Mimicking natural polymorphism in eIF4E by CRISPR‐Cas9 base editing is associated with resistance to potyviruses

• Published in: Plant biotechnology journal Vol. 17; no. 9; pp. 1736 - 1750
• Main Authors: Bastet, Anna, Zafirov, Delyan, Giovinazzo, Nathalie, Guyon‐Debast, Anouchka, Nogué, Fabien, Robaglia, Christophe, Gallois, Jean‐Luc
• Format: Journal Article
• Language: English
• Published: England Wiley 01.09.2019; John Wiley and Sons Inc
• Subjects: Alleles; amino acids; Arabidopsis - genetics; Arabidopsis - virology; Arabidopsis thaliana; base editing; biotechnology; Clover yellow vein virus; CRISPR-Cas Systems; CRISPR‐Cas9; crops; cytidine deaminase; Disease Resistance - genetics; eIF4E; Eukaryotic Initiation Factor-4E - genetics; Gene Editing; genetic resistance; Life Sciences; loss-of-function mutation; pathogens; phenotype; Pisum sativum; Pisum sativum - genetics; Pisum sativum - virology; Plant Diseases - genetics; Plant Diseases - virology; plant growth; Plant Proteins - genetics; Plants, Genetically Modified; potyvirus; Potyvirus - pathogenicity; viruses; Arabidopsis thaliana; potyvirus; eIF4E; cytidine deaminase; base editing; CRISPR-Cas9

Summary In many crop species, natural variation in eIF4E proteins confers resistance to potyviruses. Gene editing offers new opportunities to transfer genetic resistance to crops that seem to lack natural eIF4E alleles. However, because eIF4E are physiologically important proteins, any introduced mo...