Identification of a novel microRNA (miRNA) from rice that targets an alternatively spliced transcript of the Nramp6 (Natural resistance-associated macrophage protein 6) gene involved in pathogen resistance

Plants have evolved efficient defence mechanisms to defend themselves from pathogen attack. Although many studies have focused on the transcriptional regulation of defence responses, less is known about the involvement of microRNAs (miRNAs) as post-transcriptional regulators of gene expression in pl...

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Published inThe New phytologist Vol. 199; no. 1; pp. 212 - 227
Main Authors Campo, Sonia, Peris-Peris, Cristina, Siré, Christelle, Moreno, Ana Beatriz, Donaire, Livia, Zytnicki, Matthias, Notredame, Cedric, Llave, César, San Segundo, Blanca
Format Journal Article
LanguageEnglish
Published England New Phytologist Trust 01.07.2013
Wiley Subscription Services, Inc
Wiley
Subjects
Alternative Splicing
Blasts
Defence mechanisms
Disease resistance
DNA microarrays
Domestication
elicitors
Evolution
Fungi
Gene expression
Gene Expression Regulation, Plant
Gene regulation
Genomes
Genomics
Identification
Immunity
Life Sciences
Macrophages
Magnaporthe oryzae
Medical treatment
metal transporter
MicroRNA
MicroRNAs
MicroRNAs - genetics
MicroRNAs - metabolism
miRNA
mRNA processing
Nicotiana - genetics
Nramp
Nucleotide sequences
Oryza
Oryza - genetics
Oryza - microbiology
Oryza sativa
Pathogens
Plant Diseases - genetics
Plant Diseases - microbiology
Plant immunity
Plant Leaves - genetics
Plant Proteins - genetics
Plant Proteins - metabolism
Plant Roots - genetics
Plants
Plants, Genetically Modified
Proteins
Reproducibility of Results
Reverse transcriptase polymerase chain reaction
Ribonucleic acid
Rice
rice (Oryza sativa)
Rice blast
RNA
RNA, Plant - genetics
Species Specificity
Transcription
Vegetal Biology
Nramp
Alternative splicing
Elicitors
Magnaporthe oryzae
microRNA
Evolution
Metal transporter
Rice (oryza sativa)
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Abstract Plants have evolved efficient defence mechanisms to defend themselves from pathogen attack. Although many studies have focused on the transcriptional regulation of defence responses, less is known about the involvement of microRNAs (miRNAs) as post-transcriptional regulators of gene expression in plant immunity. This work investigates miRNAs that are regulated by elicitors from the blast fungus Magnaporthe oryzae in rice (Oryza sativa). Small RNA libraries were constructed from rice tissues and subjected to high-throughput sequencing for the identification of elicitor-responsive miRNAs. Target gene expression was examined by microarray analysis. Transgenic lines were used for the analysis of miRNA functioning in disease resistance. Elicitor treatment is accompanied by dynamic alterations in the expression of a significant number of miRNAs, including new members of annotated miRNAs. Novel miRNAs from rice are proposed. We report a new rice miRNA, osa-miR7695, which negatively regulates an alternatively spliced transcript of OsNramp6 (Natural resistance-associated macrophage protein 6). This novel miRNA experienced natural and domestication selection events during evolution, and its overexpression in rice confers pathogen resistance. This study highlights an miRNA-mediated regulation of OsNramp6 in disease resistance, whilst illustrating the existence of a novel regulatory network that integrates miRNA function and mRNA processing in plant immunity.
AbstractList Plants have evolved efficient defence mechanisms to defend themselves from pathogen attack. Although many studies have focused on the transcriptional regulation of defence responses, less is known about the involvement of microRNAs (miRNAs) as post-transcriptional regulators of gene expression in plant immunity. This work investigates miRNAs that are regulated by elicitors from the blast fungus Magnaporthe oryzae in rice (Oryza sativa). Small RNA libraries were constructed from rice tissues and subjected to high-throughput sequencing for the identification of elicitor-responsive miRNAs. Target gene expression was examined by microarray analysis. Transgenic lines were used for the analysis of miRNA functioning in disease resistance. Elicitor treatment is accompanied by dynamic alterations in the expression of a significant number of miRNAs, including new members of annotated miRNAs. Novel miRNAs from rice are proposed. We report a new rice miRNA, osa-miR7695, which negatively regulates an alternatively spliced transcript of OsNramp6 (Natural resistance-associated macrophage protein 6). This novel miRNA experienced natural and domestication selection events during evolution, and its overexpression in rice confers pathogen resistance. This study highlights an miRNA-mediated regulation of OsNramp6 in disease resistance, whilst illustrating the existence of a novel regulatory network that integrates miRNA function and mRNA processing in plant immunity.
Summary Plants have evolved efficient defence mechanisms to defend themselves from pathogen attack. Although many studies have focused on the transcriptional regulation of defence responses, less is known about the involvement of microRNAs (miRNAs) as post‐transcriptional regulators of gene expression in plant immunity. This work investigates miRNAs that are regulated by elicitors from the blast fungus Magnaporthe oryzae in rice (Oryza sativa). Small RNA libraries were constructed from rice tissues and subjected to high‐throughput sequencing for the identification of elicitor‐responsive miRNAs. Target gene expression was examined by microarray analysis. Transgenic lines were used for the analysis of miRNA functioning in disease resistance. Elicitor treatment is accompanied by dynamic alterations in the expression of a significant number of miRNAs, including new members of annotated miRNAs. Novel miRNAs from rice are proposed. We report a new rice miRNA, osa‐miR7695, which negatively regulates an alternatively spliced transcript of OsNramp6 (Natural resistance‐associated macrophage protein 6). This novel miRNA experienced natural and domestication selection events during evolution, and its overexpression in rice confers pathogen resistance. This study highlights an miRNA‐mediated regulation of OsNramp6 in disease resistance, whilst illustrating the existence of a novel regulatory network that integrates miRNA function and mRNA processing in plant immunity.
