Ca2+-calmodulin promotes survival of pheromone-induced growth arrest by activation of calcineurin and Ca2+-calmodulin-dependent protein kinase

Article Usage Stats Services MCB Citing Articles Google Scholar PubMed Related Content Social Bookmarking CiteULike Delicious Digg Facebook Google+ Mendeley Reddit StumbleUpon Twitter current issue Spotlights in the Current Issue MCB About MCB Subscribers Authors Reviewers Advertisers Inquiries from...

Full description

Saved in:
Bibliographic Details
Published inMolecular and Cellular Biology Vol. 16; no. 9; pp. 4824 - 4831
Main Authors Moser, Michael J., Geiser, John R., Davis, Trisha N.
Format Journal Article
LanguageEnglish
Published United States American Society for Microbiology 01.09.1996
Taylor & Francis
Subjects
Base Sequence
Calcineurin
Calcium - physiology
Calcium-Calmodulin-Dependent Protein Kinases - genetics
Calcium-Calmodulin-Dependent Protein Kinases - physiology
Calmodulin - genetics
Calmodulin - physiology
Calmodulin-Binding Proteins - physiology
Enzyme Activation
Fungal Proteins - physiology
Mating Factor
Molecular Sequence Data
Peptides - physiology
Phosphoprotein Phosphatases - physiology
Saccharomyces cerevisiae - drug effects
Saccharomyces cerevisiae - physiology
Sequence Deletion
Online AccessGet full text

Cover

Abstract Article Usage Stats Services MCB Citing Articles Google Scholar PubMed Related Content Social Bookmarking CiteULike Delicious Digg Facebook Google+ Mendeley Reddit StumbleUpon Twitter current issue Spotlights in the Current Issue MCB About MCB Subscribers Authors Reviewers Advertisers Inquiries from the Press Permissions & Commercial Reprints ASM Journals Public Access Policy MCB RSS Feeds 1752 N Street N.W. • Washington DC 20036 202.737.3600 • 202.942.9355 fax • journals@asmusa.org Print ISSN: 0270-7306 Online ISSN: 1098-5549 Copyright © 2014 by the American Society for Microbiology.   For an alternate route to MCB .asm.org, visit: MCB       
AbstractList Article Usage Stats Services MCB Citing Articles Google Scholar PubMed Related Content Social Bookmarking CiteULike Delicious Digg Facebook Google+ Mendeley Reddit StumbleUpon Twitter current issue Spotlights in the Current Issue MCB About MCB Subscribers Authors Reviewers Advertisers Inquiries from the Press Permissions & Commercial Reprints ASM Journals Public Access Policy MCB RSS Feeds 1752 N Street N.W. • Washington DC 20036 202.737.3600 • 202.942.9355 fax • journals@asmusa.org Print ISSN: 0270-7306 Online ISSN: 1098-5549 Copyright © 2014 by the American Society for Microbiology.   For an alternate route to MCB .asm.org, visit: MCB       
The cmd1-6 allele contains three mutations that block Ca 2+ binding to calmodulin from Saccharomyces cerevisiae. We find that strains containing cmd1-6 lose viability during cell cycle arrest induced by the mating pheromone α-factor. The 50% lethal dose (LD 50 )of α-factor for the calmodulin mutant is almost fivefold below the LD 50 for a wild-type strain. The calmodulin mutants are not more sensitive to α-factor, as measured by activation of a pheromone-responsive reporter gene. Two observations indicate that activation of the Ca 2+ -calmodulin-dependent protein phosphatase calcineurin contributes to survival of pheromone-induced arrest. First, deletion of the gene encoding the calcineurin regulatory B subunit, CNB1, from a wild-type strain decreases the LD 50 of α-factor but has no further effect on a cmd1-6 strain. Second, a dominant constitutive calcineurin mutant partially restores the ability of the cmd1-6 strain to survive exposure to α-factor. Activation of the Ca 2+ -calmodulin-dependent protein kinase (CaMK) also contributes to survival, thus revealing a new function for this enzyme. Deletion of the CMK1 and CMK2 genes, which encode CaMK, decreases the LD 50 of pheromone compared with that for a wild-type strain but again has no effect in a cmd1-6 strain. Furthermore, the LD 50 of α-factor for a mutant in which the calcineurin and CaMK genes have been deleted is the same as that for the calmodulin mutant. Finally, the CaMK and calcineurin pathways appear to be independent since the ability of constitutive calcineurin to rescue a cmd1-6 strain is not blocked by deletion of the CaMK genes.
