Vpu and Tsg101 Regulate Intracellular Targeting of the Human Immunodeficiency Virus Type 1 Core Protein Precursor Pr55gag
Article Usage Stats Services JVI Citing Articles Google Scholar PubMed Related Content Social Bookmarking CiteULike Delicious Digg Facebook Google+ Mendeley Reddit StumbleUpon Twitter current issue Spotlights in the Current Issue JVI About JVI Subscribers Authors Reviewers Advertisers Inquiries from...
Saved in:
| Published in | Journal of Virology Vol. 80; no. 8; pp. 3765 - 3772 |
|---|---|
| Main Authors | , , , , , |
| Format | Journal Article |
| Language | English |
| Published |
Washington, DC
American Society for Microbiology
01.04.2006
|
| Subjects | |
| Online Access | Get full text |
Cover
Loading…
| Abstract | Article Usage Stats
Services
JVI
Citing Articles
Google Scholar
PubMed
Related Content
Social Bookmarking
CiteULike
Delicious
Digg
Facebook
Google+
Mendeley
Reddit
StumbleUpon
Twitter
current issue
Spotlights in the Current Issue
JVI
About
JVI
Subscribers
Authors
Reviewers
Advertisers
Inquiries from the Press
Permissions & Commercial Reprints
ASM Journals Public Access Policy
JVI
RSS Feeds
1752 N Street N.W. • Washington DC 20036
202.737.3600 • 202.942.9355 fax • journals@asmusa.org
Print ISSN:
0022-538X
Online ISSN:
1098-5514
Copyright © 2014
by the
American Society for Microbiology.
For an alternate route to
JVI
.asm.org, visit:
JVI
|
|---|---|
| AbstractList | Assembly of human immunodeficiency virus type 1 (HIV-1) is directed by the viral core protein Pr55gag. Depending on the cell type, Pr55gag accumulates either at the plasma membrane or on late endosomes/multivesicular bodies. Intracellular localization of Pr55gag determines the site of virus assembly, but molecular mechanisms that define cell surface or endosomal targeting of Pr55gag are poorly characterized. We have analyzed targeting of newly synthesized Pr55gag in HeLa H1 cells by pulse-chase studies and subcellular fractionations. Our results indicated that Pr55gag was inserted into the plasma membrane and, when coexpressed with the viral accessory protein Vpu, Pr55gag remained at the plasma membrane and virions assembled at this site. In contrast, Pr55gag expressed in the absence of Vpu was initially inserted into the plasma membrane, but subsequently endocytosed, and virus assembly was partially shifted to internal membranes. This endocytosis of Pr55gag required the host protein Tsg101. These results identified a previously unknown role for Vpu and Tsg101 as regulators for the endocytic uptake of Pr55gag and suggested that the site of HIV-1 assembly is determined by factors that regulate the endocytosis of Pr55gag. Assembly of human immunodeficiency virus type 1 (HIV-1) is directed by the viral core protein Pr55 gag . Depending on the cell type, Pr55 gag accumulates either at the plasma membrane or on late endosomes/multivesicular bodies. Intracellular localization of Pr55 gag determines the site of virus assembly, but molecular mechanisms that define