Effect of Ochratoxin a on human haematopoietic progenitors proliferation and differentiation: an in vitro study

• Published in: Human & experimental toxicology Vol. 22; no. 7; pp. 393 - 400
• Main Authors: Froquet, R, Le Dréan, G, Parent-Massin, D
• Format: Journal Article
• Language: English
• Published: Thousand Oaks, CA SAGE Publications 01.07.2003; Arnold; Sage Publications Ltd
• Subjects: Biological and medical sciences; Cell Differentiation - drug effects; Cell Division - drug effects; Cells, Cultured; Erythroid Precursor Cells - cytology; Erythroid Precursor Cells - drug effects; Erythroid Precursor Cells - metabolism; Food and Nutrition; Hematopoiesis - drug effects; Humans; Life Sciences; Medical sciences; Megakaryocytes - cytology; Megakaryocytes - drug effects; Megakaryocytes - metabolism; Mycotoxins - toxicity; Ochratoxins - toxicity; Plant poisons toxicology; Toxicology; haematopoietic progenitors; myelotoxicity; ochratoxin A; human; Human; Cell proliferation; Cell culture; Ochratoxin; Toxicity; Mycotoxin; Cell differentiation; In vitro; Bone marrow disease; Toxin; Hematopoiesis; Bone marrow; Progenitor cell; Mycotoxins/toxicity; Megakaryocytes/metabolism; Megakaryocytes/drug effects; Megakaryocytes/cytology; Hematopoiesis/drug effects; Ochratoxins/toxicity
• ISSN: 0960-3271; 1477-0903
• DOI: 10.1191/0960327103ht375oa

Ochratoxin A (OTA) is a mycotoxin food and feedcontaminant known to induce nephro and hepatotoxicity in human and animal, and related to human Balkan Endemic Nephropathy. However, haematological troubles are also observed in case of acute OTA intoxication. These disorders observed in animals emphasise the necessity to determine if OTA exposure induce damage to haematopoietic system in human. The effect on haematopoiesis has been evaluated usingin vitro clonogenic assays of the three lineages i.e., platelet, red and white blood cell progenitors. Human erythroblastic and granulomonocytic progenitor proliferation is decreased in the presence of 102 mM OTA. Platelet progenitors were destroyed at 102 mM OTA. For the lowest concentrations haematopoietic progenitor proliferation is not affected by OTA. Comparison with other mycotoxins known to be myelotoxic shows that OTA is less myelotoxic than trichothecenes.