Plants have evolved efficient defence mechanisms to defend themselves from pathogen attack. Although many studies have focused on the transcriptional regulation of defence responses, less is known about the involvement of microRNAs (miRNAs) as post-transcriptional regulators of gene expression in plant immunity. This work investigates miRNAs that are regulated by elicitors from the blast fungus Magnaporthe oryzae in rice (Oryza sativa). Small RNA libraries were constructed from rice tissues and subjected to high-throughput sequencing for the identification of elicitor-responsive miRNAs. Target gene expression was examined by microarray analysis. Transgenic lines were used for the analysis of miRNA functioning in disease resistance. Elicitor treatment is accompanied by dynamic alterations in the expression of a significant number of miRNAs, including new members of annotated miRNAs. Novel miRNAs from rice are proposed. We report a new rice miRNA, osa-miR7695, which negatively regulates an alternatively spliced transcript of OsNramp6 (Natural resistance-associated macrophage protein 6). This novel miRNA experienced natural and domestication selection events during evolution, and its overexpression in rice confers pathogen resistance. This study highlights an miRNA-mediated regulation of OsNramp6 in disease resistance, whilst illustrating the existence of a novel regulatory network that integrates miRNA function and mRNA processing in plant immunity.
Summary Plants have evolved efficient defence mechanisms to defend themselves from pathogen attack. Although many studies have focused on the transcriptional regulation of defence responses, less is known about the involvement of microRNAs (miRNAs) as post-transcriptional regulators of gene expression in plant immunity. This work investigates miRNAs that are regulated by elicitors from the blast fungus Magnaporthe oryzae in rice (Oryza sativa). Small RNA libraries were constructed from rice tissues and subjected to high-throughput sequencing for the identification of elicitor-responsive miRNAs. Target gene expression was examined by microarray analysis. Transgenic lines were used for the analysis of miRNA functioning in disease resistance. Elicitor treatment is accompanied by dynamic alterations in the expression of a significant number of miRNAs, including new members of annotated miRNAs. Novel miRNAs from rice are proposed. We report a new rice miRNA, osa-miR7695, which negatively regulates an alternatively spliced transcript of OsNramp6 (Natural resistance-associated macrophage protein 6). This novel miRNA experienced natural and domestication selection events during evolution, and its overexpression in rice confers pathogen resistance. This study highlights an miRNA-mediated regulation of OsNramp6 in disease resistance, whilst illustrating the existence of a novel regulatory network that integrates miRNA function and mRNA processing in plant immunity. [PUBLICATION ABSTRACT]
Author Cristina Peris-Peris
Livia Donaire
Christelle Siré
Blanca San Segundo
Ana Beatriz Moreno
Matthias Zytnicki
César Llave
Sonia Campo
Cedric Notredame
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Issue 1
Keywords Nramp
Alternative splicing
Elicitors
Magnaporthe oryzae
microRNA
Evolution
Metal transporter
Rice (oryza sativa)
Language English
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Snippet Plants have evolved efficient defence mechanisms to defend themselves from pathogen attack. Although many studies have focused on the transcriptional...
Summary Plants have evolved efficient defence mechanisms to defend themselves from pathogen attack. Although many studies have focused on the transcriptional...
Summary Plants have evolved efficient defence mechanisms to defend themselves from pathogen attack. Although many studies have focused on the transcriptional...
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wiley
jstor
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StartPage 212
SubjectTerms Alternative Splicing
Blasts
Defence mechanisms
Disease resistance
DNA microarrays
Domestication
elicitors
Evolution
Fungi
Gene expression
Gene Expression Regulation, Plant
Gene regulation
Genomes
Genomics
Identification
Immunity
Life Sciences
Macrophages
Magnaporthe oryzae
Medical treatment
metal transporter
MicroRNA
MicroRNAs
MicroRNAs - genetics
MicroRNAs - metabolism
miRNA
mRNA processing
Nicotiana - genetics
Nramp
Nucleotide sequences
Oryza
Oryza - genetics
Oryza - microbiology
Oryza sativa
Pathogens
Plant Diseases - genetics
Plant Diseases - microbiology
Plant immunity
Plant Leaves - genetics
Plant Proteins - genetics
Plant Proteins - metabolism
Plant Roots - genetics
Plants
Plants, Genetically Modified
Proteins
Reproducibility of Results
Reverse transcriptase polymerase chain reaction
Ribonucleic acid
Rice
rice (Oryza sativa)
Rice blast
RNA
RNA, Plant - genetics
Species Specificity
Transcription
Vegetal Biology
Title Identification of a novel microRNA (miRNA) from rice that targets an alternatively spliced transcript of the Nramp6 (Natural resistance-associated macrophage protein 6) gene involved in pathogen resistance
URI https://www.jstor.org/stable/newphytologist.199.1.212
https://onlinelibrary.wiley.com/doi/abs/10.1111%2Fnph.12292
https://www.ncbi.nlm.nih.gov/pubmed/23627500
https://www.proquest.com/docview/1432320253
https://www.proquest.com/docview/2513380620
https://search.proquest.com/docview/1356955345
https://search.proquest.com/docview/1399906589
https://hal.inrae.fr/hal-02650361
Volume 199
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