The cmd1-6 allele contains three mutations that block Ca2+ binding to calmodulin from Saccharomyces cerevisiae. We find that strains containing cmd1-6 lose viability during cell cycle arrest induced by the mating pheromone alpha-factor. The 50% lethal dose (LD50) of alpha-factor for the calmodulin mutant is almost fivefold below the LD50 for a wild-type strain. The calmodulin mutants are not more sensitive to alpha-factor, as measured by activation of a pheromone-responsive reporter gene. Two observations indicate that activation of the Ca2+-calmodulin-dependent protein phosphatase calcineurin contributes to survival of pheromone-induced arrest. First, deletion of the gene encoding the calcineurin regulatory B subunit, CNB1, from a wild-type strain decreases the LD50 of alpha-factor but has no further effect on a cmd1-6 strain. Second, a dominant constitutive calcineurin mutant partially restores the ability of the cmd1-6 strain to survive exposure to alpha-factor. Activation of the Ca2+-calmodulin-dependent protein kinase (CaMK) also contributes to survival, thus revealing a new function for this enzyme. Deletion of the CMK1 and CMK2 genes, which encode CaMK, decreases the LD50 of pheromone compared with that for a wild-type strain but again has no effect in a cmd1-6 strain. Furthermore, the LD50 of alpha-factor for a mutant in which the calcineurin and CaMK genes have been deleted is the same as that for the calmodulin mutant. Finally, the CaMK and calcineurin pathways appear to be independent since the ability of constitutive calcineurin to rescue a cmd1-6 strain is not blocked by deletion of the CaMK genes.
Author J R Geiser
M J Moser
T N Davis
AuthorAffiliation Department of Biochemistry, University of Washington, Seattle 98195, USA
AuthorAffiliation_xml – name: Department of Biochemistry, University of Washington, Seattle 98195, USA
Author_xml – sequence: 1
  givenname: Michael J.
  surname: Moser
  fullname: Moser, Michael J.
  organization: Department of Biochemistry, University of Washington
– sequence: 2
  givenname: John R.
  surname: Geiser
  fullname: Geiser, John R.
  organization: Department of Biochemistry, University of Washington
– sequence: 3
  givenname: Trisha N.
  surname: Davis
  fullname: Davis, Trisha N.
  organization: Department of Biochemistry, University of Washington
BackLink https://www.ncbi.nlm.nih.gov/pubmed/8756641$$D View this record in MEDLINE/PubMed
BookMark eNpdkUtv1DAUhS3UqkwLS5ZIZo0y9StOsmBBRy0gFbGBtXXjx8SQ2JGTzGj-BL8ZR1OhwsqSz_nOvbrnGl2EGCxCbyjZUsrq26-7uy2V22YraiZeoA0lTV2UpWgu0IawihQVJ_Ilup6mn4QQ2RB-ha7qqpRS0A36vQP2vtDQD9EsvQ94THGIs53wtKSDP0CPo8NjZ9fvYAsfzKKtwfsUj3OHISU7zbg9YdBzds8-hhXIgdoHu6ScCMHg_6YUxo42GBvmdd5ss-uXDzDZV-jSQT_Z10_vDfrxcP9997l4_Pbpy-7jY9Hxis0FE1bzmrbMaedE5TQviTZt2eSLWN0Iagw4qaGRHEBXdcsJ55IxS4AxWQG_QR_OuePSDtbovEmCXo3JD5BOKoJX_yrBd2ofD4pxKmqR-bfP-b_g012zXp91H1xMAxxj6o2a4dTH5BIE7SfFKVFrgyo3qKhUjVobzOi7M9r5fXf0ySqYBjXo9pnnD7jAnsw
ContentType Journal Article