cell surface or endosomal targeting of Pr55 gag are poorly characterized. We have analyzed targeting of newly synthesized Pr55 gag in HeLa H1 cells by pulse-chase studies and subcellular fractionations. Our results indicated that Pr55 gag was inserted into the plasma membrane and, when coexpressed with the viral accessory protein Vpu, Pr55 gag remained at the plasma membrane and virions assembled at this site. In contrast, Pr55 gag expressed in the absence of Vpu was initially inserted into the plasma membrane, but subsequently endocytosed, and virus assembly was partially shifted to internal membranes. This endocytosis of Pr55 gag required the host protein Tsg101. These results identified a previously unknown role for Vpu and Tsg101 as regulators for the endocytic uptake of Pr55 gag and suggested that the site of HIV-1 assembly is determined by factors that regulate the endocytosis of Pr55 gag . Assembly of human immunodeficiency virus type 1 (HIV-1) is directed by the viral core protein Pr55gag. Depending on the cell type, Pr55 gag accumulates either at the plasma membrane or on late endosomes/multivesicular bodies. Intracellular localization of Pr55 gag determines the site of virus assembly, but molecular mechanisms that define cell surface or endosomal targeting of Pr55gag are poorly characterized. We have analyzed targeting of newly synthesized Pr55 gag in HeLa H1 cells by pulse-chase studies and subcellular fractionations. Our results indicated that Pr55gag was inserted into the plasma membrane and, when coexpressed with the viral accessory protein Vpu, Pr55gag remained at the plasma membrane and virions assembled at this site. In contrast, Pr55gag expressed in the absence of Vpu was initially inserted into the plasma membrane, but subsequently endocytosed, and virus assembly was partially shifted to internal membranes. This endocytosis of Pr55gag required the host protein Tsg101. These results identified a previously unknown role for Vpu and Tsg101 as regulators for the endocytic uptake of Pr55gag and suggested that the site of HIV-1 assembly is determined by factors that regulate the endocytosis of Pr55gag. Copyright © 2006, American Society for Microbiology. All Rights Reserved. Article Usage Stats Services JVI Citing Articles Google Scholar PubMed Related Content Social Bookmarking CiteULike Delicious Digg Facebook Google+ Mendeley Reddit StumbleUpon Twitter current issue Spotlights in the Current Issue JVI About JVI Subscribers Authors Reviewers Advertisers Inquiries from the Press Permissions & Commercial Reprints ASM Journals Public Access Policy JVI RSS Feeds 1752 N Street N.W. • Washington DC 20036 202.737.3600 • 202.942.9355 fax • journals@asmusa.org Print ISSN: 0022-538X Online ISSN: 1098-5514 Copyright © 2014 by the American Society for Microbiology. For an alternate route to JVI .asm.org, visit: JVI |
| Author | Jorma Hinkula Ian Prior Maarit Suomalainen Kirsi Harila Antti Salminen Mathilda Sjöberg |
