinase
  publication-title: Cancer Res
– volume: 14
  start-page: 759
  year: 2013
  end-page: 774
  article-title: The autophagosome: origins unknown, biogenesis complex
  publication-title: Nat Rev Mol Cell Biol
– volume: 36
  start-page: 616
  year: 2011
  end-page: 623
  article-title: Staying in touch: the molecular era of organelle contact sites
  publication-title: Trends Biochem Sci
– volume: 24
  start-page: 2918
  year: 2013
  end-page: 2931
  article-title: ER exit sites are physical and functional core autophagosome biogenesis components
  publication-title: Mol Biol Cell
– volume: 443
  start-page: 651
  year: 2006
  end-page: 657
  article-title: Phosphoinositides in cell regulation and membrane dynamics
  publication-title: Nature
– volume: 55
  start-page: 17
  year: 2013
  end-page: 27
  article-title: Omegasomes: PI3P platforms that manufacture autophagosomes
  publication-title: Essays Biochem
– volume: 283
  start-page: 18892
  year: 2008
  end-page: 18904
  article-title: The reticulon and DP1/Yop1p proteins form immobile oligomers in the tubular endoplasmic reticulum
  publication-title: J Biol Chem
– volume: 15
  start-page: 1235
  year: 2014
  end-page: 1246
  article-title: Vmp1 regulates PtdIns3P signaling during autophagosome formation in
  publication-title: Traffic
– volume: 142
  start-page: 665
  year: 1998
  end-page: 681
  article-title: A membrane coat complex essential for endosome‐to‐Golgi retrograde transport in yeast
  publication-title: J Cell Biol
– volume: 14
  start-page: 799
  year: 2015
  end-page: 800
  article-title: Unveiling physiological functions of extended synaptotagmins
  publication-title: Cell Cycle
– volume: 402
  start-page: 672
  year: 1999
  end-page: 676
  article-title: Induction of autophagy and inhibition of tumorigenesis by beclin 1
  publication-title: Nature
– volume: 2
  start-page: e00947
  year: 2013
  article-title: The ER‐Golgi intermediate compartment is a key membrane source for the LC3 lipidation step of autophagosome biogenesis
  publication-title: Elife
– volume: 105
  start-page: 19211
  year: 2008
  end-page: 19216
  article-title: Identification of Barkor as a mammalian autophagy‐specific factor for Beclin 1 and class III phosphatidylinositol 3‐kinase
  publication-title: Proc Natl Acad Sci USA
– volume: 35
  start-page: 561
  year: 2016
  end-page: 579
  article-title: Phosphatidylinositol 3‐phosphates‐at the interface between cell signalling and membrane traffic
  publication-title: EMBO J
– volume: 182
  start-page: 685
  year: 2008
  end-page: 701
  article-title: Autophagosome formation from membrane compartments enriched in phosphatidylinositol 3‐phosphate and dynamically connected to the endoplasmic reticulum
  publication-title: J Cell Biol
– volume: 21
  start-page: 709
  year: 2011
  end-page: 717
  article-title: The ER in 3D: a multifunctional dynamic membrane network
  publication-title: Trends Cell Biol
– volume: 19
  start-page: 5360
  year: 2008
  end-page: 5372
  article-title: Beclin 1 forms two distinct phosphatidylinositol 3‐kinase complexes with mammalian Atg14 and UVRAG
  publication-title: Mol Biol Cell
– volume: 6
  start-page: 1184
  year: 2010b
  end-page: 1186
  article-title: Plasma membrane helps autophagosomes grow
  publication-title: Autophagy
– volume: 55
  start-page: 238
  year: 2014
  end-page: 252
  article-title: WIPI2 links LC3 conjugation with PI3P, autophagosome formation, and pathogen clearance by recruiting Atg12‐5‐16L1
  publication-title: Mol Cell
– volume: 9
  start-page: 933
  year: 2013b
  end-page: 935
  article-title: VMP1 is a new player in the regulation of the autophagy‐specific phosphatidylinositol 3‐kinase complex activation
  publication-title: Autophagy
– volume: 154
  start-page: 1285
