Phylogenetic relationships in the genus Leonardoxa (Leguminosae: Caesalpinioideae) inferred from chloroplast trnL intron and trnL-trnF intergenic spacer sequences

The African genus Leonardoxa (Leguminosae: Caesalpinioideae) comprises two Congolean species and a group of four mostly allopatric subspecies principally located in Cameroon and clustered together in the L. africana complex. Leonardoxa provides a good opportunity to investigate the evolutionary hist...

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Published inAmerican journal of botany Vol. 88; no. 1; pp. 143 - 149
Main Authors Brouat, Carine, Gielly, Ludovic, McKey, Doyle
Format Journal Article
LanguageEnglish
Published United States Botanical Soc America 01.01.2001
Botanical Society of America
Botanical Society of America, Inc
Subjects
Ants
Biodiversity
Biodiversity and Ecology
Biological taxonomies
Botany
Caesalpinioideae
Chloroplast DNA
cpDNA
Deoxyribonucleic acid
DNA
Ecology, environment
Environmental Sciences
Evolution
Flowers & plants
Genetics
Insect genetics
introgression
Leguminosae
Leonardoxa
Life Sciences
myrmecophytes
Phylogenetics
Phylogeny
Plant morphology
Plants
Systematics
Taxa
universal primers
Leonardoxa
dna phylogeography
asteraceae
oaks
rbcl
cpDNA
introgression
Caesalpinioideae
amplification
Leguminosae
plants
phylogeny
nuclear
myrmecophytes
Noncoding regions
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Summary:The African genus Leonardoxa (Leguminosae: Caesalpinioideae) comprises two Congolean species and a group of four mostly allopatric subspecies principally located in Cameroon and clustered together in the L. africana complex. Leonardoxa provides a good opportunity to investigate the evolutionary history of ant-plant mutualisms, as it exhibits various grades of ant-plant interactions from diffuse to obligate and symbiotic associations. We present in this paper the first molecular phylogenetic study of this genus. We sequenced both the chloroplast DNA trnL intron (677 aligned base pairs [bp]) and trnL-trnF intergene spacer (598 aligned bp). Inferred phylogenetic relationships suggested first that the genus is paraphyletic. The L. africana complex is clearly separated from the two Congolean species, and the integrity of the genus is thus in question. In the L. africana complex, our data showed a lack of congruence between clades suggested by morphological and chloroplast characters. This, and the low level of molecular divergence found between subspecies, suggests gene flow and introgressive events in the L. africana complex.
Bibliography:Loesenera kalantha
Marc Colyn and Annie Gautier‐Hion (Station Biologique de Paimpont, CNRS) for collecting the
L. bequaertii
The authors thank the Ministry of Research and Higher Education of the Republic of Cameroon for permission to carry out research in Cameroon, Alain Ngomi for providing help in collection of samples, Edmond Dounias for the loan of a field vehicle, Anne Bruneau for providing leaf samples from
(collected by Frans Breteler and J. J. deWilde) and a DNA sample from
Author for reprint requests (e‐mail
used in preparing a DNA sample, Marie‐Hélène Pemonge and Chantal Debain for technical advice and assistance in the laboratory, Frans Breteler for identifying our specimens of outgroup taxa, Anne Bruneau, Rémy Petit, and Pierre Taberlet for helpful discussions, and Bernard Angers, Jean‐François Cosson, Emmanuel Douzery, Philippe Jarne, Emmanuelle Jousselin, Yan Linhart, John Thompson, and two reviewers for their helpful comments on various drafts of the manuscript. This paper is publication number 2000–116 of the Institut des Sciences de l'Evolution, Montpellier. The research was funded by the programme “Environnement, Vie et Sociétés” of the CNRS.
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L. romii
brouat@cefe.cnrs‐mop.fr
ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0002-9122
1537-2197
DOI:10.2307/2657134