| AuthorAffiliation | Department of Virology, Haartman Institute, FIN-00014 University of Helsinki, Finland, 1 The Physiological Laboratory, University of Liverpool, Liverpool L69 3BX, United Kingdom, 3 Department of Biosciences at Novum, Karolinska Institutet, S-141 57 Huddinge, Sweden, 2 Department of Virology, Swedish Institute for Infectious Disease Control and MTC, Karolinska Institutet, 171 82 Stockholm and Department of Molecular Virology, Linkoping University, 581 83 Linkoping, Sweden 4 |
| AuthorAffiliation_xml | – name: Department of Virology, Haartman Institute, FIN-00014 University of Helsinki, Finland, 1 The Physiological Laboratory, University of Liverpool, Liverpool L69 3BX, United Kingdom, 3 Department of Biosciences at Novum, Karolinska Institutet, S-141 57 Huddinge, Sweden, 2 Department of Virology, Swedish Institute for Infectious Disease Control and MTC, Karolinska Institutet, 171 82 Stockholm and Department of Molecular Virology, Linkoping University, 581 83 Linkoping, Sweden 4 |
| Author_xml | – sequence: 1 givenname: Kirsi surname: HARILA fullname: HARILA, Kirsi organization: Department of Virology, Haartman Institute, University of Helsinki, 00014, Finland – sequence: 2 givenname: Ian surname: PRIOR fullname: PRIOR, Ian organization: Physiological Laboratory, University of Liverpool, Liverpool L69 3BX, United Kingdom – sequence: 3 givenname: Mathilda surname: SJÖBERG fullname: SJÖBERG, Mathilda organization: Department of Biosciences at Novum, Karolinska Institutet, 141 57 Huddinge, Sweden – sequence: 4 givenname: Antti surname: SALMINEN fullname: SALMINEN, Antti organization: Department of Virology, Haartman Institute, University of Helsinki, 00014, Finland – sequence: 5 givenname: Jorma surname: HINKULA fullname: HINKULA, Jorma organization: Department of Virology, Swedish Institute for Infectious Disease Control and MTC, Karolinska Institutet, 171 82 Stockholm, Sweden – sequence: 6 givenname: Maarit surname: SUOMALAINEN fullname: SUOMALAINEN, Maarit organization: Department of Virology, Haartman Institute, University of Helsinki, 00014, Finland |
| BackLink | http://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=17650397$$DView record in Pascal Francis https://www.ncbi.nlm.nih.gov/pubmed/16571793$$D View this record in MEDLINE/PubMed https://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-34583$$DView record from Swedish Publication Index http://kipublications.ki.se/Default.aspx?queryparsed=id:1932084$$DView record from Swedish Publication Index |
| BookMark | eNp1kt9v0zAQxy00xLrBvwDmAZ5IseM4dl4mTWWwokkgVCreLMe5ph6JXexkU__7OWr5sQcerLuzP3df23dn6MR5Bwi9omROaS7ff14v55LM5ZyJkmdMiHyeE1I-QTNKKplxTosTNCMkzzPO5I9TdBbjLSG0KMriGTqlJRdUVGyG9uvdiLVr8Cq2lFD8Ddqx0wPgpRuCNtB1KQx4pUMLg3Ut9hs8bAFfj712eNn3o_MNbKyx4Mwer20YI17td4ApXvgA-GvwA1iXLJgxRB-Sx3mr2-fo6UZ3EV4c7Tn6_vFqtbjObr58Wi4ub7Ito2WR1XVNNK0pVLygZZ1eIwTRXNYyT0FOKwkFrQxsDK81lVAZ2tTMSJBNAgVj50gc6sZ72I212gXb67BXXtvk-0Yd93_aaakIilYsJ7JIme_-m_nBri-VD63q7KhYweUkdHHAE9tDY2D6we6x3qMTZ7eq9XcqdYUUkqYCb48Fgv81QhxUb-PUAu3Aj1GVQlJO-HSxl_8q_ZH43dYEvDkCOhrdbYJ2xsa_XBoawiqRuNcHbmvb7b0NoHTs1e2dVZIoqabhYg81JMKh |
