Ca2+-calmodulin promotes survival of pheromone-induced growth arrest by activation of calcineurin and Ca2+-calmodulin-dependent protein kinase
Article Usage Stats Services MCB Citing Articles Google Scholar PubMed Related Content Social Bookmarking CiteULike Delicious Digg Facebook Google+ Mendeley Reddit StumbleUpon Twitter current issue Spotlights in the Current Issue MCB About MCB Subscribers Authors Reviewers Advertisers Inquiries from...
Saved in:
Published in | Molecular and Cellular Biology Vol. 16; no. 9; pp. 4824 - 4831 |
---|---|
Main Authors | , , |
Format | Journal Article |
Language | English |
Published |
United States
American Society for Microbiology
01.09.1996
Taylor & Francis |
Subjects | |
Online Access | Get full text |
Cover
Loading…
Abstract | Article Usage Stats
Services
MCB
Citing Articles
Google Scholar
PubMed
Related Content
Social Bookmarking
CiteULike
Delicious
Digg
Facebook
Google+
Mendeley
Reddit
StumbleUpon
Twitter
current issue
Spotlights in the Current Issue
MCB
About
MCB
Subscribers
Authors
Reviewers
Advertisers
Inquiries from the Press
Permissions & Commercial Reprints
ASM Journals Public Access Policy
MCB
RSS Feeds
1752 N Street N.W. • Washington DC 20036
202.737.3600 • 202.942.9355 fax • journals@asmusa.org
Print ISSN:
0270-7306
Online ISSN:
1098-5549
Copyright © 2014
by the
American Society for Microbiology.
For an alternate route to
MCB
.asm.org, visit:
MCB
|
---|---|
AbstractList | Article Usage Stats
Services
MCB
Citing Articles
Google Scholar
PubMed
Related Content
Social Bookmarking
CiteULike
Delicious
Digg
Facebook
Google+
Mendeley
Reddit
StumbleUpon
Twitter
current issue
Spotlights in the Current Issue
MCB
About
MCB
Subscribers
Authors
Reviewers
Advertisers
Inquiries from the Press
Permissions & Commercial Reprints
ASM Journals Public Access Policy
MCB
RSS Feeds
1752 N Street N.W. • Washington DC 20036
202.737.3600 • 202.942.9355 fax • journals@asmusa.org
Print ISSN:
0270-7306
Online ISSN:
1098-5549
Copyright © 2014
by the
American Society for Microbiology.
For an alternate route to
MCB
.asm.org, visit:
MCB
The cmd1-6 allele contains three mutations that block Ca 2+ binding to calmodulin from Saccharomyces cerevisiae. We find that strains containing cmd1-6 lose viability during cell cycle arrest induced by the mating pheromone α-factor. The 50% lethal dose (LD 50 )of α-factor for the calmodulin mutant is almost fivefold below the LD 50 for a wild-type strain. The calmodulin mutants are not more sensitive to α-factor, as measured by activation of a pheromone-responsive reporter gene. Two observations indicate that activation of the Ca 2+ -calmodulin-dependent protein phosphatase calcineurin contributes to survival of pheromone-induced arrest. First, deletion of the gene encoding the calcineurin regulatory B subunit, CNB1, from a wild-type strain decreases the LD 50 of α-factor but has no