Zebra chorionic gonadotropin: partial purification and characterization

• Published in: Biology of reproduction Vol. 44; no. 5; pp. 827 - 833
• Main Authors: MCFARLANE, J. R, CZEKALA, N. M, PAPKOFF, H
• Format: Journal Article
• Language: English
• Published: Madison, WI Society for the Study of Reproduction 01.05.1991
• Subjects: Analytical, structural and metabolic biochemistry; Animals; Biological and medical sciences; Biological Assay; chorionic gonadotropin; Chorionic Gonadotropin - blood; Chorionic Gonadotropin - isolation & purification; Chromatography, High Pressure Liquid; Female; Follicle Stimulating Hormone - blood; Fundamental and applied biological sciences. Psychology; Luteinizing Hormone - blood; Perissodactyla - blood; Pregnancy; Pregnancy, Animal - blood; Protein hormones. Growth factors. Cytokines; Proteins; Radioimmunoassay; Radioligand Assay; Species Specificity; Characterization; Vertebrata; Mammalia; Chorionic gonadotropin; Purification; Radioimmunoassay; HPLC chromatography; Artiodactyla; Glycoprotein hormone; Ungulata; Biological activity; Placental hormone
• DOI: 10.1095/biolreprod44.5.827
• ISSN: 0006-3363

Six samples of pregnant zebra (z) serum from the first and second trimesters of pregnancy were analyzed by RIA and shown to have chorionic gonadotropin levels comparable to that of the mare (0.9-5.3 micrograms/ml); first trimester levels in most cases were higher than second trimester levels. A pool of the sera (10 ml) was fractionated by methods previously employed for the purification of equine (e) and donkey (d) chorionic gonadotropin to achieve a concentration of the zebra chorionic gonadotropin (zCG). A yield of 1.0 mg of glycoprotein was obtained. HPLC analysis of the material indicated the content of zCG to be about 7%. Its molecular size as judged by Ve/Vo values is smaller than eCG, greater than ovine LH, and about the same as equine LH. The zCG was tested in RIAs for LH and eCG, radioreceptor assays (RRA) for LH and FSH, and the rat testis Leydig cell assay for LH. Comparisons were made with equine and donkey chorionic gonadotropin, and equine and zebra LH. The results, preliminary because the preparation is not of high purity, showed that zCG is bioactive as an LH; immunologically similar to eCG, eLH, dCG, and zLH; and competes in RRAs for LH but not FSH receptors. It differs, therefore, from eCG and eLH--which have high levels of intrinsic FSH activity, and is more like dCG, dLH, and zLH--all of which have minimal if any FSH activity.