| ContentType | Journal Article |
| Copyright | 2006 INIST-CNRS Copyright © 2006, American Society for Microbiology 2006 |
| Copyright_xml | – notice: 2006 INIST-CNRS – notice: Copyright © 2006, American Society for Microbiology 2006 |
| DBID | IQODW CGR CUY CVF ECM EIF NPM 7X8 5PM ADTPV AOWAS DG8 D8T ZZAVC |
| DOI | 10.1128/JVI.80.8.3765-3772.2006 |
| DatabaseName | Pascal-Francis Medline MEDLINE MEDLINE (Ovid) MEDLINE MEDLINE PubMed MEDLINE - Academic PubMed Central (Full Participant titles) SwePub SwePub Articles SWEPUB Linköpings universitet SWEPUB Freely available online SwePub Articles full text |
| DatabaseTitle | MEDLINE Medline Complete MEDLINE with Full Text PubMed MEDLINE (Ovid) MEDLINE - Academic |
| DatabaseTitleList | MEDLINE - Academic MEDLINE |
| Database_xml | – sequence: 1 dbid: NPM name: PubMed url: https://proxy.k.utb.cz/login?url=http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed sourceTypes: Index Database – sequence: 2 dbid: EIF name: MEDLINE url: https://proxy.k.utb.cz/login?url=https://www.webofscience.com/wos/medline/basic-search sourceTypes: Index Database |
| DeliveryMethod | fulltext_linktorsrc |
| Discipline | Biology Medicine |
| EISSN | 1098-5514 |
| EndPage | 3772 |
| ExternalDocumentID | oai_prod_swepub_kib_ki_se_1932084 oai_DiVA_org_liu_34583 16571793 17650397 jvi_80_8_3765 |
| Genre | Research Support, Non-U.S. Gov't Journal Article |
| GroupedDBID | --- -~X .55 .GJ 08R 0R~ 18M 29L 2WC 39C 3O- 4.4 41~ 53G 5GY 5RE 5VS 6TJ 85S AAUGY AAYJJ ABPPZ ABPTK ACGFO ACNCT ADBBV AENEX AFDAS AFFNX AI. ALMA_UNASSIGNED_HOLDINGS AOIJS BAWUL BTFSW C1A CS3 D0S DIK E3Z EBS EJD F20 F5P FRP GX1 H13 HYE HZ~ IH2 IQODW KQ8 MVM N9A O9- OHT OK1 P2P RHF RHI RNS RPM RSF TR2 UCJ UPT VH1 W2D W8F WH7 WOQ X7M XFK Y6R YQT ZA5 ZGI ZXP ~02 ~KM AGVNZ CGR CUY CVF ECM EIF NPM 7X8 5PM ADTPV AOWAS DG8 D8T ZZAVC |
| ID | FETCH-LOGICAL-h3164-bbb0a1b1e95416b538770a58b82b532198e419cefc5ba18e9c1db3c8e8d770733 |
| IEDL.DBID | RPM |
| ISSN | 0022-538X 1098-5514 |
| IngestDate | Sun Oct 27 03:41:47 EDT 2024 Tue Oct 01 22:47:51 EDT 2024 Tue Sep 17 21:21:31 EDT 2024 Sun Sep 22 05:14:45 EDT 2024 Sat Sep 28 07:47:05 EDT 2024 Sun Oct 22 16:04:33 EDT 2023 Wed May 18 15:32:09 EDT 2016 |
| IsDoiOpenAccess | true |
| IsOpenAccess | true |
| IsPeerReviewed | true |
| IsScholarly | true |
| Issue | 8 |
| Keywords | Immunopathology Targeting Microbiology HIV-1 virus Retroviridae AIDS Immune deficiency Lentivirus Protein Virology Infection Virus Regulation(control) Viral disease Human immunodeficiency virus Intracellular |
| Language | English |
| License | CC BY 4.0 |
| LinkModel | DirectLink |
| MergedId | FETCHMERGED-LOGICAL-h3164-bbb0a1b1e95416b538770a58b82b532198e419cefc5ba18e9c1db3c8e8d770733 |
| Notes | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 Corresponding author. Mailing address: Department of Virology, Haartman Institute, P.O. Box 21, FIN-00014 University of Helsinki, Finland. Phone: 358-9-19126688. Fax: 358-9-19126491. E-mail: maarit.suomalainen@helsinki.fi. |