further effect on a cmd1-6 strain. Second, a dominant constitutive calcineurin mutant partially restores the ability of the cmd1-6 strain to survive exposure to α-factor. Activation of the Ca 2+ -calmodulin-dependent protein kinase (CaMK) also contributes to survival, thus revealing a new function for this enzyme. Deletion of the CMK1 and CMK2 genes, which encode CaMK, decreases the LD 50 of pheromone compared with that for a wild-type strain but again has no effect in a cmd1-6 strain. Furthermore, the LD 50 of α-factor for a mutant in which the calcineurin and CaMK genes have been deleted is the same as that for the calmodulin mutant. Finally, the CaMK and calcineurin pathways appear to be independent since the ability of constitutive calcineurin to rescue a cmd1-6 strain is not blocked by deletion of the CaMK genes. The cmd1-6 allele contains three mutations that block Ca2+ binding to calmodulin from Saccharomyces cerevisiae. We find that strains containing cmd1-6 lose viability during cell cycle arrest induced by the mating pheromone alpha-factor. The 50% lethal dose (LD50) of alpha-factor for the calmodulin mutant is almost fivefold below the LD50 for a wild-type strain. The calmodulin mutants are not more sensitive to alpha-factor, as measured by activation of a pheromone-responsive reporter gene. Two observations indicate that activation of the Ca2+-calmodulin-dependent protein phosphatase calcineurin contributes to survival of pheromone-induced arrest. First, deletion of the gene encoding the calcineurin regulatory B subunit, CNB1, from a wild-type strain decreases the LD50 of alpha-factor but has no further effect on a cmd1-6 strain. Second, a dominant constitutive calcineurin mutant partially restores the ability of the cmd1-6 strain to survive exposure to alpha-factor. Activation of the Ca2+-calmodulin-dependent protein kinase (CaMK) also contributes to survival, thus revealing a new function for this enzyme. Deletion of the CMK1 and CMK2 genes, which encode CaMK, decreases the LD50 of pheromone compared with that for a wild-type strain but again has no effect in a cmd1-6 strain. Furthermore, the LD50 of alpha-factor for a mutant in which the calcineurin and CaMK genes have been deleted is the same as that for the calmodulin mutant. Finally, the CaMK and calcineurin pathways appear to be independent since the ability of constitutive calcineurin to rescue a cmd1-6 strain is not blocked by deletion of the CaMK genes. |
Author | J R Geiser M J Moser T N Davis |
AuthorAffiliation | Department of Biochemistry, University of Washington, Seattle 98195, USA |
AuthorAffiliation_xml | – name: Department of Biochemistry, University of Washington, Seattle 98195, USA |