  year: 2013
  end-page: 1299
  article-title: Diverse autophagosome membrane sources coalesce in recycling endosomes
  publication-title: Cell
– volume: 112
  start-page: E2004
  year: 2015
  end-page: E2013
  article-title: Three‐dimensional architecture of extended synaptotagmin‐mediated endoplasmic reticulum‐plasma membrane contact sites
  publication-title: Proc Natl Acad Sci USA
– volume: 27
  start-page: 371
  year: 2017
  end-page: 385
  article-title: The ER‐mitochondria tethering complex VAPB‐PTPIP51 regulates autophagy
  publication-title: Curr Biol
– volume: 153
  start-page: 1494
  year: 2013
  end-page: 1509
  article-title: PI(4,5)P(2)‐dependent and Ca(2+)‐regulated ER‐PM interactions mediated by the extended synaptotagmins
  publication-title: Cell
– volume: 25
  start-page: 434
  year: 2013
  end-page: 442
  article-title: ER‐PM connections: sites of information transfer and inter‐organelle communication
  publication-title: Curr Opin Cell Biol
– volume: 55
  start-page: 29
  year: 2013
  end-page: 38
  article-title: Current views on the source of the autophagosome membrane
  publication-title: Essays Biochem
– volume: 6
  start-page: 506
  year: 2010
  end-page: 522
  article-title: Mammalian Atg18 (WIPI2) localizes to omegasome‐anchored phagophores and positively regulates LC3 lipidation
  publication-title: Autophagy
– volume: 34
  start-page: 2117
  year: 2015
  end-page: 2131
  article-title: Lipid droplets and their component triglycerides and steryl esters regulate autophagosome biogenesis
  publication-title: EMBO J
– volume: 21
  start-page: 2257
  year: 2010
  end-page: 2269
  article-title: Post‐Golgi Sec proteins are required for autophagy in Saccharomyces cerevisiae
  publication-title: Mol Biol Cell
– volume: 15
  start-page: 713
  year: 2013
  end-page: 720
  article-title: Emerging regulation and functions of autophagy
  publication-title: Nat Cell Biol
– volume: 25
  start-page: 533
  year: 2015
  end-page: 544
  article-title: Beclin orthologs: integrative hubs of cell signaling, membrane trafficking, and physiology
  publication-title: Trends Cell Biol
– volume: 2
  start-page: 2480
  year: 2007
  end-page: 2491
  article-title: Cryosectioning and immunolabeling
  publication-title: Nat Protoc
– volume: 12
  start-page: 1
  year: 2016
  end-page: 222
  article-title: Guidelines for the use and interpretation of assays for monitoring autophagy (3rd edition)
  publication-title: Autophagy
– volume: 284
  start-page: 1267
  year: 2017
  end-page: 1278
  article-title: Phosphatidylinositol‐3‐phosphate in the regulation of autophagy membrane dynamics
  publication-title: FEBS J
– volume: 10
  start-page: 7
  year: 2012
  article-title: The incredible ULKs
  publication-title: Cell Commun Signal
– volume: 495
  start-page: 389
  year: 2013
  end-page: 393
  article-title: Autophagosomes form at ER‐mitochondria contact sites
  publication-title: Nature
– volume: 24
  start-page: 609
  year: 2014
  end-page: 620
  article-title: Neutral lipid stores and lipase PNPLA5 contribute to autophagosome biogenesis
  publication-title: Curr Biol
– volume: 112
  start-page: 4837
  year: 2015
  end-page: 4838
  article-title: Extended synaptotagmins (E‐Syts): architecture and dynamics of membrane contact sites revealed
  publication-title: Proc Natl Acad Sci USA
– volume: 1833
  start-page: 2526
  year: 2013
  end-page: 2541
  article-title: Organization and function of membrane contact sites
  publication-title: Biochim Biophys Acta
– reference: 28559575 - Nat Rev Mol Cell Biol. 2017 Jul;18(7):404-405
SSID ssj0005871
Score 2.5841067
Snippet The double‐membrane‐bound autophagosome is formed by the closure of a structure called the phagophore, origin of which is still unclear. The endoplasmic...