| OpenAccessLink | http://kipublications.ki.se/Default.aspx?queryparsed=id:1932084 |
| PMID | 16571793 |
| PQID | 67815054 |
| PQPubID | 23479 |
| PageCount | 8 |
| ParticipantIDs | swepub_primary_oai_DiVA_org_liu_34583 highwire_asm_jvi_80_8_3765 proquest_miscellaneous_67815054 pascalfrancis_primary_17650397 pubmedcentral_primary_oai_pubmedcentral_nih_gov_1440481 swepub_primary_oai_prod_swepub_kib_ki_se_1932084 pubmed_primary_16571793 |
| PublicationCentury | 2000 |
| PublicationDate | 20060401 |
| PublicationDateYYYYMMDD | 2006-04-01 |
| PublicationDate_xml | – month: 4 year: 2006 text: 20060401 day: 1 |
| PublicationDecade | 2000 |
| PublicationPlace | Washington, DC |
| PublicationPlace_xml | – name: Washington, DC – name: United States |
| PublicationTitle | Journal of Virology |
| PublicationTitleAlternate | J Virol |
| PublicationYear | 2006 |
| Publisher | American Society for Microbiology |
| Publisher_xml | – name: American Society for Microbiology |
| References | 14617353 - Traffic. 2003 Dec;4(12):902-10 1718346 - AIDS Res Hum Retroviruses. 1991 Aug;7(8):707-12 8139035 - J Virol. 1994 Apr;68(4):2556-69 12230470 - Traffic. 2002 Oct;3(10):718-29 15099524 - Mol Cell. 2004 Apr 23;14(2):259-67 3260631 - J Virol. 1988 Aug;62(8):2578-86 2479031 - Proc Natl Acad Sci U S A. 1989 Nov;86(22):8964-7 15767407 - J Virol. 2005 Apr;79(7):4055-65 1694957 - Mol Immunol. 1990 May;27(5):395-403 12947040 - Proc Natl Acad Sci U S A. 2003 Sep 16;100(19):10592-7 8151785 - J Virol. 1994 May;68(5):3232-42 15920469 - EMBO J. 2005 Jul 6;24(13):2481-9 11145902 - Virology. 2001 Jan 5;279(1):201-9 6309765 - J Biol Chem. 1983 Aug 25;258(16):10062-72 3194424 - Proc Natl Acad Sci U S A. 1988 Dec;85(23):9283-6 14617360 - Traffic. 2003 Nov;4(11):785-801 3643678 - Virology. 1987 Jan;156(1):171-6 2676191 - Cell. 1989 Oct 6;59(1):103-12 14722309 - J Virol. 2004 Feb;78(3):1552-63 15766529 - Cell. 2005 Mar 11;120(5):663-74 12885763 - J Cell Biol. 2003 Aug 4;162(3):443-55 12915533 - J Virol. 2003 Sep;77(17):9173-82 11726971 - Nat Med. 2001 Dec;7(12):1313-9 2788277 - Proc Natl Acad Sci U S A. 1989 Aug;86(15):5781-5 14657387 - Proc Natl Acad Sci U S A. 2003 Dec 9;100(25):15154-9 2405382 - Proc Natl Acad Sci U S A. 1990 Jan;87(2):523-7 10954568 - J Virol. 2000 Sep;74(18):8670-9 11595185 - Cell. 2001 Oct 5;107(1):55-65 14505570 - Cell. 2003 Sep 19;114(6):701-13 11427703 - Proc Natl Acad Sci U S A. 2001 Jul 3;98(14):7724-9 10846064 - J Virol. 2000 Jul;74(13):5845-55 15473846 - Annu Rev Cell Dev Biol. 2004;20:395-425 12941395 - Microbes Infect. 2003 Sep;5(11):1029-39 2404139 - J Virol. 1990 Feb;64(2):621-9 9306402 - Nat Biotechnol. 1997 Sep;15(9):871-5 6095449 - Science. 1984 Dec 7;226(4679):1165-71 3016298 - J Virol. 1986 Aug;59(2):284-91 11878889 - Virology. 2001 Dec 20;291(2):198-207 10753709 - Virology. 2000 Apr 10;269(2):305-12 12663787 - J Virol. 2003 Apr;77(8):4805-17 7979256 - Annu Rev Biochem. 1994;63:869-914 10708443 - J Virol. 2000 Apr;74(7):3264-72 8991095 - J Cell Biol. 1996 Dec;135(6 Pt 2):1841-52 14681580 - RNA. 2004 Jan;10(1):12-8 14769924 - Proc Natl Acad Sci U S A. 2004 Feb 17;101(7):1892-7 7859737 - EMBO J. 1995 Feb 1;14(3):484-91 8346259 - Proc Natl Acad Sci U S A. 1993 Aug 1;90(15):7381-5 |