Copyright Copyright © 1996, American Society for Microbiology 1996
Copyright_xml – notice: Copyright © 1996, American Society for Microbiology 1996
DBID CGR
CUY
CVF
ECM
EIF
NPM
5PM
DOI 10.1128/MCB.16.9.4824
DatabaseName Medline
MEDLINE
MEDLINE (Ovid)
MEDLINE
MEDLINE
PubMed
PubMed Central (Full Participant titles)
DatabaseTitle MEDLINE
Medline Complete
MEDLINE with Full Text
PubMed
MEDLINE (Ovid)
DatabaseTitleList

MEDLINE
Database_xml – sequence: 1
  dbid: NPM
  name: PubMed
  url: https://proxy.k.utb.cz/login?url=http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed
  sourceTypes: Index Database
– sequence: 2
  dbid: EIF
  name: MEDLINE
  url: https://proxy.k.utb.cz/login?url=https://www.webofscience.com/wos/medline/basic-search
  sourceTypes: Index Database
DeliveryMethod fulltext_linktorsrc
Discipline Chemistry
Biology
EISSN 1098-5549
EndPage 4831
ExternalDocumentID 8756641
12263552
mcb_16_9_4824
Genre Research Article
Research Support, U.S. Gov't, P.H.S
Journal Article
GrantInformation_xml – fundername: NIGMS NIH HHS
  grantid: GM40506
GroupedDBID ---
-DZ
-~X
.55
.GJ
08R
0R~
123
18M
29M
2WC
39C
3O-
4.4
53G
5RE
5VS
9M8
AAPBV
AAUGY
ABPTK
ACGFO
ACKIV
ACNCT
ADBBV
ADIYS
AENEX
AFFNX
AGVNZ
ALMA_UNASSIGNED_HOLDINGS
AOIJS
BAWUL
BTFSW
C1A
CS3
DIK
DU5
E3Z
EBS
EJD
F20
F5P
GX1
H13
HH5
HYE
HZ~
IH2
KQ8
L7B
MVM
N9A
O9-
OK1
P2P
RHF
RHI
RNS
RPM
RSF
TFL
TFW
TR2
UCJ
UDS
VQA
W8F
WH7
WOQ
X7M
Y6R
YYP
ZA5
ZCA
ZGI
ZXP
ABJNI
ABRLO
ABTAH
AEOZL
AGHSJ
CGR
CUY
CVF
ECM
EIF
EMOBN
M4Z
NPM
TDBHL
WHG
ZY4
5PM
ID FETCH-LOGICAL-h372t-24ec381b2fcff47fc350cdb59112ec941ddaf6ca963aac78b3033622e0a2267a3
IEDL.DBID RPM
ISSN 0270-7306
IngestDate Tue Sep 17 21:34:51 EDT 2024
Sat Sep 28 08:40:05 EDT 2024
Tue Jun 13 19:24:40 EDT 2023
Wed May 18 15:28:12 EDT 2016
IsDoiOpenAccess false
IsOpenAccess true
IsPeerReviewed true
IsScholarly true
Issue 9
Language English
LinkModel DirectLink
MergedId FETCHMERGED-LOGICAL-h372t-24ec381b2fcff47fc350cdb59112ec941ddaf6ca963aac78b3033622e0a2267a3
OpenAccessLink https://mcb.asm.org/content/mcb/16/9/4824.full.pdf
PMID 8756641
PageCount 8
ParticipantIDs pubmed_primary_8756641
informaworld_taylorfrancis_310_1128_MCB_16_9_4824
pubmedcentral_primary_oai_pubmedcentral_nih_gov_231484
highwire_asm_mcb_16_9_4824
PublicationCentury 1900
PublicationDate 19960901
9/1/1996
1996-Sep
PublicationDateYYYYMMDD 1996-09-01
PublicationDate_xml – month: 09
  year: 1996
  text: 19960901
  day: 01
PublicationDecade 1990
PublicationPlace United States
PublicationPlace_xml – name: United States
PublicationTitle Molecular and Cellular Biology
PublicationTitleAlternate Mol Cell Biol
PublicationYear 1996
Publisher American Society for Microbiology
Taylor & Francis
Publisher_xml – name: American Society for Microbiology
– name: Taylor & Francis
SSID ssj0006903
Score 1.8796599
Snippet Article Usage Stats Services MCB Citing Articles Google Scholar PubMed Related Content Social Bookmarking CiteULike Delicious Digg Facebook Google+ Mendeley...