The double-membrane-bound autophagosome is formed by the closure of a structure called the phagophore, origin of which is still unclear. The endoplasmic...
SourceID pubmedcentral
hal
proquest
pubmed
wiley
springer
SourceType Open Access Repository
Aggregation Database
Index Database
Publisher
StartPage 2018
SubjectTerms Animals
autophagosome
Autophagosomes - metabolism
Autophagy
Biosynthesis
Carrier Proteins - metabolism
Cell Membrane - metabolism
Cellular Biology
contact sites
Cortex
Dogs
EMBO07
EMBO20
Endoplasmic reticulum
Endoplasmic Reticulum - metabolism
extended synaptotagmins
HeLa Cells
Humans
Life Sciences
Madin Darby Canine Kidney Cells
Markers
Membrane proteins
Membrane Proteins - metabolism
Membranes
Organelle Biogenesis
Phagocytosis
Phosphate
Phosphatidylinositol Phosphates - metabolism
PI3P
Plasma
Proteins
Recruitment
Synaptotagmins - metabolism
Synthesis
Tethering
Tethers
Title ER–plasma membrane contact sites contribute to autophagosome biogenesis by regulation of local PI3P synthesis
URI https://link.springer.com/article/10.15252/embj.201797006
https://onlinelibrary.wiley.com/doi/abs/10.15252%2Fembj.201797006
https://www.ncbi.nlm.nih.gov/pubmed/28550152
https://www.proquest.com/docview/1918797874
https://www.proquest.com/docview/1903161355
https://inserm.hal.science/inserm-01529671
https://pubmed.ncbi.nlm.nih.gov/PMC5509996
Volume 36
hasFullText 1
inHoldings 1
isFullTextHit
isPrint
link http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwlV3NbtQwELZQEYILgvIXKJWR4MAhYh3_Jcey2mqpKKoQlXqz7Kzd3UpJqmYXaW99h74hT8KMkw1dFQ6cEsuObXnGnm88kxlC3gfA2DMx4yk8bCpCKFOXO5V6J5nnCrOKoEX3-JuanoqjM3nWB0nCf2Fu2-9lJrNPvnIX6IGlCx1Da9-XjGvM0TBW4z--HHnUrOJlimB50cfw-UsHIELm6PF4F07e9YocTKPbwDVKnsMn5HEPGelBR-On5J6vd8mDLonkepc8HG9ytj0jzeT7r-ubS0DElaUVzAkkkafojm7LJUVDcRtLMcuVp8uG2hVGFrDnTdtUnrpFc46H36Klbk2vujz1QDnaBBqlHj35wk9ou64BN0Kr5-T0cPJjPE37lArpHHCTSm2RoyltVAqVCz6zVnARmHNCFEEUVgchQaVhToBgZ8IFbSXvTJ3BqsA5f0F26qb2rwjVs1EpnVRiZHNhS-XKMuOFt8zlhdTcJuQDrLS57IJmGAxjPT34ahY1bK3KAArJCqXZT5aQvQ0tTL9_WgNaZA4Ey7VIyLuhGhYTzRmwcs0K28CBBGhEyoS87Eg3jJZhnDYYIiF6i6hb09muqRfzGF0bPkQlMCEfN-S_NS3QmJCpDDKVGZgqITzyx9D7P9qZyfHno6H0-j9GeEMe4TteKjOxR3aWVyv_FtDQ0u3HnbCPEkn-BgPeA-E
linkProvider Springer Nature