Author_xml | – sequence: 1 givenname: Michael J. surname: Moser fullname: Moser, Michael J. organization: Department of Biochemistry, University of Washington – sequence: 2 givenname: John R. surname: Geiser fullname: Geiser, John R. organization: Department of Biochemistry, University of Washington – sequence: 3 givenname: Trisha N. surname: Davis fullname: Davis, Trisha N. organization: Department of Biochemistry, University of Washington |
BackLink | https://www.ncbi.nlm.nih.gov/pubmed/8756641$$D View this record in MEDLINE/PubMed |
BookMark | eNpdkUtv1DAUhS3UqkwLS5ZIZo0y9StOsmBBRy0gFbGBtXXjx8SQ2JGTzGj-BL8ZR1OhwsqSz_nOvbrnGl2EGCxCbyjZUsrq26-7uy2V22YraiZeoA0lTV2UpWgu0IawihQVJ_Ilup6mn4QQ2RB-ha7qqpRS0A36vQP2vtDQD9EsvQ94THGIs53wtKSDP0CPo8NjZ9fvYAsfzKKtwfsUj3OHISU7zbg9YdBzds8-hhXIgdoHu6ScCMHg_6YUxo42GBvmdd5ss-uXDzDZV-jSQT_Z10_vDfrxcP9997l4_Pbpy-7jY9Hxis0FE1bzmrbMaedE5TQviTZt2eSLWN0Iagw4qaGRHEBXdcsJ55IxS4AxWQG_QR_OuePSDtbovEmCXo3JD5BOKoJX_yrBd2ofD4pxKmqR-bfP-b_g012zXp91H1xMAxxj6o2a4dTH5BIE7SfFKVFrgyo3qKhUjVobzOi7M9r5fXf0ySqYBjXo9pnnD7jAnsw |
ContentType | Journal Article |
Copyright | Copyright © 1996, American Society for Microbiology 1996 |
Copyright_xml | – notice: Copyright © 1996, American Society for Microbiology 1996 |
DBID | CGR CUY CVF ECM EIF NPM 5PM |
DOI | 10.1128/MCB.16.9.4824 |
DatabaseName | Medline MEDLINE MEDLINE (Ovid) MEDLINE MEDLINE PubMed PubMed Central (Full Participant titles) |
DatabaseTitle | MEDLINE Medline Complete MEDLINE with Full Text PubMed MEDLINE (Ovid) |
DatabaseTitleList | MEDLINE |
Database_xml | – sequence: 1 dbid: NPM name: PubMed url: https://proxy.k.utb.cz/login?url=http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed sourceTypes: Index Database – sequence: 2 dbid: EIF name: MEDLINE url: https://proxy.k.utb.cz/login?url=https://www.webofscience.com/wos/medline/basic-search sourceTypes: Index Database |
DeliveryMethod | fulltext_linktorsrc |
Discipline | Chemistry Biology |
EISSN | 1098-5549 |
EndPage | 4831 |
ExternalDocumentID | 8756641 12263552 mcb_16_9_4824 |
Genre | Research Article Research Support, U.S. Gov't, P.H.S Journal Article |
GrantInformation_xml | – fundername: NIGMS NIH HHS grantid: GM40506 |
GroupedDBID | --- -DZ -~X .55 .GJ 08R 0R~ 123 18M 29M 2WC 39C 3O- 4.4 53G 5RE 5VS 9M8 AAPBV AAUGY ABPTK ACGFO ACKIV ACNCT ADBBV ADIYS AENEX AFFNX AGVNZ ALMA_UNASSIGNED_HOLDINGS AOIJS BAWUL BTFSW C1A CS3 DIK DU5 E3Z EBS EJD F20 F5P GX1 H13 HH5 HYE HZ~ IH2 KQ8 L7B MVM N9A O9- OK1 P2P RHF RHI RNS RPM RSF TFL TFW TR2 UCJ UDS VQA W8F WH7 WOQ X7M Y6R YYP ZA5 ZCA ZGI ZXP ABJNI ABRLO ABTAH AEOZL AGHSJ CGR CUY CVF ECM EIF EMOBN M4Z NPM TDBHL WHG ZY4 5PM |
ID | FETCH-LOGICAL-h372t-24ec381b2fcff47fc350cdb59112ec941ddaf6ca963aac78b3033622e0a2267a3 |
IEDL.DBID | RPM |
ISSN | 0270-7306 |
IngestDate | Tue Sep 17 21:34:51 EDT 2024 Sat Sep 28 08:40:05 EDT 2024 Tue Jun 13 19:24:40 EDT 2023 Wed May 18 15:28:12 EDT 2016 |
IsDoiOpenAccess | false |
IsOpenAccess | true |
IsPeerReviewed | true |
IsScholarly | true |
Issue | 9 |
Language | English |
LinkModel | DirectLink |