The cmd1-6 allele contains three mutations that block Ca 2+ binding to calmodulin from Saccharomyces cerevisiae. We find that strains containing cmd1-6 lose...
The cmd1-6 allele contains three mutations that block Ca2+ binding to calmodulin from Saccharomyces cerevisiae. We find that strains containing cmd1-6 lose...
SourceID pubmedcentral
pubmed
informaworld
highwire
SourceType Open Access Repository
Index Database
Publisher
StartPage 4824
SubjectTerms Base Sequence
Calcineurin
Calcium - physiology
Calcium-Calmodulin-Dependent Protein Kinases - genetics
Calcium-Calmodulin-Dependent Protein Kinases - physiology
Calmodulin - genetics
Calmodulin - physiology
Calmodulin-Binding Proteins - physiology
Enzyme Activation
Fungal Proteins - physiology
Mating Factor
Molecular Sequence Data
Peptides - physiology
Phosphoprotein Phosphatases - physiology
Saccharomyces cerevisiae - drug effects
Saccharomyces cerevisiae - physiology
Sequence Deletion
Title Ca2+-calmodulin promotes survival of pheromone-induced growth arrest by activation of calcineurin and Ca2+-calmodulin-dependent protein kinase
URI http://mcb.asm.org/content/16/9/4824.abstract
https://www.tandfonline.com/doi/abs/10.1128/MCB.16.9.4824
https://www.ncbi.nlm.nih.gov/pubmed/8756641
https://pubmed.ncbi.nlm.nih.gov/PMC231484
Volume 16
hasFullText 1
inHoldings 1
isFullTextHit
isPrint
link http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV1Lb9QwEB6xlSp6QVCo2EIrH7ghZxPHcZwjRFQVVSuQqNSb5fjBrmjSFZse-if4zYydpNrtsdc4dizPxPPw528APgXKsgpNN80MwwDFC0u1YwW1mWdaaqOljSjfK3F-zb_fFDfjpbDNCKvsTLNKuts26VbLiK1ct2Yx4cQWPy5r9Em45IsZzMo8nyL0cffFaC-eKrMypai9YuLVZHJxWX9NMpFUCZeMH8A-uupChFLwj9TAT_hKt6zSLmJyywSdvYZXo-9IvgxzfAMvXHcI-0M1yYdDeFlPxdvewr9as88U17-9swFrTtYRduc2ZHOPmwOqF7nzJFAK4OPOUYzMUcaW_MaovF8SHUt2kOaBhHsPQ9Y2dMABw0F8yNAT3Vny5Ct0Kqnbk8j_gG_9WXVoJ9_B9dm3X_U5HUsv0GVesp4y7gza8oZ54z0vvcmL1NimwK2ROVPxzFrthdH4-2ptStmgJURTyFyq0Z8rdX4Eex1O_z0QWTmbF6UuMud4ikO4kHfKM9boxtmUz-F4Wn6lN61qTaMyoSoVpDSHbFsgqo9pDD_UHFF5jGCYVCjZ7T5Hg7DUeiDsUKOg5yB2pPjYHii2d1tQ8yLV9qBpx8_t-AEOBpR3wKR9hL3-7707QSemb05hdvFTnkbd_Q_FMvar
link.rule.ids 230,315,733,786,790,891,27955,27956,53825,53827
linkProvider National Library of Medicine
