Molecular genetic alterations in superficial and locally advanced human bladder cancer

We attempted to define the role of tumor suppressor genes in the pathogenesis of human bladder cancer through a combined molecular genetic and immunohistochemical approach. Thirty-four bladder tumors (1 Pis, 6 Pa, 5 P1, 3 P3a, 18 P3b, 1 P4; 8 low grade and 26 high grade tumors) have been analyzed. R...

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Published inCancer research (Chicago, Ill.) Vol. 51; no. 19; pp. 5405 - 5409
Main Authors PRESTI, J. C, REUTER, V. E, GALAN, T, FAIR, W. R, CORDON-CARDO, C
Format Journal Article
LanguageEnglish
Published Philadelphia, PA American Association for Cancer Research 01.10.1991
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Abstract We attempted to define the role of tumor suppressor genes in the pathogenesis of human bladder cancer through a combined molecular genetic and immunohistochemical approach. Thirty-four bladder tumors (1 Pis, 6 Pa, 5 P1, 3 P3a, 18 P3b, 1 P4; 8 low grade and 26 high grade tumors) have been analyzed. Restriction fragment length polymorphism analysis directed at 5 suspected or established tumor suppressor gene regions (3p21-25, 11p15, 13q14, 17p11-13, and 18q21) was combined with immunohistochemical using Rb-PMG3-245 monoclonal antibody directed at the retinoblastoma (Rb) gene product. Tumor grade correlated with deletions of 3p (P = 0.004) and 17p (P = 0.063). Tumor stage correlated with deletions of 3p (P = 0.010), 17p (P = 0.015) and altered Rb expression (P = 0.054). Vascular invasion correlated only with deletions of 17p (P = 0.038). No marker correlated with positive lymph nodes. Our results suggest that altered Rb expression occurs in all grades and stages of bladder cancer but is more commonly associated with invasive tumors. Genetic alterations of 3p, 11p, 17p, and 18q are rare events in low grade, superficial tumors, whereas they are more common in high grade and invasive bladder cancer. The role of these genetic alterations in the prognosis of bladder cancer will require additional follow-up and further studies.
AbstractList We attempted to define the role of tumor suppressor genes in the pathogenesis of human bladder cancer through a combined molecular genetic and immunohistochemical approach. Our results suggest that altered Rb expression occurs in all grades and stages of bladder cancer but is more commonly associated with invasive tumors. Genetic alterations of 3p, 11p, 17p, and 18q are rare events in low grade, superficial tumors, whereas they are more common in high grade and invasive bladder cancer.
We attempted to define the role of tumor suppressor genes in the pathogenesis of human bladder cancer through a combined molecular genetic and immunohistochemical approach. Thirty-four bladder tumors (1 Pis, 6 Pa, 5 P1, 3 P3a, 18 P3b, 1 P4; 8 low grade and 26 high grade tumors) have been analyzed. Restriction fragment length polymorphism analysis directed at 5 suspected or established tumor suppressor gene regions (3p21-25, 11p15, 13q14, 17p11-13, and 18q21) was combined with immunohistochemical using Rb-PMG3-245 monoclonal antibody directed at the retinoblastoma (Rb) gene product. Tumor grade correlated with deletions of 3p (P = 0.004) and 17p (P = 0.063). Tumor stage correlated with deletions of 3p (P = 0.010), 17p (P = 0.015) and altered Rb expression (P = 0.054). Vascular invasion correlated only with deletions of 17p (P = 0.038). No marker correlated with positive lymph nodes. Our results suggest that altered Rb expression occurs in all grades and stages of bladder cancer but is more commonly associated with invasive tumors. Genetic alterations of 3p, 11p, 17p, and 18q are rare events in low grade, superficial tumors, whereas they are more common in high grade and invasive bladder cancer. The role of these genetic alterations in the prognosis of bladder cancer will require additional follow-up and further studies.
Author GALAN, T
REUTER, V. E
FAIR, W. R
PRESTI, J. C
CORDON-CARDO, C
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Issue 19
Keywords Chromosomal aberration
Histological grading
Human
Immunohistochemistry
Urinary system disease
Pathogenesis
Partial deletion
Abnormal E18 chromosome
Abnormal A3 chromosome
Malignant tumor
Suppressor gene
Pathology
Clinical stage
Restriction fragment length polymorphism
Urinary bladder
Abnormal chromosome
Abnormal C11 chromosome
Abnormal D13 chromosome
Abnormal E17 chromosome
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PublicationTitle Cancer research (Chicago, Ill.)
PublicationTitleAlternate Cancer Res
PublicationYear 1991
Publisher American Association for Cancer Research
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Snippet We attempted to define the role of tumor suppressor genes in the pathogenesis of human bladder cancer through a combined molecular genetic and...
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StartPage 5405
SubjectTerms Biological and medical sciences
Blotting, Southern
carcinoma
Chromosome Deletion
Chromosomes, Human, Pair 11
Chromosomes, Human, Pair 13
Chromosomes, Human, Pair 17
Chromosomes, Human, Pair 18
Chromosomes, Human, Pair 3
DNA - analysis
Genes, Tumor Suppressor
Humans
Immunohistochemistry
Medical sciences
Neoplasm Invasiveness
Neoplasm Staging
Nephrology. Urinary tract diseases
Polymorphism, Restriction Fragment Length
restriction fragment length polymorphism
retinoblastoma
Retinoblastoma Protein - biosynthesis
Tumors of the urinary system
urinary bladder
Urinary Bladder Neoplasms - genetics
Urinary Bladder Neoplasms - metabolism
Urinary tract. Prostate gland
Title Molecular genetic alterations in superficial and locally advanced human bladder cancer
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