| References_xml | |
| SSID | ssj0014464 |
| Score | 1.8486605 |
| Snippet | Article Usage Stats
Services
JVI
Citing Articles
Google Scholar
PubMed
Related Content
Social Bookmarking
CiteULike
Delicious
Digg
Facebook
Google+
Mendeley... Assembly of human immunodeficiency virus type 1 (HIV-1) is directed by the viral core protein Pr55gag. Depending on the cell type, Pr55gag accumulates either... Assembly of human immunodeficiency virus type 1 (HIV-1) is directed by the viral core protein Pr55 gag . Depending on the cell type, Pr55 gag accumulates... Assembly of human immunodeficiency virus type 1 (HIV-1) is directed by the viral core protein Pr55gag. Depending on the cell type, Pr55 gag accumulates either... |
| SourceID | swepub pubmedcentral proquest pubmed pascalfrancis highwire |
| SourceType | Open Access Repository Aggregation Database Index Database Publisher |
| StartPage | 3765 |
| SubjectTerms | Biological and medical sciences Cell Membrane - metabolism DNA-Binding Proteins - physiology Endocytosis Endosomal Sorting Complexes Required for Transport Fundamental and applied biological sciences. Psychology Gene Products, gag - metabolism HeLa Cells HIV-1 - physiology Human Immunodeficiency Virus Proteins Human viral diseases Humans Infectious diseases Medical sciences MEDICIN Medicin och hälsovetenskap MEDICINE Microbiology Miscellaneous Protein Precursors - metabolism Structure and Assembly Transcription Factors - physiology Viral diseases Viral diseases of the lymphoid tissue and the blood. Aids Viral Regulatory and Accessory Proteins - physiology Virion - physiology Virology Virus Assembly |
| Title | Vpu and Tsg101 Regulate Intracellular Targeting of the Human Immunodeficiency Virus Type 1 Core Protein Precursor Pr55gag |
| URI | http://jvi.asm.org/content/80/8/3765.abstract https://www.ncbi.nlm.nih.gov/pubmed/16571793 https://search.proquest.com/docview/67815054 https://pubmed.ncbi.nlm.nih.gov/PMC1440481 https://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-34583 http://kipublications.ki.se/Default.aspx?queryparsed=id:1932084 |
| Volume | 80 |
| hasFullText | 1 |
| inHoldings | 1 |
| isFullTextHit | |
| isPrint | |
| link | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV3Pb5swFH5qK03aZdrv0W2ZD9uRBAIGc6yyVU2nTtWURrlZtjEpXQMRlEn97_ceP7ah9bRDlBAMAj8_-3v25-8BfPQyxMShid0sEMoNESO7STpXrtEIXilu9jTtRr74Fp1dhecbvjkAPuyFaUn7RufT4nY3LfLrllu535nZwBObXV4saEEyFP7sEA7jIBhC9H7pAOObcJAIR2_e9KQu7Idn5-vlVHhTMUWv4uhacatE2WYxinhMTfUvmWBiSaoaKyrrMlw8BEH_ZVKO9EbbMer0KTzpwSU76V7iGRzY4jk86tJN3r-A-_W-YapI2areoo-w710eesuWdFuawidOKlu15HAc0liZMcSHrJ3oZ0vaSVKmliQnaL8mW-dVUzMKZJnPFmVl2SWJPuQFftMsfl1W-Ivzrdq-hKvTL6vFmdunXnCvAwygXK21p3zt24QjYtNYj3HsKS60mOMB9nLChn5ibGa4Vr6wifFTHRhhRYoF0Siv4KgoC_sGmI7nWRqhq2casVecqrlOTBSlXmQDo3XkwPFQ5VLVO3nzM5fCk0KSkRyYjIwg950Gh_TxpIdYyoEPg1UkOgZVlSps2dQSR2EEuzx04HVnoz_X9sZ2IB5Z73cBktwen8GW2Epv9y3PgU-dnUeXfM7XJ7KstvI2b2RAC9IOeA-Uo6FR9v__yOkjaysJSXsiPP7vh3oLj7v5IaIVvYOju6qx7xEx3ekJxgrLr5PWT34BonUYOA |