linkToHtml http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwlV1fb9MwED9NndB4QTD-LGOAkeCBh4i6thPnsUydutJOE2zS3iw7ddYiJZmWdlLf-A58Qz4JZ-cPVIMHniLHjm35zr7f-S53AO8yxNhzPmchPnTIsywNjTRRaI2glkUuq4iz6M7OovEln1yJqx2g7b8w3tu9NUn6k7rJ0TP4aHPzzflixUnsg2zvShFJ0YPd4XDydfLbr0N6LctfrHAqkyaez1-6QHGycN6P96HlfQ_Jzky6DWK9FDp5DI8a-EiGNb2fwI4t9uFBnVBysw97x23-tqdQjr78_P7jBtFxrkmOc0KpZIlzTdfpijijceVLPuOVJauS6LWLMqCvy6rMLTHL8todhMuKmA25rXPWIxVJmREvAcn5KTsn1aZADImtnsHlyejieBw26RXCBWKoKNSJdGa1fsojydlca854Ro3hPMl4ouOMC1RvqOEo5Ck3WawFq82emY4yxthz6BVlYQ-AxPN-KoyIeF9LrtPIpOmAJVZTIxMRMx3Ae1xpdVMH0FAupPV4OFXLArdZrhCRDJIopnc0gKOWFqrZS5VCjVIiwWTMA3jbVeNiOtMGrly5dm3wcEJkIkQAL2rSdaMNXMw2HCKAeIuoW9PZrimWCx9pGz90CmEAH1ry_zEt1J4cUynHVKpjqgCY54-u93-0U6PZp0lXOvyPEd7A3vhiNlXT07PPL-Ghe-8umyk_gt7qdm1fIUpamdfNvvgFuccLXg
linkToPdf http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwlV1Rb9MwED6hIWAvCAaMwAAjwQMP0eracZLHUVp1Y5sqxKS9WXZir0VKUi3ppL7xH_iH_BLOThOoBg88RY6d2PGdfd_5LncA7yxi7JznLMSLCrm1WagTLUKjI2qYcFlFnEX37FxML_jJZXS58c2pO2_3ziTZ_tPgojSVzeEyt12-nuGhKfQ355cVp7EPuH0X1RTqdK-RGP328Ei8vuWPWDhN0k1kn7-8AAXL3PlB3gaZt30le4PpNpz18mjyCB5ugCQ5ain_GO6Ycg_utakl13vwYNRlcnsC1fjLz-8_loiTC0UKHBPKJ0PcF6usIc58XPuSz31lSFMRtXLxBtRVVVeFIXpRXbktcVETvSbXbfZ6pCepLPGykMyO2YzU6xLRJLZ6CheT8dfRNNwkWgjniKZEqNLEGdgGGRcJZ7lSnHFLteY8tTxVseURKjpUcxT3lGsbq4i1BlCrhGWMPYOdsirNcyBxPsgiHQk-UAlXmdBZNmSpUVQnaRQzFcB7nGm5bENpSBfcenp0KhclLrhCIjYZpiKmNzSAg44WcrOqaom6ZYIES2IewNu-GifTGTlw5qqVa4PbFGKUKApgvyVd39vQRW_DLgKIt4i6NZztmnIx9zG38UGnGgbwoSP_H8NCPcoxlXRMJXumCoB5_ujf_o92cnz28aQvvfiPHt7A_dmniTw9Pv_8EnbdbXfqTPkB7DTXK_MK4VKjX_tF8Qsxsw44
openUrl ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=ER%E2%80%93plasma+membrane+contact+sites+contribute+to+autophagosome+biogenesis+by+regulation+of+local+PI3P+synthesis&rft.jtitle=The+EMBO+journal&rft.au=Nascimbeni%2C+Anna+Chiara&rft.au=Giordano%2C+Francesca&rft.au=Dupont%2C+Nicolas&rft.au=Grasso%2C+Daniel&rft.date=2017-07-14&rft.issn=0261-4189&rft.eissn=1460-2075&rft.volume=36&rft.issue=14&rft.spage=2018&rft.epage=2033&rft_id=info:doi/10.15252%2Fembj.201797006&rft.externalDBID=10.15252%252Fembj.201797006&rft.externalDocID=EMBJ201797006
thumbnail_l http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=0261-4189&client=summon
thumbnail_m http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=0261-4189&client=summon
thumbnail_s http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=0261-4189&client=summon