Muscarinic Ca2+ responses resistant to muscarinic antagonists at perisynaptic Schwann cells of the frog neuromuscular junction

• Published in: The Journal of physiology Vol. 504; no. Pt 2; pp. 337 - 347
• Main Authors: Robitaille, Richard, Jahromi, Babak S., Charlton, Milton P.
• Format: Journal Article
• Language: English
• Published: Oxford, UK The Physiological Society 15.10.1997; Blackwell Science Ltd
• Subjects: Acetylcholine - pharmacology; Adenosine - pharmacology; Aniline Compounds - metabolism; Animals; Calcium - metabolism; Fluorescent Dyes - metabolism; Gallamine Triethiodide - pharmacology; Microscopy, Fluorescence; Muscarine - pharmacology; Muscarinic Agonists - pharmacology; Muscarinic Antagonists - pharmacology; Neuromuscular Junction - drug effects; Neuromuscular Junction - metabolism; Nicotine - pharmacology; Nicotinic Agonists - pharmacology; Nicotinic Antagonists - pharmacology; Pertussis Toxin; Rana pipiens; Receptors, Muscarinic - metabolism; Schwann Cells - cytology; Schwann Cells - drug effects; Schwann Cells - metabolism; Virulence Factors, Bordetella - pharmacology; Xanthenes - metabolism

1. Acetylcholine causes a rise of intracellular Ca2+ in perisynaptic Schwann cells (PSCs) of the frog neuromuscular junction. The signalling pathway was characterized using the fluorescent Ca2+ indicator fluo-3 and fluorescence microscopy. 2. Nicotinic antagonists had no effect on Ca2+ responses evo...