| link.rule.ids | 230,315,733,786,790,891,27946,27947,53816,53818 |
| linkProvider | National Library of Medicine |
| linkToHtml | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV1Lb9NAEB6VIkQvvEtdoN0DHO3YsdfeHKtAlZSmqlAa5bbatdepaWNHcYxUfj0zfgCBXuAQ5bFrydnZ2f3G8-03AO_dFDFxEEd26gtlB4iR7UHSV3asEbxS3OxqOo08uQhHV8HZnM93gHdnYWrSfqwzJ79dOnl2XXMrV8u41_HEepeTISUkA-H1HsBDTomvLkhvkwcY4QSdSDi2z1taF67EvbPZ2BGuIxz0K47OFdValHUdo5BHNFl_EwomnqQqcajSpsbFfSD0by7lluJovUudPoVZ9_8acsqNU220E3__Q_rxnwfgGTxpcSs7aZqfw47JX8CjppLl3Uu4m60qpvKETcsFuh_70pS4N2xM90vZAaK7smnNO8fdkhUpQ-jJ6hwCG9MhlSIxpGZBR0HZLFtXJaMYmXlsWKwNuyQ9iSzHd0oQlMUaP3G-UItXcHX6aToc2W1VB_vax9jM1lq7ytOeGXAEgxoNFEWu4kKLPn7BBVSYwBvEJo25Vp4wg9hLtB8LIxLsGPn-PuzmRW4OgOmonyYhriKpRlgXJaqvB3EYJm5o_Fjr0ILDzpZSlUv59VsmhSuFJOtbcLRlXblq5D2kh40uwjQLjjtzS_Q5GiqVm6IqJW7wiKN5YMHrxvi_rm1nkQXR1rT42YHUvLdb0Ma1qndrUws-NBNo65KP2exEFuuFvM0q6VOu2wL3nn6068r295uMXrI0kkC6K4LD_76pY3g8mk7O5fn44vMb2GseQxF76S3sbtaVeYfAbKOPajf8AV1sOSs |
| linkToPdf | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV1Lb9NAEB5BEYhLeVMXaPcARzt2_Nocq5SoKbSKUBpFXFa763Vq2thRHCOVX8-MH9BATz1EeexacnZmdr7xzH4D8NFNERMHOrZTn0s7QIxsD5K-tLVC8Epxs6voNPLZeXRyEZzOw_mtVl910b5WmZNfL508u6xrK1dL3evqxHqTsyElJAPu9VZJ2nsIj0LyOl2g3iYQMMoJOqJwHJ-3pV24G_dOZ2OHuw530LZCNLC45qOsexlFYUwKe4ssmGolZYnLlTZ9Lu4Cov_XU26xjtaeavQMvnf_sSlQuXKqjXL0r3_oH--1CM9ht8Wv7KiZ8gIemPwlPG46Wt68gpvZqmIyT9i0XKAZsm9Nq3vDxnTPlCWgslc2revP0WuyImUIQVmdS2BjOqxSJIZYLehIKJtl66pkFCszjw2LtWET4pXIcnynREFZrPFTGC7k4jVcjD5Phyd2293BvvQxRrOVUq70lGcGIYJChUKKY1eGXPE-fsGNlJvAG2iT6lBJj5uB9hLla254ghNj338DO3mRmz1gKu6nSYS7SaoQ3sWJ7KuBjqLEjYyvlYos2O_kKWS5FD9-ZoK7ggvSAAsOtiQsVg3Nh_Bw0EW4ZsFhJ3KBtkdLJXNTVKVAR494OgwseNsowN9rW02yIN5SjT8TiNV7ewTlXLN7t3K14FOjRFuXHGezI1GsF-I6q4RPOW8L3DvmkfcV7e9XGb1EaQSBdZcH-_e-qUN4Mjkeia_j8y_v4GnzNIqKmN7DzmZdmQ-IzzbqoLbE3wRqO6s |
| openUrl | ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Vpu+and+Tsg101+Regulate+Intracellular+Targeting+of+the+Human+Immunodeficiency+Virus+Type+1+Core+Protein+Precursor+Pr55gag&rft.jtitle=Journal+of+Virology&rft.au=Kirsi+Harila&rft.au=Ian+Prior&rft.au=Mathilda+Sj%C3%B6berg&rft.au=Antti+Salminen&rft.date=2006-04-01&rft.pub=American+Society+for+Microbiology&rft.issn=0022-538X&rft.eissn=1098-5514&rft.volume=80&rft.issue=8&rft.spage=3765&rft_id=info:doi/10.1128%2FJVI.80.8.3765-3772.2006&rft_id=info%3Apmid%2F16571793&rft.externalDBID=n%2Fa&rft.externalDocID=jvi_80_8_3765 |
| thumbnail_l | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=0022-538X&client=summon |
| thumbnail_m | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=0022-538X&client=summon |
| thumbnail_s | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=0022-538X&client=summon |