linkToHtml http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV1Lb9QwEB5BUdVeKBQqtlDwgRtyNnGc1xGiVgt0Kw5dqTfLr7CrknTFZg_lR_CbGTtJtbu3co1jx9GMPQ9__gbgo6MsK9B000gzDFCq1FBpWUJNVDGZSy1z41G-V-lkxr_dJDf9pbBVD6tstFoEza86aBZzj61c1no84MTGP6Yl-iQ85-On8AyXK0uGGL3ffzHe8-fKLAsp6m86MGuyfDwtvwRRGhQBzxk_hH101tPUFYN_IAfeYSzdsEvbmMkNI3RxBLNh-h325DZYtyrQf3aYHR_7fy_gee-Vks9d60t4Yptj2O_qVN4fw0E5lIV7BX9LyT5RlGx9ZxyKnSw9oM-uyGqN2w4qLrmriCMrwMeNpRjzo_YY8hPj_XZOpC8GQtQ9cTcqunyw64ADuiN-l_snsjFk5yt0KNbbEs8sgW_dLhq0wK9hdnF-XU5oX9SBzuOMtZRxq9FLUKzSVcWzSsdJqI1KcNNlVhc8MkZWqZa4MUips1yhjUUjy2wo0VPMZHwCew1O_w2QvLAmTjKZRNbyEIewLqMVR0xJZU3IR3A6iFXIVS1qrUSUikI46Y8g2hS0aH2CpOqqmYjYx0YsF6gxm31OOiUQy44KRPQKNIJ0Szse2h1593YLytyTeHcyPv3fjh_gYHI9vRSXX6--v4XDDkvukG_vYK_9vbZn6Cq16r1fGf8Aly4XgA
linkToPdf http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV3JbtswEB2kKdLm0iVtEKcbD70VlCWK2o6tWiNdEuTQAOmJ4FobqRQjlg_pR_SbO6SswPYxV1GkRMxwFvLxDcB7T1lWoeumiWaYoLjcUGlZRk3imCyllqUJKN-z_OSCf7vMLnegHO7CBNC-VrOo_dNE7WwasJXzRo8HnNj4_LTGmISXfDw3bvwAHuKSZcWQp69sMOZ84WyZFTFFHc4Hdk1Wjk_rT1GSR1XES8b3YQ8D9jz3BeHvCIK3WEvXfNMmbnLNEU2ewq9hCj3-5CpadirSf7fYHe8zx2fwZBWdko_9G89hx7YHsNfXq7w9gMf1UB7uBfyrJftAUcLNtfFodjIPwD67IIslmh9UYHLtiCctwMetpZj7oxYZ8hvz_m5KZCgKQtQt8Tcr-n1h3wEH9Ef9_gyAyNaQra_QoWhvRwLDBL51NWvRE7-Ei8mXn_UJXRV3oNO0YB1l3GqMFhRz2jleOJ1msTYqQ-PLrK54Yox0uZZoIKTURanQ16KzZTaWGDEWMj2E3RZ__whIWVmTZoXMEmt5jENYv7OVJkxJZU3MR3A8iFbIRSMarUSSi0p4DRhBsi5s0YWNEtdXNRFpyJFYKVBr1vsc9oog5j0liFgp0QjyDQ25a_ck3pstKPdA5t3L-fi-Hd_Bo_PPE_Hj69n3V7DfQ8o9AO417HY3S_sGI6ZOvQ2L4z8zrxoA
openUrl ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Ca2%2B-calmodulin+promotes+survival+of+pheromone-induced+growth+arrest+by+activation+of+calcineurin+and+Ca2%2B-calmodulin-dependent+protein+kinase&rft.jtitle=Molecular+and+Cellular+Biology&rft.au=M+J+Moser&rft.au=J+R+Geiser&rft.au=T+N+Davis&rft.date=1996-09-01&rft.pub=American+Society+for+Microbiology&rft.issn=0270-7306&rft.eissn=1098-5549&rft.volume=16&rft.issue=9&rft.spage=4824&rft_id=info:doi/10.1128%2FMCB.16.9.4824&rft_id=info%3Apmid%2F8756641&rft.externalDBID=n%2Fa&rft.externalDocID=mcb_16_9_4824
thumbnail_l http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=0270-7306&client=summon
thumbnail_m http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=0270-7306&client=summon
thumbnail_s http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=0270-7306&client=summon