MergedId | FETCHMERGED-LOGICAL-h372t-24ec381b2fcff47fc350cdb59112ec941ddaf6ca963aac78b3033622e0a2267a3 |
OpenAccessLink | https://mcb.asm.org/content/mcb/16/9/4824.full.pdf |
PMID | 8756641 |
PageCount | 8 |
ParticipantIDs | pubmed_primary_8756641 informaworld_taylorfrancis_310_1128_MCB_16_9_4824 pubmedcentral_primary_oai_pubmedcentral_nih_gov_231484 highwire_asm_mcb_16_9_4824 |
PublicationCentury | 1900 |
PublicationDate | 19960901 9/1/1996 1996-Sep |
PublicationDateYYYYMMDD | 1996-09-01 |
PublicationDate_xml | – month: 09 year: 1996 text: 19960901 day: 01 |
PublicationDecade | 1990 |
PublicationPlace | United States |
PublicationPlace_xml | – name: United States |
PublicationTitle | Molecular and Cellular Biology |
PublicationTitleAlternate | Mol Cell Biol |
PublicationYear | 1996 |
Publisher | American Society for Microbiology Taylor & Francis |
Publisher_xml | – name: American Society for Microbiology – name: Taylor & Francis |
SSID | ssj0006903 |
Score | 1.8796599 |
Snippet | Article Usage Stats
Services
MCB
Citing Articles
Google Scholar
PubMed
Related Content
Social Bookmarking
CiteULike
Delicious
Digg
Facebook
Google+
Mendeley... The cmd1-6 allele contains three mutations that block Ca 2+ binding to calmodulin from Saccharomyces cerevisiae. We find that strains containing cmd1-6 lose... The cmd1-6 allele contains three mutations that block Ca2+ binding to calmodulin from Saccharomyces cerevisiae. We find that strains containing cmd1-6 lose... |
SourceID | pubmedcentral pubmed informaworld highwire |
SourceType | Open Access Repository Index Database Publisher |
StartPage | 4824 |
SubjectTerms | Base Sequence Calcineurin Calcium - physiology Calcium-Calmodulin-Dependent Protein Kinases - genetics Calcium-Calmodulin-Dependent Protein Kinases - physiology Calmodulin - genetics Calmodulin - physiology Calmodulin-Binding Proteins - physiology Enzyme Activation Fungal Proteins - physiology Mating Factor Molecular Sequence Data Peptides - physiology Phosphoprotein Phosphatases - physiology Saccharomyces cerevisiae - drug effects Saccharomyces cerevisiae - physiology Sequence Deletion |
Title | Ca2+-calmodulin promotes survival of pheromone-induced growth arrest by activation of calcineurin and Ca2+-calmodulin-dependent protein kinase |
URI | http://mcb.asm.org/content/16/9/4824.abstract https://www.tandfonline.com/doi/abs/10.1128/MCB.16.9.4824 https://www.ncbi.nlm.nih.gov/pubmed/8756641 https://pubmed.ncbi.nlm.nih.gov/PMC231484 |
Volume | 16 |
hasFullText | 1 |
inHoldings | 1 |
isFullTextHit | |
isPrint | |
link | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV1Lb9QwEB6xlSp6QVCo2EIrH7ghZxPHcZwjRFQVVSuQqNSb5fjBrmjSFZse-if4zYydpNrtsdc4dizPxPPw528APgXKsgpNN80MwwDFC0u1YwW1mWdaaqOljSjfK3F-zb_fFDfjpbDNCKvsTLNKuts26VbLiK1ct2Yx4cQWPy5r9Em45IsZzMo8nyL0cffFaC-eKrMypai9YuLVZHJxWX9NMpFUCZeMH8A-uupChFLwj9TAT_hKt6zSLmJyywSdvYZXo-9IvgxzfAMvXHcI-0M1yYdDeFlPxdvewr9as88U17-9swFrTtYRduc2ZHOPmwOqF7nzJFAK4OPOUYzMUcaW_MaovF8SHUt2kOaBhHsPQ9Y2dMABw0F8yNAT3Vny5Ct0Kqnbk8j_gG_9WXVoJ9_B9dm3X_U5HUsv0GVesp4y7gza8oZ54z0vvcmL1NimwK2ROVPxzFrthdH4-2ptStmgJURTyFyq0Z8rdX4Eex1O_z0QWTmbF6UuMud4ikO4kHfKM9boxtmUz-F4Wn6lN61qTaMyoSoVpDSHbFsgqo9pDD_UHFF5jGCYVCjZ7T5Hg7DUeiDsUKOg5yB2pPjYHii2d1tQ8yLV9qBpx8_t-AEOBpR3wKR9hL3-7707QSemb05hdvFTnkbd_Q_FMvar |
link.rule.ids | 230,315,733,786,790,891,27955,27956,53825,53827 |
linkProvider | National Library of Medicine |
linkToHtml | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV1Lb9QwEB5BUdVeKBQqtlDwgRtyNnGc1xGiVgt0Kw5dqTfLr7CrknTFZg_lR_CbGTtJtbu3co1jx9GMPQ9__gbgo6MsK9B000gzDFCq1FBpWUJNVDGZSy1z41G-V-lkxr_dJDf9pbBVD6tstFoEza86aBZzj61c1no84MTGP6Yl-iQ85-On8AyXK0uGGL3ffzHe8-fKLAsp6m86MGuyfDwtvwRRGhQBzxk_hH101tPUFYN_IAfeYSzdsEvbmMkNI3RxBLNh-h325DZYtyrQf3aYHR_7fy_gee-Vks9d60t4Yptj2O_qVN4fw0E5lIV7BX9LyT5RlGx9ZxyKnSw9oM-uyGqN2w4qLrmriCMrwMeNpRjzo_YY8hPj_XZOpC8GQtQ9cTcqunyw64ADuiN-l_snsjFk5yt0KNbbEs8sgW_dLhq0wK9hdnF-XU5oX9SBzuOMtZRxq9FLUKzSVcWzSsdJqI1KcNNlVhc8MkZWqZa4MUips1yhjUUjy2wo0VPMZHwCew1O_w2QvLAmTjKZRNbyEIewLqMVR0xJZU3IR3A6iFXIVS1qrUSUikI46Y8g2hS0aH2CpOqqmYjYx0YsF6gxm31OOiUQy44KRPQKNIJ0Szse2h1593YLytyTeHcyPv3fjh_gYHI9vRSXX6--v4XDDkvukG_vYK_9vbZn6Cq16r1fGf8Aly4XgA |
linkToPdf | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV3JbtswEB2kKdLm0iVtEKcbD70VlCWK2o6tWiNdEuTQAOmJ4FobqRQjlg_pR_SbO6SswPYxV1GkRMxwFvLxDcB7T1lWoeumiWaYoLjcUGlZRk3imCyllqUJKN-z_OSCf7vMLnegHO7CBNC-VrOo_dNE7WwasJXzRo8HnNj4_LTGmISXfDw3bvwAHuKSZcWQp69sMOZ84WyZFTFFHc4Hdk1Wjk_rT1GSR1XES8b3YQ8D9jz3BeHvCIK3WEvXfNMmbnLNEU2ewq9hCj3-5CpadirSf7fYHe8zx2fwZBWdko_9G89hx7YHsNfXq7w9gMf1UB7uBfyrJftAUcLNtfFodjIPwD67IIslmh9UYHLtiCctwMetpZj7oxYZ8hvz_m5KZCgKQtQt8Tcr-n1h3wEH9Ef9_gyAyNaQra_QoWhvRwLDBL51NWvRE7-Ei8mXn_UJXRV3oNO0YB1l3GqMFhRz2jleOJ1msTYqQ-PLrK54Yox0uZZoIKTURanQ16KzZTaWGDEWMj2E3RZ__whIWVmTZoXMEmt5jENYv7OVJkxJZU3MR3A8iFbIRSMarUSSi0p4DRhBsi5s0YWNEtdXNRFpyJFYKVBr1vsc9oog5j0liFgp0QjyDQ25a_ck3pstKPdA5t3L-fi-Hd_Bo_PPE_Hj69n3V7DfQ8o9AO417HY3S_sGI6ZOvQ2L4z8zrxoA |
openUrl | ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Ca2%2B-calmodulin+promotes+survival+of+pheromone-induced+growth+arrest+by+activation+of+calcineurin+and+Ca2%2B-calmodulin-dependent+protein+kinase&rft.jtitle=Molecular+and+Cellular+Biology&rft.au=M+J+Moser&rft.au=J+R+Geiser&rft.au=T+N+Davis&rft.date=1996-09-01&rft.pub=American+Society+for+Microbiology&rft.issn=0270-7306&rft.eissn=1098-5549&rft.volume=16&rft.issue=9&rft.spage=4824&rft_id=info:doi/10.1128%2FMCB.16.9.4824&rft_id=info%3Apmid%2F8756641&rft.externalDBID=n%2Fa&rft.externalDocID=mcb_16_9_4824 |
thumbnail_l | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=0270-7306&client=summon |
thumbnail_m | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=0270-7306&client=summon |
thumbnail_s | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=0270-7306&client=summon |